Current Microbiology

, Volume 63, Issue 2, pp 213–217

Actimicrobium antarcticum gen. nov., sp. nov., of the Family Oxalobacteraceae, Isolated from Antarctic Coastal Seawater

  • Eun Hye Kim
  • Hyun-Jeong Jeong
  • Yoo Kyoung Lee
  • Eun Young Moon
  • Jang-Cheon Cho
  • Hong Kum Lee
  • Soon Gyu Hong
Article

Abstract

A Gram-negative, non-motile, catalase- and oxidase- positive, strictly aerobic, and short rod-shaped bacterium that was designated strain KOPRI 25157T was isolated from coastal seawater sample in Antarctica. The temperature and pH ranges for growth on R2A agar were 10–20°C, and 5.0–10.0, respectively. Phylogenetic analyses of the 16S rRNA gene sequence of strain KOPRI 25157T showed it to belong to the family Oxalobacteraceae of the class Betaproteobacteria, and it formed a distinct clade from other recognized members of the family. DNA G + C content was 65.9 mol%. Major ubiquinone was Q-8. Predominant cellular fatty acids were C16:1ω7c/15 iso 2OH (56.4%) and C16:1 (30.5%). Major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and unknown lipid. On the basis of these data, it is proposed that strain KOPRI 25157T is the representative of a novel genus, for which the name Actimicrobium gen. nov. is proposed in the family Oxalobacteraceae. The type strain for Actimicrobium antarcticum sp. nov. is KOPRI 25157T (=JCM 16673T=KCTC 23040T).

Supplementary material

284_2011_9962_MOESM1_ESM.pdf (20 kb)
Fig. S1 Two-dimensional thin-layer chromatogram of polar lipids of strain KOPRI 25157T. DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PS, phosphatidylserine; AL, unknown aminolipids; L, unknown polar lipid. (PDF 19 kb)

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Eun Hye Kim
    • 1
    • 2
  • Hyun-Jeong Jeong
    • 1
  • Yoo Kyoung Lee
    • 1
  • Eun Young Moon
    • 3
  • Jang-Cheon Cho
    • 2
  • Hong Kum Lee
    • 1
  • Soon Gyu Hong
    • 1
  1. 1.Division of Polar Life SciencesKorea Polar Research InstituteIncheonRepublic of Korea
  2. 2.Division of Biology and Ocean SciencesInha UniversityNam-gu, IncheonRepublic of Korea
  3. 3.Institute of MicrobiologySeoul National UniversitySeoulRepublic of Korea

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