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Current Microbiology

, Volume 63, Issue 2, pp 121–125 | Cite as

Identification of SACE_7040, a Member of TetR Family Related to the Morphological Differentiation of Saccharopolyspora erythraea

  • Shu Han
  • Ping Song
  • Ting Ren
  • Xunduan Huang
  • Cheng Cao
  • Buchang Zhang
Article

Abstract

SACE_7040 is presumed to be a member of the TetR family of transcriptional regulators in Saccharopolyspora erythraea, but its biological function is unknown. It was shown that the SACE_7040 gene knockout mutant formed aerial mycelium earlier than its original strain, and this phenotype could be restored by complementation of a single copy of SACE_7040 gene, demonstrating that SACE_7040 is an important regulator of the morphological differentiation of Sac. erythraea. When SACE_7040 gene was disrupted in the bldD mutant, we intriguingly found that the defect in aerial development exhibited by the bldD mutant could be overcome, suggesting a crosstalk between SACE_7040 and BldD in Sac. erythraea morphogenesis. These findings provide novel insights toward the Sac. erythraea developmental biology.

Keywords

Morphological Differentiation Aerial Mycelium Supplementary Material Figure Gene Deletion Mutant TetR Family 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We are grateful to Dr Yiguang Wang (Chinese Academy of Medical Sciences, Beijing, China) for providing Sac. erythraea A226, E.coli ET12567, pWHM3, and pSET152. We also thank Hang Wu (Shanghai Jiao Tong University, China) for helpful discussion. This study was supported by grants from National Natural Science Foundation of China (Grant Nos. 30870069) and National High-Tech Research and Development (863) Program (Grant Nos. 2007AA022204).

Supplementary material

284_2011_9943_MOESM1_ESM.doc (164 kb)
Supplementary material 1 (DOC 164 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.School of Life SciencesAnhui UniversityHefeiChina
  2. 2.Institute of BioengineeringAcademy of Military Medical SciencesBeijingChina

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