SACE_7040 is presumed to be a member of the TetR family of transcriptional regulators in Saccharopolyspora erythraea, but its biological function is unknown. It was shown that the SACE_7040 gene knockout mutant formed aerial mycelium earlier than its original strain, and this phenotype could be restored by complementation of a single copy of SACE_7040 gene, demonstrating that SACE_7040 is an important regulator of the morphological differentiation of Sac. erythraea. When SACE_7040 gene was disrupted in the bldD mutant, we intriguingly found that the defect in aerial development exhibited by the bldD mutant could be overcome, suggesting a crosstalk between SACE_7040 and BldD in Sac. erythraea morphogenesis. These findings provide novel insights toward the Sac. erythraea developmental biology.
Morphological Differentiation Aerial Mycelium Supplementary Material Figure Gene Deletion Mutant TetR Family
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We are grateful to Dr Yiguang Wang (Chinese Academy of Medical Sciences, Beijing, China) for providing Sac. erythraea A226, E.coli ET12567, pWHM3, and pSET152. We also thank Hang Wu (Shanghai Jiao Tong University, China) for helpful discussion. This study was supported by grants from National Natural Science Foundation of China (Grant Nos. 30870069) and National High-Tech Research and Development (863) Program (Grant Nos. 2007AA022204).
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