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Current Microbiology

, 63:16 | Cite as

Presence of qacEΔ1 Gene and Susceptibility to a Hospital Biocide in Clinical Isolates of Pseudomonas aeruginosa Resistant to Antibiotics

  • Célia RomãoEmail author
  • Catia Aparecida Miranda
  • Jaqueline Silva
  • Maysa Mandetta Clementino
  • Ivano de Filippis
  • Marise Asensi
Article

Abstract

Biocides play an important role in healthcare-associated infection control by either minimizing or preventing microorganism dissemination. This study evaluated the susceptibility of Pseudomonas aeruginosa clinical isolates to a quaternary ammonium (QAC) disinfectant and antibiotics, and verified the presence of qacEΔ1, a determinant of resistance to QAC. The disinfectant test was the Association of Official Analytical Chemists Use-Dilution Test, and polymerase chain reaction was used to examine for qacEΔ1. The qacEΔ1 gene was detected in 48% of the isolates. Eighty-eight percent of the multiresistant isolates carried qacEΔ1 gene, while 35% of the non-multiresistant isolates was positive to this gene, and multiresistance well correlated with its presence. Among isolates tested for the disinfectant, 46% showed a reduced susceptibility to the disinfectant. qacEΔ1 gene was present in 70% of the susceptible isolates to the biocide, whereas 90% of the less susceptible strains harbored this gene. Reduced susceptibility to the disinfectant was independent of presence of qacEΔ1 suggesting that it does not play an important role in biocide resistance in P. aeruginosa. As far as we know, it is the first report confirming this fact and testing with disinfectant at its in-use concentration. The evidence of less susceptible strains than the reference bacterium used in disinfectant testing, and the high percentage of qacEΔ1 gene detected are of special concern and suggests continued investigation in laboratory and in situ, not only in healthcare settings, but also in all areas of biocide usage, including different micro-organisms and biocides.

Keywords

Imipenem Biocide Cefepime Aztreonam Susceptible Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Altschult SF, Maddelen TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402CrossRefGoogle Scholar
  2. 2.
    Beloian A (1990) Disinfectants. In: Association of official analytical chemists. Official methods of analysis, cap. 6, 15 edn. Arlington, WashingtonGoogle Scholar
  3. 3.
    Cezário RC, De Morais LD, Ferreira JC, Costa Pinto RM, Darin ALC, Gontijo-Filho PP (2009) Nosocomial outbreak by imipenem-resitant metallo-ß-lactamase producing Pseudomonas aeruginosa in an adult intensive care unit in a Brazilian teaching hospital. Enferm Infec Microbiol Clin 27:269–274CrossRefGoogle Scholar
  4. 4.
    Chuanchuen R, Khemtong S, Padungtod P (2007) Ocurrence of qacE/qacEΔ1 genes and their correlation with class1 integrons in Salmonella enteric isolates from poultry and swine. South Asian J Trop Med Public Health 38:855–862Google Scholar
  5. 5.
    Clinical and Laboratory Standards Institute (2008) Performance standards for antimicrobial susceptibility tests; eighteenth informational supplement. CLSI document M100–S18. Clinical and Laboratory Standards Institute, Wayne, PAGoogle Scholar
  6. 6.
    Gaze WH, Abdouslam N, Hawkey PM, Wellington EMH (2005) Incidence of class 1 integrons in a quaternary ammonium compound-polluted environment. Antimicrob Agents Chemother 49:1802–1807PubMedCrossRefGoogle Scholar
  7. 7.
    Hegstad K, Langsrud S, Lunestad BT, Scheie AA, Sunde M, Yazdankhah SP (2010) Does the wide use of quaternary ammonium compounds enhance the selection and spread of antimicrobial resistance and thus threaten our health? Microb Drug Resist 16:91–104PubMedCrossRefGoogle Scholar
  8. 8.
    Herruzo-Cabrera R, Vizcaino-Alcaide MJ, Fernández-Aceñero MJ (2004) The influence of laboratory adaptation on test strains, such as Pseudomonas aeruginosa, in the evaluation of the antimicrobial efficacy of ortho-phthalaldehyde. J Hosp Infect 57:217–222PubMedCrossRefGoogle Scholar
  9. 9.
    Kazama H, Hamashima H, Sasatsu M, Arai T (1998) Distribution of the antiseptic-resistance genes qacE and qacEΔ1 in Gram-negative bacteria. FEMS Microbiol Lett 59:173–178Google Scholar
  10. 10.
    Kohlenberg A, Weitzel-Kage D, van der Linden P, Sohr D, Vögeler S, Kola A, Halle E, Rüden H, Weist K (2010) Outbreak of carbapenem-resistant Pseudomonas aeruginosa infection in a surgical intensive care unit. J Hosp Infect 74:350–357PubMedCrossRefGoogle Scholar
  11. 11.
    Kücken D, Heinz-Hubert F, Kaukfers P-M (2000) Association of qacE and qacEΔ1 with multiple resistance to antibiotics and antiseptics in clinical isolates of Gram-negative bacteria. FEMS Microbiol Lett 183:95–98PubMedCrossRefGoogle Scholar
  12. 12.
    Langsrud S, Sidhu MS, Heir E, Holck A (2003) Bacterial disinfectant resistance—a challenge for the food industry. Inter Bio Biodegr 51:283–290CrossRefGoogle Scholar
  13. 13.
    Maillard J-Y (2007) Bacterial resistance to biocides in the healthcare environment: should it be of genuine concern? J Hosp Infect 65(S2):60–72PubMedCrossRefGoogle Scholar
  14. 14.
    McDonnell G, Russell AD (1999) Antiseptics and disinfectants: activity, action and resistance. Clin Microbiol Rev 12:47–179Google Scholar
  15. 15.
    Paulsen IT, Littlejohn TG, Rådström P, Sundström L, Sköld O, Swedberg G, Skurray LA (1993) The 3′ conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants. Antimicrob Ag Chemother 37:761–768Google Scholar
  16. 16.
    Paulsen IT, Brown MH, Skurray RA (1996) Proton-dependent multidrug efflux systems. Microbiol Rev 60:575–608PubMedGoogle Scholar
  17. 17.
    Poole K (2002) Mechanism of bacterial biocide and antibiotic resistance. J Appl Microbiol Symp 92(Suppl):55S–64SCrossRefGoogle Scholar
  18. 18.
    Romão CMCPA, Faria YN, Pereira LR, Asensi MD (2005) Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing. Mem Inst Oswaldo Cruz 100:541–548PubMedCrossRefGoogle Scholar
  19. 19.
    Russell AD (1999) Bacterial resistance to disinfectants: present knowledge and future problems. J Hosp Infect 43(Suppl.):S57–S68PubMedCrossRefGoogle Scholar
  20. 20.
    Russell AD (2001) Mechanisms of bacterial insusceptibility to biocides. AJIC 29:259–261Google Scholar
  21. 21.
    Russell AD (2004) Bacterial adaptation and resistance to antiseptics, disinfectant and preservatives is not a new phenomen. J Hosp Infect 57:97–104PubMedCrossRefGoogle Scholar
  22. 22.
    Rutala WA, Weber DJ (2008) Healthcare infection control practices advisory committee, guideline for disinfection and sterilization in healthcare facilities. [http://www.cdc.gov/HAI/prevent/prevent_pubs.html], CDC, USA
  23. 23.
    Silva Filho LVF, Levi JE, Bento CNO, Rodrigues JC, Ramos SRTS (2001) Molecular epidemiology of Pseudomonas aeruginosa infections in a cystic fibrosis outpatient clinic. J Med Microbiol 50:261–267PubMedGoogle Scholar
  24. 24.
    Strateva T, Yordanov D (2009) Pseudomonas aeruginosa—a phenomenon of bacterial resistance. J Med Microb 58:1133–1148CrossRefGoogle Scholar
  25. 25.
    Tomasino S (2007) Disinfectants. In: Association of Official Analytical Chemists. Official Methods of Analysis, cap. 6, 18 edn. Rev 2. Gaithersburg, MarylandGoogle Scholar
  26. 26.
    Wang C, Zhan Q, Mi Z, Huang Z, Chen G (2008) Distribution of the antiseptic-resistance gene qacEΔ1 in 283 clinical isolates of Gram-negative bacteria in China. J Hosp Infec 69:394–396CrossRefGoogle Scholar
  27. 27.
    Weber DJ, Rutala WA (2006) Use of germicides in the home and the healthcare setting: is there a relationship between germicide use and antibiotic resistance? Infec Control Hosp Epidemiol 27:1107–1119CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Célia Romão
    • 1
    Email author
  • Catia Aparecida Miranda
    • 1
  • Jaqueline Silva
    • 1
  • Maysa Mandetta Clementino
    • 1
  • Ivano de Filippis
    • 1
  • Marise Asensi
    • 2
  1. 1.Department of MicrobiologyINCQS-Fundação Oswaldo CruzRio de JaneiroBrazil
  2. 2.IOC-Fundação Oswaldo CruzRio de JaneiroBrazil

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