Bactofection with Toll-Like Receptor 4 in a Murine Model of Urinary Tract Infection
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The role of innate immunity in the prevention of urinary tract infection is well-documented. Toll-like receptor 4 (TLR4) is a major determinant of innate immune response. In an animal model of urinary tract infection, bactofection-mediated gene transfer of TLR4 was tested in a preventive approach. Bactofection with TLR4 reduced the colonization with uropathogenic Escherichia coli by 91% in the kidney and by 41% in the bladder. Reduced colonization was associated with lower oxidative stress and expression of monocyte chemoattractant protein-1 and myeloperoxidase in the kidney. Bactofection with TLR4 was successful in the prevention of ascending pyelonephritis. Further studies should focus on long-term effects, the dose response and the potential therapeutic use in models of chronic urinary tract infection.
KeywordsUrinary Tract Infection TLR4 Expression Advance Oxidation Protein Product Gene Therapy Approach Transurethral Catheterization
This study was funded by the Slovak Research and Development Agency (SRDA)—grant APVV-0117-07. The authors are grateful to Prof. Ivan Pecháň (1932–2010) for great help in establishing the analysis of oxidative stress markers.
Conflict of interest
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