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Current Microbiology

, Volume 62, Issue 6, pp 1739–1742 | Cite as

Bactofection with Toll-Like Receptor 4 in a Murine Model of Urinary Tract Infection

  • Ľubomíra Tóthová
  • Július Hodosy
  • Natália Kamodyová
  • Pavol Janega
  • Lívia Slobodníková
  • Adriana Liptáková
  • Peter Boor
  • Peter CelecEmail author
Article

Abstract

The role of innate immunity in the prevention of urinary tract infection is well-documented. Toll-like receptor 4 (TLR4) is a major determinant of innate immune response. In an animal model of urinary tract infection, bactofection-mediated gene transfer of TLR4 was tested in a preventive approach. Bactofection with TLR4 reduced the colonization with uropathogenic Escherichia coli by 91% in the kidney and by 41% in the bladder. Reduced colonization was associated with lower oxidative stress and expression of monocyte chemoattractant protein-1 and myeloperoxidase in the kidney. Bactofection with TLR4 was successful in the prevention of ascending pyelonephritis. Further studies should focus on long-term effects, the dose response and the potential therapeutic use in models of chronic urinary tract infection.

Keywords

Urinary Tract Infection TLR4 Expression Advance Oxidation Protein Product Gene Therapy Approach Transurethral Catheterization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was funded by the Slovak Research and Development Agency (SRDA)—grant APVV-0117-07. The authors are grateful to Prof. Ivan Pecháň (1932–2010) for great help in establishing the analysis of oxidative stress markers.

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ľubomíra Tóthová
    • 1
    • 2
  • Július Hodosy
    • 1
    • 3
  • Natália Kamodyová
    • 1
  • Pavol Janega
    • 4
    • 5
  • Lívia Slobodníková
    • 6
  • Adriana Liptáková
    • 7
  • Peter Boor
    • 8
    • 9
  • Peter Celec
    • 1
    • 2
    • 10
    Email author
  1. 1.Institute of Molecular BiomedicineComenius UniversityBratislavaSlovakia
  2. 2.Department of Molecular BiologyComenius UniversityBratislavaSlovakia
  3. 3.Institute of PhysiologyComenius UniversityBratislavaSlovakia
  4. 4.Institute of PathologyComenius UniversityBratislavaSlovakia
  5. 5.Institute of Normal and Pathological PhysiologySlovak Academy of ScienceBratislavaSlovakia
  6. 6.Institute of MicrobiologyComenius UniversityBratislavaSlovakia
  7. 7.Institute of ImmunologyComenius UniversityBratislavaSlovakia
  8. 8.Department of Nephrology and Institute of PathologyRWTH University of AachenAachenGermany
  9. 9.Institute of Molecular Biomedicine, Faculty of MedicineComenius UniversityBratislavaSlovakia
  10. 10.Institute of PathophysiologyComenius UniversityBratislavaSlovakia

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