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Current Microbiology

, Volume 62, Issue 6, pp 1732–1738 | Cite as

Chitinolyticbacter meiyuanensis SYBC-H1T, Gen. Nov., sp. Nov., a Chitin-Degrading Bacterium Isolated From Soil

  • Zhikui Hao
  • Yujie Cai
  • Xiangru LiaoEmail author
  • Xiaohui Liang
  • Jiayang Liu
  • Zhiyou Fang
  • Mingming Hu
  • Dabing Zhang
Article

Abstract

A novel aerobic mesophilic bacterial strain SYBC-H1T capable of degrading chitin was isolated and classified in this study. The strain exhibited strong chitinolytic activity and was a Gram-negative, curved, rod-shaped, and motile bacterium. Growth of this strain was observed between 10 and 41°C and between pH 3.5 and 9.5. The DNA G + C content of strain SYBC-H1T was 53.25 mol%. The cellular fatty acids (>5%) were 12:0 iso 3-OH (5.87%), 16:0 (28.16%), and 18:1ω7c (20.48%). Phylogenetic analysis based on 16S rRNA gene sequence similarity revealed that strain SYBC-H1T belonged to the family Neisseriaceae, and was distantly related (95.0% similarity) to the genus Chitiniphilus. Its phenotype was unique and genetic and phylogenetic analysis experiments suggested that strain SYBC-H1T represented the type strain (CGMCC 3438T, ATCC BAA-2140T) of a novel genus, for which the name Chitinolyticbacter meiyuanensis SYBC-H1T gen. nov., sp. nov. was proposed. The highest enzymatic activity of chitinase (9.6 U/ml) was obtained at 72 h in 250 ml shake flasks. The 16S rRNA gene sequence of SYBC-H1T has been deposited in GenBank under the accession number GQ981314.

Keywords

Chitin Chitinase GlcNAc Itaconic Acid Chitinase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was financially supported by the National High Technology and Development Program of China (863 Program, Grant no. 2010AA101501) and National Institutes of Health grant GM-33048.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Zhikui Hao
    • 1
    • 2
  • Yujie Cai
    • 1
  • Xiangru Liao
    • 1
    Email author
  • Xiaohui Liang
    • 3
  • Jiayang Liu
    • 1
  • Zhiyou Fang
    • 4
  • Mingming Hu
    • 1
  • Dabing Zhang
    • 5
  1. 1.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuxiPeople’s Republic of China
  2. 2.Fishery Extension Station of Yingzhou DistrictFuyangPeople’s Republic of China
  3. 3.Energy Research Institute of Shandong Academy of SciencesJinanPeople’s Republic of China
  4. 4.Department of Cell Biology and Cell Dynamics ProgramUniversity of Massachusetts Medical SchoolWorcesterUSA
  5. 5.Jiangsu Hanbon Science & Technology Co. LtdHuai’anPeople’s Republic of China

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