The Intestinal Bacterial Community in the Food Waste-Reducing Larvae of Hermetia illucens
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As it is known that food waste can be reduced by the larvae of Hermetia illucens (Black soldier fly, BSF), the scientific and commercial value of BSF larvae has increased recently. We hypothesised that the ability of catabolic degradation by BSF larvae might be due to intestinal microorganisms. Herein, we analysed the bacterial communities in the gut of BSF larvae by pyrosequencing of extracting intestinal metagenomic DNA from larvae that had been fed three different diets. The 16S rRNA sequencing results produced 9737, 9723 and 5985 PCR products from larval samples fed food waste, cooked rice and calf forage, respectively. A BLAST search using the EzTaxon program showed that the bacterial community in the gut of larvae fed three different diets was mainly composed of the four phyla with dissimilar proportions. Although the composition of the bacterial communities depended on the different nutrient sources, the identified bacterial strains in the gut of BSF larvae represented unique bacterial species that were unlike the intestinal microflora of other insects. Thus, our study analysed the structure of the bacterial communities in the gut of BSF larvae after three different feedings and assessed the application of particular bacteria for the efficient degradation of organic compounds.
KeywordsBacterial community Pyrosequencing Hermetia illucens Phylogenetic analysis Black soldier fly (BSF)
This study was supported by the Rural Development Administration, Korea (PJ006893). H. Jeon was supported by a graduate school scholarship from Kyung Hee University. Pyrosequencing data were analysed by the Chun Laboratory at Seoul National University.
- 14.Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- 18.Hongoh Y, Deevong P, Inoue T, Moriya S, Trakulnaleamsai S, Ohkuma M, Vongkaluang C, Noparatnaraporn N, Kudo T (2005) Intra- and interspecific comparisons of bacterial diversity and community structure support coevolution of gut microbiota and termite host. Appl Environ Microbiol 71:6590–6599PubMedCrossRefGoogle Scholar
- 21.Jung YJ, Jung D, Kim JY, Zo YG, Yim JH, Lee H, Ahn TS (2009) Distribution of bacterial decomposers in lake Khuvsgul, Mongolia. Korean J Microbiol 45:119–125 (Korean)Google Scholar
- 22.Kim JG, Choi YC, Choi JY, Kim WT, Jeong GS, Park KH, Hwang SJ (2008) Ecology of the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae) in Korea. Korean J Appl Entomol 47:337–343 (Korean)Google Scholar
- 27.Lee HK, Ahn MJ, Kwak SH, Song WH, Jeong BC (2003) Purification and characterization of cold active lipase from psychotropic Aeromonas sp. LPB 4. J Microbiol 41:22–27Google Scholar
- 28.Liu Q, Tomberlin JK, Brady JA, Sanford MR, Yu Z (2008) Black soldier fly (Diptera: Stratiomyidae) larvae reduce Escherichia coli in dairy manure. Environment 37:1525–1530Google Scholar
- 43.Teather RM, Wood PJ (1982) Use of Congo red polysaccharide interactions complex formation between Congo red and polysaccharide in detection and assay of polysaccharide hydrolases. Methods Enzymol 160:59–74Google Scholar