Current Microbiology

, Volume 64, Issue 1, pp 43–49 | Cite as

Prevalence and Isoforms of the Pathogenicity Island ETT2 Among Escherichia coli Isolates from Colibacillosis in Pigs and Mastitis in Cows

  • DaRong ChengEmail author
  • ShanYuan Zhu
  • ZhiRui Su
  • WeiYong Zuo
  • Hui Lu


To study the prevalence and isoforms of the pathogenicity island ETT2 among pathogenic Escherichia coli, as well as to determine the relationship between the ETT2 locus and other virulence factors, PCR amplifications target to the 35 ETT2-associated genes were established and used to investigate the presence of the ETT2 locus in 168 E. coli isolates from weaned piglets with edema and/or diarrhea or dairy cows with mastitis. The results showed that the ETT2 locus could be identified in the pathogenic E. coli isolates from colibacillosis in pigs and in the ones from mastitis in cows, but the presence of ETT2 among the isolates of porcine origin were significantly higher (85.87%) than that (47.37%) of bovine origin. Furthermore, 11 ETT2 isoforms were found in this research, including an intact form and 10 deletion types. The intact ETT2 was the prevalent form among the pathogenic E. coli isolates of porcine origin, and highly associated with the presence of shigatoxin type 2e (Stx2e), while the great majority isolates of bovine origin just carried various deletion types, and no distinct association with other virulence factors, e.g., the presence/absence of LT1, ST2, Cnf2, Tra, HPI, Hly, and F17a fimbriae.


Escherichia coli ETT2 Prevalence Type Piglet Cow 



This study was supported by Jiangsu Province Science and Technology Support Program (Agriculture) (Grant No. BE2010381), Natural Science Foundation of Jiangsu Province (Grant No. BK2009738), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions. We would like to give our thanks to all the staff of the veterinary microbiology laboratory of Yangzhou University for their help with some experiments.


  1. 1.
    Blanc-Potard AB, Solomon F, Kayser J, Groisman EA (1999) The SPI-3 pathogenicity island of Salmonella enterica. J Bacteriol 1999(181):998–1004Google Scholar
  2. 2.
    Cheng D, Sun H, Xu J, Gao S (2005) Prevalence of fimbial colonization factors F18ab and F18ac in Escherichia coli isolates from weaned piglets with edema and/or diarrhea in China. Vet Microbiol 110:35–39PubMedCrossRefGoogle Scholar
  3. 3.
    Cheng D, Sun H, Xu J, Gao S (2006) PCR detection of virulence factor genes in Escherichia coli isolates from weaned piglets with edema disease and/or diarrhea in China. Vet Microbiol 115:320–328PubMedCrossRefGoogle Scholar
  4. 4.
    Cheng DR, Zhu SY, Ding WW, Chen XL, Gao XP, Sun HC (2010) Evaluation of the efficacy of maternal vaccination by using dominant pathogenic Escherichia coli isolates to control neonatal diarrhea in individual swine farm. Pure Appl Microbiol 4:487–495Google Scholar
  5. 5.
    Cheng DR, Zhu SY, Yin ZH, Ding WW, Mu ZX, Su ZR, Sun HC (2010) Prevalence of bacterial infection responsible for bovine mastitis. Afr J Microbiol Res 4:1110–1116Google Scholar
  6. 6.
    Fekete PZ, Gerardin J, Jacquemin E, Mainil JG, Nagy B (2002) Replicon typing of F18 fimbriae encoding plasmids of enterotoxigenic and verotoxigenic Escherichia coli strains from porcine postweaning diarrhoea and oedema disease. Vet Microbiol 85:275–284PubMedCrossRefGoogle Scholar
  7. 7.
    Galán JE (1996) Molecular genetic bases of Salmonella entry into host cells. Mol Microbiol 20:263–271PubMedCrossRefGoogle Scholar
  8. 8.
    Hacker J, Kaper JB (2000) Pathogenicity islands and the evolution of microbes. Annu Rev Microbiol 54:641–679PubMedCrossRefGoogle Scholar
  9. 9.
    Hansen-Wester I, Hensel M (2001) Salmonella pathogenicity islands encoding type III secretion systems. Microbes Infect 3:549–559PubMedCrossRefGoogle Scholar
  10. 10.
    Hayashi T, Makino K, Ohnishi M, Kurokawa K, Ishii K, Yokoyama K, Han CG, Ohtsubo E, Nakayama K, Murata T, Tanaka M, Tobe T, Iida T, Takami H, Honda T, Sasakawa C, Ogasawara N, Yasunaga T, Kuhara S, Shiba T, Hattori M, Shinagawa H (2001) Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12. DNA Res 8:11–22PubMedCrossRefGoogle Scholar
  11. 11.
    Nagy B, Fekete PZ (1999) Enterotoxigenic Escherichia coli (ETEC) in farm animals. Vet Res 30:259–284PubMedGoogle Scholar
  12. 12.
    Osawa K, Shibata M, Nishiyama Y, Kurokawa M, Yamamoto G, Kinoshita S, Kataoka N (2006) Identification of the ETT2 locus in human diarrheagenic Escherichia coli by multiplex PCR. J Infect Chemother 12:157–159PubMedCrossRefGoogle Scholar
  13. 13.
    Perna NT, Plunkett G III, Burland V, Mau B, Glasner JD, Rose DJ, Mayhew GF, Evans PS, Gregor J, Kirkpatrick HA, Pósfai G, Hackett J, Klink S, Boutin A, Shao Y, Miller L, Grotbeck EJ, Davis NW, Lim A, Dimalanta ET, Potamousis KD, Apodaca J, Anantharaman TS, Lin J, Yen G, Schwartz DC, Welch RA, Blattner FR (2001) Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409:529–533PubMedCrossRefGoogle Scholar
  14. 14.
    Prager R, Bauerfeind R, Tietze E, Behrend J, Fruth A, Tschäpe H (2004) Prevalence and deletion types of the pathogenicity island ETT2 among Escherichia coli strains from oedema disease and colibacillosis in pigs. Vet Microbiol 99:287–294PubMedCrossRefGoogle Scholar
  15. 15.
    Ren CP, Chaudhuri RR, Fivian A, Bailey CM, Antonio M, Barnes WM, Pallen MJ (2004) The ETT2 gene cluster, encoding a second type III secretion system from Escherichia coli, is present in the majority of strains but has undergone widespread mutational attrition. J Bacteriol 186:3547–3560PubMedCrossRefGoogle Scholar
  16. 16.
    Rippinger P, Bertschinger HU, Imberechts H, Nagy B, Sorg I, Stamm M, Wild P, Wittig W (1995) Designations F18ab and F18ac for the related fimbrial types F107, 2134P and 8813 of Escherichia coli isolated from porcine postweaning diarrhoea and from oedema disease. Vet Microbiol 45:281–295PubMedCrossRefGoogle Scholar
  17. 17.
    Salajka E, Salajkova Z, Alexa P, Hornich M (1992) Colonization factor different from K88, K99, F41 and 987P in enterotoxigenic Escherichia coli strains isolated from postweaning diarrhoea in pigs. Vet Microbiol 32:163–175PubMedCrossRefGoogle Scholar
  18. 18.
    Yao Y, Xie Y, Perace D, Zhong Y, Lu J, Tao J, Guo X, Kim KS (2009) The type III secretion system is involved in the invasion and intracellular survival of Escherichia coli K1 in human brain microvascular endothelial cells. FEMS Microbiol Lett 300:18–24PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • DaRong Cheng
    • 1
    Email author
  • ShanYuan Zhu
    • 2
  • ZhiRui Su
    • 1
  • WeiYong Zuo
    • 2
  • Hui Lu
    • 2
  1. 1.College of Veterinary MedicineYangzhou UniversityYangzhouChina
  2. 2.Jiangsu Animal Husbandry and Veterinary CollegeTaizhouChina

Personalised recommendations