Current Microbiology

, 63:517 | Cite as

Changing Patterns of Rotavirus Genotypes in Turkey

  • Anil Tapisiz
  • Zeynep Ceren Karahan
  • Ergin Çiftçi
  • Erdal İnce
  • Ülker Doğru
Article

Abstract

To describe the circulation dynamics of human rotavirus genotypes and to understand the epidemiological changes of rotavirus infection in Turkey, one of the countries where the highest mortality rates are seen due to rotavirus in Europe. Stool samples of children under 5 years of age which gave positive results for rotavirus antigen were stored at −20°C and then genotyped using multiplex reverse transcription polymerase-chain reaction. Of the 494 stool samples, 137 (28.1%) were positive for rotavirus antigen and 100 (73%) samples which could be genotyped successfully were included in the study. 42 (42%) samples were from inpatients, and 58 (58%) were from outpatients. The median age of the children was 16 months (5 days–59 months). G9 and P[8] were the most frequent G and P genotypes, and were detected in 30 (30%) and 55 patients (55%), respectively. In 90 samples for which both G and P genotypes could be determined, 34 different combinations were found. G9P[8] was the most frequent genotype detected in 19 patients (19%), followed by G1P[8] and G4P[6] each in 7 (7%) patients. The incidence of mixed infection was found to be 26%. Novel strains like P2A[6] and P[5] and unusual reassortant strains were detected. Distribution of rotavirus genotypes exhibited distinctive changes in this study. When the everchanging epidemiology of rotaviruses is taken into account, ongoing surveillance studies are important before the inclusion of rotavirus vaccines in national immunization program of Turkey.

Notes

Acknowledgments

This study was supported by a fund obtained from the Ankara University Scientific Investigation projects office (Project No: 20080809264).

Conflict of interest

There is no conflict of interest to be declared among the authors.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Anil Tapisiz
    • 1
  • Zeynep Ceren Karahan
    • 2
  • Ergin Çiftçi
    • 1
  • Erdal İnce
    • 1
  • Ülker Doğru
    • 1
  1. 1.Department of Pediatric Infectious DiseaseAnkara University Medical SchoolDikimevi, AnkaraTurkey
  2. 2.Department of Microbiology and Clinical MicrobiologyAnkara University Medical SchoolAnkaraTurkey

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