Triazophos is one of the most widely used organophosphorus insecticides usually detectable in the environment. A bacterial strain, Diaphorobacter sp. TPD-1, capable of using triazophos and its intermediate, 1-phenyl-3-hydroxy-1,2,4-triazole (PHT), as its sole carbon sources for growth was isolated from a triazophos-contaminated soil in China. This strain could completely degrade 50 mg l−1 triazophos and PHT to non-detectable level in 24 and 56 h, respectively. During PHT degradation, three metabolites were detected and identified based on tandem mass spectrometry (MS/MS) analysis. Using this information, a biochemical degradation pathway of triazophos by Diaphorobacter sp. TPD-1 was proposed. The first step involved in the degradation of triazophos is the hydrolysis of the P–O ester bond of triazophos to form PHT and o,o-diethyl phosphorothioic acid, then the triazol ring of PHT is subsequently cleaved to form (E)-1-formyl-2-phenyldiazene. Subsequently, (E)-1-formyl-2-phenyldiazene is transformed to 2-phenylhydrazinecarboxylic acid by adding one molecular of H2O. Finally, the carboxyl group of 2-phenylhydrazinecarboxylic acid is decarboxylated to form phenylhydrazine.
Sole Carbon Source Phenylhydrazine Organophosphorus Insecticide Recording Spectrophotometer Triazophos
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This work was supported by grants from the Major Projects on Control and Rectification of Water Body Pollution (2009ZX07103-002), the Chinese National Natural Science Foundation (31070100), and the Key Technology R&D Program of Jiangsu Province (BE2008669).
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1.Department of Microbiology, Key Laboratory for Microbiological Engineering of Agricultural Environment of Ministry of Agriculture, College of Life SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China
2.Department of Bioengineering, School of Chemical EngineeringNanjing University of Science and TechnologyNanjingPeople’s Republic of China