Current Microbiology

, Volume 62, Issue 4, pp 1097–1103 | Cite as

In Vitro and In Vivo Antioxidant Activity of Bifidobacterium animalis 01 Isolated from Centenarians

  • Qian Shen
  • Nan Shang
  • Pinglan Li


Several studies reported the antioxidant activity of bifidobacteria using assays in vitro. In present study, the in vitro and in vivo antioxidant activity of Bifidobacterium animalis 01 was investigated. Culture supernatant, intact cells, and intracellular cell-free extracts of B. animalis 01 were involved in this study. The antioxidant assays in vitro included lipid peroxidation assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydroxyl radical ( OH) assay and superoxide anion (\( {\text{O}}_{2}^{ - } \)) assay. The antioxidant assays in vivo were conducted using mice model. Activities of antioxidative enzymes, malondialdehyde (MDA) content in serums and livers of aging mice were evaluated. Monoamine oxidase (MAO) activity and lipofuscin level in brains of aging mice were also characterized. Results showed that culture supernatant, intact cells and intracellular cell-free extracts of B. animalis 01 could effectively scavenge free radicals, significantly enhance mice’s activities of antioxidative enzymes and reduce mice’s MDA content, lipofuscin level and MAO activity. Our results indicated that B. animalis 01 has the potential to be developed into a dietary antioxidant supplements.


Antioxidant Activity Intact Cell Bifidobacterium Longum Model Control Group Gastric Gavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was funded by China National 863 Program (2008AA10Z324) and Beijing Natural Science Foundation (5072025).


  1. 1.
    Alper G, Girgin F, Ozgonol M, Mentes G, Ersoz B (1999) MAO inhibitors and oxidant stress in aging brain tissue. Eur Neuropsychopharmacol 9:247–252PubMedCrossRefGoogle Scholar
  2. 2.
    Andaloussi SA, Talbaoui H, Marczak R, Bonaly R (1995) Isolation and characterization of exocellular polysaccharides produced by Bifidobacterium longum. Appl Microbiol Biotechnol 43:995–1000CrossRefGoogle Scholar
  3. 3.
    Blois MS (2002) Antioxidant determinations by the use of a stable free radical. Nature 26:1199–1200Google Scholar
  4. 4.
    Bouhnik Y, Pochart P, Marteau P, Arlet G, Goderel I, Rambaud JC (1992) Fecal recovery in humans of viable Bifidobacterium sp. ingested in fermented milk. Gastroenterology 102:875–878PubMedGoogle Scholar
  5. 5.
    Buettner GR, Mason RP (1990) Spin-trapping methods for detecting superoxide and hydroxyl free radicals in vivo and in vitro. Methods Enzymol 186:127–133PubMedCrossRefGoogle Scholar
  6. 6.
    Grossman S, Zakut R (1979) Determination of the activity of lipoxygenase (lipoxidase). Methods Biochem Anal 25:303–329PubMedCrossRefGoogle Scholar
  7. 7.
    Jack RW, Tagg JR, Ray B (1995) Bacteriocins of Gram-positive bacteria. Microbiol Rev 59:171–200PubMedGoogle Scholar
  8. 8.
    Kim JY, Choi SI, Heo TR (2003) Screening of antioxidative activity of Bifidobacterium species isolated from Korean infants feces and their identification. Biotechnol Bioprocess Eng 8:199–204CrossRefGoogle Scholar
  9. 9.
    Kohno M, Suzuki S, Kanaya T, Yoshino T, Matsuura Y, Asada M, Kitamura S (2009) Structural characterization of the extracellular polysaccharide produced by Bifidobacterium longum JBL05. Carbohydr Polym 77:351–357CrossRefGoogle Scholar
  10. 10.
    Lin MY, Chang FJ (2000) Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356. Dig Dis Sci 45:1617–1622PubMedCrossRefGoogle Scholar
  11. 11.
    Lin MY, Yen CL (1999) Inhibition of lipid peroxidation by Lactobacillus acidophilus and Bifidobacterium longum. J Agric Food Chem 47:3661–3664PubMedCrossRefGoogle Scholar
  12. 12.
    Melov S, Ravenscroft J, Malik S, Gill MS, Walker DW, Clayton PE, Wallace DC, Malfroy B, Doctrow SR, Lithgow GJ (2000) Extension of life-span with superoxide dismutase/catalase mimetics. Science 289:1567–1569PubMedCrossRefGoogle Scholar
  13. 13.
    Meghrous J, Euloge P, Junelles AM, Ballongue J, Petitdemange H (1990) Screening of Bifidobacterium strains for bacteriocin production. Biotechnol Lett 12:575–580CrossRefGoogle Scholar
  14. 14.
    Mitsuoka T (1990) Bifidobacteria and their role in human health. J Ind Microbiol 6:263–268CrossRefGoogle Scholar
  15. 15.
    Nicol S (1987) Some limitations on the use of the lipofuscin ageing techniques. Mar Biol 93:609–614CrossRefGoogle Scholar
  16. 16.
    Saikali J, Picard C, Freitas M, Holt P (2004) Fermented milks, probiotic cultures, and colon cancer. Nutr Cancer 49:14–24PubMedCrossRefGoogle Scholar
  17. 17.
    Sartor RB (2004) Therapeutic manipulation of the enteric microflora in inflammatory bowel diseases: antibiotics, probiotics, and prebiotics. Gastroenterology 126:1620–1633PubMedCrossRefGoogle Scholar
  18. 18.
    Seifried HE, Anderson DE, Fisher EI, Milner JA (2007) A review of the interaction among dietary antioxidants and reactive oxygen species. J Nutr Biochem 18:567–579PubMedCrossRefGoogle Scholar
  19. 19.
    Sharma D, Maurya AK, Singh R (1993) Age-related decline in multiple unit action potentials of CA3 region of rat hippocampus: correlation with lipid peroxidation and lipofuscin concentration and the effect of centrophenoxine. Neurobiol Aging 14:319–330PubMedCrossRefGoogle Scholar
  20. 20.
    Shen Q, Zhang B, Xu R, Wang Y, Ding X, Li P (2010) Antioxidant activity in vitro of the selenium-contained protein from the Se-enriched Bifidobacterium animalis 01. Anaerobe 16:380–386PubMedCrossRefGoogle Scholar
  21. 21.
    Tanas S, Odabasoglu F, Halici Z, Cakir A, Aygun H, Ali A, Suleyman H (2010) Evaluation of anti-inflammatory and antioxidant activities of Peltigera rufescens lichen species in acute and chronic inflammation models. J Nat Med 64:42–49PubMedCrossRefGoogle Scholar
  22. 22.
    Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell B 39:44–84CrossRefGoogle Scholar
  23. 23.
    Vernet M, Hunter JR, Vetter RD (1988) Accumulation of age-pigments 522 (lipofuscin) in two cold-water fishes. Fish Bull 86:401–407Google Scholar
  24. 24.
    Virtanen T, Pihlanto A, Akkanen S, Korhonen H (2007) Development of antioxidant activity in milk whey during fermentation with lactic acid bacteria. J Appl Microbiol 102:106–115PubMedCrossRefGoogle Scholar
  25. 25.
    Wang YC, Yu RC, Chou CC (2006) Antioxidative activities of soymilk fermented with lactic acid bacteria and bifidobacteria. Food Microbiol 23:128–135PubMedCrossRefGoogle Scholar
  26. 26.
    Yao DC, Shi WB, Gou YL, Zhou XR, Tak YA, Zhou YK (2005) Fatty acid mediated intracellular iron translocation: a synergistic mechanism of oxidative injury. Free Radic Bio Med 39:1385–1398CrossRefGoogle Scholar
  27. 27.
    Yasui H, Kiyoshima J, Ushijima H (1995) Passive protection against rotavirus-induced diarrhea of mouse pups born to and nursed by dams fed Bifidobacterium breve YIT4064. J Infect Dis 172:403–409PubMedCrossRefGoogle Scholar
  28. 28.
    Yildirim Z, Johnson MG (1998) Characterization and antimicrobial spectrum of bifidocin B, a bacteriocin produced by Bifidobacterium bifidum NCFB 1454. J Food Protect 61:47–51Google Scholar
  29. 29.
    Zang LY, Cosma G, Gardner H, Vallyathan V (1998) Scavenging of reactive oxygen species by melatonin. Biochim Biophys Acta 1425:469–477PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Key Laboratory of Functional Dairy, College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingPeople’s Republic of China

Personalised recommendations