Current Microbiology

, Volume 62, Issue 4, pp 1097–1103 | Cite as

In Vitro and In Vivo Antioxidant Activity of Bifidobacterium animalis 01 Isolated from Centenarians

Article

Abstract

Several studies reported the antioxidant activity of bifidobacteria using assays in vitro. In present study, the in vitro and in vivo antioxidant activity of Bifidobacterium animalis 01 was investigated. Culture supernatant, intact cells, and intracellular cell-free extracts of B. animalis 01 were involved in this study. The antioxidant assays in vitro included lipid peroxidation assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, hydroxyl radical ( OH) assay and superoxide anion (\( {\text{O}}_{2}^{ - } \)) assay. The antioxidant assays in vivo were conducted using mice model. Activities of antioxidative enzymes, malondialdehyde (MDA) content in serums and livers of aging mice were evaluated. Monoamine oxidase (MAO) activity and lipofuscin level in brains of aging mice were also characterized. Results showed that culture supernatant, intact cells and intracellular cell-free extracts of B. animalis 01 could effectively scavenge free radicals, significantly enhance mice’s activities of antioxidative enzymes and reduce mice’s MDA content, lipofuscin level and MAO activity. Our results indicated that B. animalis 01 has the potential to be developed into a dietary antioxidant supplements.

Keywords

Antioxidant Activity Intact Cell Bifidobacterium Longum Model Control Group Gastric Gavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was funded by China National 863 Program (2008AA10Z324) and Beijing Natural Science Foundation (5072025).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Key Laboratory of Functional Dairy, College of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijingPeople’s Republic of China

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