Current Microbiology

, Volume 62, Issue 3, pp 816–820 | Cite as

Infection Incidence and Relative Density of the Bacteriophage WO-B in Aedes albopictus Mosquitoes from Fields in Thailand

  • Arunee Ahantarig
  • Nopmanee Chauvatcharin
  • Toon Ruang-areerate
  • Visut Baimai
  • Pattamaporn Kittayapong


We have used real-time quantitative PCR to measure, for the first time, the relative phage WO-B orf7 density and infection incidence in Aedes albopictus mosquitoes from fields in Thailand. Our results showed that the infection incidence of phage WO-B in this mosquito, sampled from geographically different places in Thailand, was 97.9%. Average relative densities of the offspring were different when collected from diverse parts and reared under the same conditions in the laboratory. Our results also revealed that geographical differences within Thailand did not influence the maternal transmission rate of bacteriophage WO-B. In addition, the orf7 loci might not be strictly associated with Wolbachia, because less than 100% of them were maternally inherited. This discovery does not support the hypothesis that bacteriophage WO-B is involved in Aedes albopictus’ cytoplasmic incompatibility. Whether this bacteriophage actually is involved in Wolbachia-induced cytoplasmic incompatibility in this mosquito thus needs further investigation, and additional densities of phage WO-B loci should be integrated.


Cytoplasmic Incompatibility Infection Incidence Wolbachia Strain Aedes Albopictus Trichogramma Species 



We are grateful for anonymous reviewer(s) for the advice to improve this article during the review process. We also thank Dr. John R. Milne for reviewing the manuscript; Drs. Ronald Morales Vargas, Supanee Hirunkanokpun, and Supat Wiwatanaratanabutr for their helpful suggestions; and Mr. Kitti Theinthong, Ms. Samnieng Theinthong, and Miss Nutchaya Klinpikul for their technical assistance.


  1. 1.
    Ahantarig A, Khumthong R, Kittayapong P, Baimai V (2008) Relative densities of bacteriophage WO and Wolbachia of Aedes albopictus mosquito during development. Ann Microbiol 58:189–193CrossRefGoogle Scholar
  2. 2.
    Bordenstein S, Marshall ML, Fry AJ, Kim U, Wernegreen JJ (2006) The tripartite associations between bacteriophage, Wolbachia, and arthropods. PLoS Pathogens 2:e43PubMedCrossRefGoogle Scholar
  3. 3.
    Buei K (1983) Pictorial key to species. Adult mosquitoes in Thailand. Ministry of Public Health, BangkokGoogle Scholar
  4. 4.
    Chauvatcharin N, Ahantarig A, Baimai V, Kittayapong P (2006) Bacteriophage WO-B and Wolbachia in natural mosquito hosts: infection incidence, transmission mode and relative density. Mol Ecol 15:2451–2461PubMedCrossRefGoogle Scholar
  5. 5.
    Duron O, Fort P, Weill M (2006) Hypervariable prophage WO sequences describe an unexpected high number of Wolbachia variants in the mosquito Culex pipiens. Proc Biol Sci 273:495–502PubMedCrossRefGoogle Scholar
  6. 6.
    Fujii Y, Kubo T, Ishikawa H, Sasaki T (2004) Isolation and characterization of the bacteriophage WO from Wolbachia, an arthropod endosymbiont. Biochem Biophys Res Commun 317:1183–1188PubMedCrossRefGoogle Scholar
  7. 7.
    Gavotte L, Henri H, Stouthamer R, Charif D, Charlat S, Bouletreau M, Vavre F (2007) A survey of the bacteriophage WO in the endosymbiotic bacteria Wolbachia. Mol Biol Evol 24:427–435PubMedCrossRefGoogle Scholar
  8. 8.
    Guillemaud T, Pasteur N, Rousset F (1997) Contrasting levels of variability between cytoplasmic genomes and incompatibility types in the mosquito Culex pipiens. Proc Biol Sci 264:245–251PubMedCrossRefGoogle Scholar
  9. 9.
    Iturbe-Ormaetxe I, Burke GR, Riegler M, O’Neill SL (2005) Distribution, expression, and motif variability of ankyrin domain genes in Wolbachia pipientis. J Bacteriol 187:5136–5145PubMedCrossRefGoogle Scholar
  10. 10.
    Kambhampati S, Rai KS (1991) Mitochondrial DNA variation within and among populations of the mosquito, Aedes albopictus. Genome 34:288–292PubMedGoogle Scholar
  11. 11.
    Kambhampati S, Black WC, Rai KS (1991) Geographic origin of the US and Brazilian Aedes albopictus inferred from allozyme analysis. Heredity 67:85–94PubMedCrossRefGoogle Scholar
  12. 12.
    Knudsen AB (1995) Global distribution and continuing spread of Aedes albopictus. Parasitologia 37:91–97Google Scholar
  13. 13.
    Masui S, Kamoda S, Sasaki T, Ishikawa H (2000) Distribution and evolution of bacteriophage WO in Wolbachia, the endosymbiont causing sexual alterations in arthropods. J Mol Evol 51:491–497PubMedGoogle Scholar
  14. 14.
    O’Neill SL, Giordane R, Colbert AME, Karr TL, Robertsu HM (1992) 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with CI in insects. Proc Natl Acad Sci USA 89:2699–2702PubMedCrossRefGoogle Scholar
  15. 15.
    Rattanarithikul R, Panthusiri P (1994) Illustrated keys to the medically important mosquitoes of Thailand. Wattana Panich Press, BangkokGoogle Scholar
  16. 16.
    Ruang-areerate T, Kittayapong P (2006) Wolbachia transfection in Aedes aegypti: a potential gene driver of dengue vectors. Proc Natl Acad Sci USA 103:12534–12539PubMedCrossRefGoogle Scholar
  17. 17.
    Sanogo YO, Dobson SL (2004) Molecular discrimination of Wolbachia in the Culex pipiens complex: evidence for variable bacteriophage hyperparasitism. Insect Mol Biol 13:365–369PubMedCrossRefGoogle Scholar
  18. 18.
    Schilthuizen M, Stouthamer R (1997) Horizontal transmission of parthenogenesis-inducing microbes in Trichogramma wasps. Proc R Soc Lond B Sci 264:361–366CrossRefGoogle Scholar
  19. 19.
    Sinkins SP, Walker T, Lynd AR, Steven AR, Makepeace BL, Godfray HC, Parkhill J (2005) Wolbachia variability and host effects on crossing type in Culex mosquitoes. Nature 436:257–260PubMedCrossRefGoogle Scholar
  20. 20.
    Stouthamer R, Breeuwer JA, Hurst GD (1999) Wolbachia pipientis: microbial manipulator of arthropod reproduction. Annu Rev Microbiol 53:71–102PubMedCrossRefGoogle Scholar
  21. 21.
    Werren JH, Windsor DM (2000) Wolbachia infection frequencies in insects: evidence of a global equilibrium? Proc R Soc Lond B 267:1277–1285CrossRefGoogle Scholar
  22. 22.
    Wu M, Sun LV, Vamathevan J, Riegier M, Deboy R et al (2004) Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. PLoS Biol 2:e69. doi: 10.1371/journal.pbio.020069 PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Arunee Ahantarig
    • 1
    • 2
  • Nopmanee Chauvatcharin
    • 1
  • Toon Ruang-areerate
    • 1
    • 3
  • Visut Baimai
    • 1
    • 2
  • Pattamaporn Kittayapong
    • 1
    • 2
  1. 1.Center of Excellence for Vectors and Vector-Borne Diseases, Faculty of ScienceMahidol University at SalayaNakhon PathomThailand
  2. 2.Department of Biology, Faculty of ScienceMahidol UniversityBangkokThailand
  3. 3.Epidemiology Section, Research DivisionArmed Forces Research Institute of Medical SciencesBangkokThailand

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