Advertisement

Current Microbiology

, Volume 62, Issue 2, pp 679–683 | Cite as

Effects of Calcium Levels on Colonial Aggregation and Buoyancy of Microcystis aeruginosa

  • Yu-Wen Wang
  • Jie Zhao
  • Jian-Hong LiEmail author
  • Shan-Shan Li
  • Li-Hua Zhang
  • Min Wu
Article

Abstract

Colonial aggregation of Microcystis plays a key role in bloom formation. Limited studies have been reported about effects of environmental factors on the aggregation of Microcystis. Calcium is an important chemical element in water system. In this study, we investigated the effects of a low- (0.015 g l−1) and a high-concentration of calcium (0.100 g l−1) on the aggregation and buoyancy of a colonial strain M. aeruginosa XW01. Results show that compared to the low concentration of calcium, the high-calcium condition results in bigger colonial size, higher level of buoyancy and increased production of extracellular polysaccharides (EPS) of M. aeruginosa XW01. Increased production of EPS induced by the high-calcium concentration should contribute to the colonial aggregation and buoyancy of M. aeruginosa XW01. These results suggest that an increase in calcium concentration may be beneficial for Microcystis blooms occurring in a soft water lake.

Keywords

Extracellular Polysaccharide Microcystis Bloom High Calcium Level Soft Water Lake Xuanwu Lake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The project was sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and supported by Research Program of Jiangsu Province of China (NO. BS2007065).

References

  1. 1.
    Bittencourt-Oliveira MC (2003) Detection of potential microcystin-producing cyanobacteria in Brazilian reservoirs with a mcyB molecular marker. Harmful Algae 2:51–60CrossRefGoogle Scholar
  2. 2.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  3. 3.
    Brookes J, Ganf GG (2001) Variations in the buoyancy response of Microcystis aeruginosa to nitrogen, phosphorus and light. J Plankton Res 23:1399–1411CrossRefGoogle Scholar
  4. 4.
    Carmichael WW (1994) The toxins of cyanobacteria. Sci Am 270:78–86CrossRefPubMedGoogle Scholar
  5. 5.
    Fulton RS, Paerl HW (1987) Effects of colonial morphology on zooplankton utilisation of algal resources during blue-green algal (Microcystis aeruginosa) blooms. Limnol Oceanogr 32:634–644CrossRefGoogle Scholar
  6. 6.
    Jin XC, Liu HL, Tu QY, Zhang ZS, Zhu X (1990) Eutrophication of lakes in China. China Environmental Science Press, BeijingGoogle Scholar
  7. 7.
    Kim IS, Jang N (2006) The effect of calcium on the membrane biofouling in the membrane bioreactor (MBR). Water Res 40:2756–2764CrossRefPubMedGoogle Scholar
  8. 8.
    Kehr JC, Zilliges Y, Springer A, Disney MD, Ratner DD, Bouchier C, Seeberger PH, Marsac NT, Dittmann E (2006) A mannan binding lectin is involved in cell–cell attachment in a toxic strain of Microcystis aeruginosa. Mol Microbiol 59:893–906CrossRefPubMedGoogle Scholar
  9. 9.
    Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 603:591–592Google Scholar
  10. 10.
    Oliver RL, Ganf GG (2000) Freshwater blooms. In: Whitten BA, Potts M (eds) The ecology of cyanobacteria. Kluwer Acdemic Publishers, Netherlands, pp 149–194Google Scholar
  11. 11.
    Reynolds CS (2007) Variability in the provision and function of mucilage in phytoplankton: facultative responses to the environment. Hydrobiologia 578:3745–3768CrossRefGoogle Scholar
  12. 12.
    Sakamoto S, Yamaguchi M, Watanabe MF, Watanabe M, Kamiya H (1996) Distribution and characterization of lectins from natural and cultured Microcystis spp. In: Yasumoto T, Oshima Y, Fukuyo Y (eds) Harmful and toxic algal blooms. Inter-Government Oceanographic Commission of UNESCO, Paris, pp 569–572Google Scholar
  13. 13.
    Su J, Jia S, Chen X, Yu H (2008) Morphology, cell growth, and polysaccharide production of Nostoc flagelliforme in liquid suspension culture at different agitation rates. J Appl Phycol 20:1573–5176CrossRefGoogle Scholar
  14. 14.
    Waterbury JB (2006) The cyanobacteria—isolation, purification and identification. Prokaryotes 4:1053–1073CrossRefGoogle Scholar
  15. 15.
    Widdel F (2007) Theory and measurement of bacterial growth. Online microbiology notes, http://www.mpi-bremen.de/Binaries/Binary5381/Wachstumsversuch(3).pdf
  16. 16.
    Wilson AE, Wilson WA, Hay ME (2006) Intraspecific variation in growth and morphology of the bloom-forming cyanobacterium Microcystis aeruginosa. Appl Environ Microbiol 11:7386–7389CrossRefGoogle Scholar
  17. 17.
    Wilson AE, Kaul RB, Sarnelle O (2010) Growth rate consequences of coloniality in a harmful phytoplankter. PLoS One 5(1):e8679. doi: 10.1371/journal.pone.0008679(pdf)
  18. 18.
    Ye HM, Yuan XY, Ge MX, Li JH, Sun H (2010) Water chemistry characteristics and controlling factors in the northern rivers in the Taihu Basin. Ecology and Environmental Sciences 19(1):23–27Google Scholar
  19. 19.
    Zhang M, Kong F, Tan X, Yang Z, Cao H, Xing P (2007) Biochemical, morphological, and genetic variations in Microcystis aeruginosa due to colony disaggregation. World J Microbiol Biotechnol 23:663–670CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Yu-Wen Wang
    • 1
  • Jie Zhao
    • 1
  • Jian-Hong Li
    • 1
    Email author
  • Shan-Shan Li
    • 1
  • Li-Hua Zhang
    • 1
  • Min Wu
    • 1
  1. 1.Jiangsu Key Laboratory of Biodiversity and BiotechnologyLife Sciences College, Nanjing Normal UniversityNanjingChina

Personalised recommendations