Current Microbiology

, Volume 62, Issue 2, pp 618–622 | Cite as

Probiotic Lactobacilli Interfere with Streptococcus mutans Biofilm Formation In Vitro

  • Eva M. SöderlingEmail author
  • Aino M. Marttinen
  • Anna L. Haukioja


In clinical studies, probiotic bacteria have decreased the counts of salivary mutans streptococci (MS). We compared the effects of probiotic Lactobacillus strains on the biofilm formation of Streptococcus mutans. The bacterial strains used included four S. mutans strains (reference strains NCTC 10449 and Ingbritt and clinical isolates 2366 and 195) and probiotic strains Lactobacillus rhamnosus GG, L. plantarum 299v, and L. reuteri strains PTA 5289 and SD2112. The ability of MS to adhere and grow on a glass surface, reflecting biofilm formation, was studied in the presence of the lactobacilli (LB). The effect of LB culture supernatants on the viability of the MS was studied as well. All of the LB inhibited the biofilm formation of the clinical isolates of MS (P < 0.001). The biofilm formation of the reference strains of MS was also inhibited by the LB, but L. plantarum and L. reuteri PTA 5289 showed a weaker inhibition when compared to L. reuteri SD2112 and L. rhamnosus GG. Viable S. mutans cells could be detected in the biofilms and culture media only when the experiments were performed with the L. reuteri strains. The L. reuteri strains were less efficient in killing the MS also in the tests performed with the culture supernatants. The pHs of the supernatants of L. reuteri were higher compared to those of L. rhamnosus GG and L. plantarum; P < 0.001. In conclusion, our results demonstrated that four commonly used probiotics interfered with S. mutans biofilm formation in vitro, and that the antimicrobial activity against S. mutans was pH-dependent.


Oral Health Clinical Isolate Mutans Streptococcus Probiotic Lactobacillus Unattached Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The excellent technical assistance of biomedical research technician Oona Hällfors is gratefully acknowledged. Drs Arthur Ouwehand and Anders Zachrisson are acknowledged for providing Dr. Haukioja with the probiotic strains.


  1. 1.
    Banas JA, Vickerman MM (2003) Glucan-binding proteins of the oral streptococci. Crit Rev Oral Biol Med 14:89–99CrossRefPubMedGoogle Scholar
  2. 2.
    Beighton D (2005) The complex oral microflora of high-risk individuals and groups and its role in the caries process. Community Dent Oral Epidemiol 33:248–255CrossRefPubMedGoogle Scholar
  3. 3.
    Caglar E, Kavaloglu S, Ergeneli S, Sandalli N, Twetman S (2006) Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets. Acta Odontol Scand 64:314–318CrossRefPubMedGoogle Scholar
  4. 4.
    Chung J, Ha E-S, Park H-R, Kim S (2004) Isolation and characterization of Lactobacillus species inhibiting the formation of Streptococcus mutans biofilm. Oral Microbiol Immunol 19:214–216CrossRefPubMedGoogle Scholar
  5. 5.
    Hatakka K, Saxelin M (2008) Probiotics in intestinal and non-intestinal infectious diseases–clinical evidence. Curr Pharm Des 14:1351–1367CrossRefPubMedGoogle Scholar
  6. 6.
    Haukioja A, Loimaranta V, Tenovuo J (2008) Probiotic bacteria affect the composition of salivary pellicle and streptococcal adhesion in vitro. Oral Microbiol Immunol 23:336–343CrossRefPubMedGoogle Scholar
  7. 7.
    Haukioja A, Söderling E, Tenovuo J (2008) Acid production from sugars and sugar alcohols by probiotic lactobacilli and bifidobacteria in vitro. Caries Res 42:449–453CrossRefPubMedGoogle Scholar
  8. 8.
    Krasse P, Carlsson B, Dahl C, Paulsson A, Nilsson A, Sinkiewicz G (2006) Decreased gum bleeding and reduced gingivitis by the probiotic Lactobacillus reuteri. Swed Dent J 30:55–60PubMedGoogle Scholar
  9. 9.
    Lang C, Böttner M, Holz C, Veen M, Ryser M, Reindl A, Pompejus M, Tanzer JM (2010) Specific lactobacillus/mutans streptococcus co-aggregation. J Dent Res 89:175–179CrossRefPubMedGoogle Scholar
  10. 10.
    Mattos-Graner RO, Smith DJ, King WF, Mayer MPA (2000) Water-insoluble glucan synthesis by mutans streptococcal strains correlates with caries incidence in 12- to 30-month-old children. J Dent Res 79:1371–1377CrossRefPubMedGoogle Scholar
  11. 11.
    Näse L, Hatakka K, Savilahti E, Saxelin M, Pönkä A, Poussa T, Korpela R, Meurman JH (2001) Effect of long-term consumption of a probiotic bacterium, Lactobacillus rhamnosus GG, in milk on dental caries and caries risk in children. Caries Res 35:412–420CrossRefPubMedGoogle Scholar
  12. 12.
    Nikawa H, Makihira S, Fukushima H, Nishimura H, Ozaki Y, Ishida K, Darmawan S, Hamada T, Hara K, Matsumoto A, Takemoto T, Aimi R (2004) Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci. Int J Food Microbiol 95:219–223CrossRefPubMedGoogle Scholar
  13. 13.
    Silva M, Jacobus N, Deneke C, Gorbach L (1987) Antimicrobial substance from a human Lactobacillus strain. Antimicrob Agents Chemother 31:1231–1233PubMedGoogle Scholar
  14. 14.
    Simark-Mattsson C, Jonsson R, Emilson C-G, Roos K (2009) Final pH affects the interference capacity of naturally occurring oral Lactobacillus strains against mutans streptococci. Arch Oral Biol 54:602–607CrossRefPubMedGoogle Scholar
  15. 15.
    Smaoui S, Elleuch L, Bejar W, Karray-Rebai I, Ayadi I, Jaouadi B, Methieu F, Chouayekh H, Bejar S, Mellouli L (2009) Inhibition of fungi and gram-negative bacteria by bacteriocin BacTN635 produced by Lactobacillus plantarum sp. TN635. Appl Biochem Biotechnol 162(4):1132–1146. doi: 10.1007/s12010-009-8821-7 CrossRefPubMedGoogle Scholar
  16. 16.
    Söderling EM, Hietala-Lenkkeri A (2010) Xylitol and erythritol decrease adherence of polysaccharide-producing oral streptococci. Curr Microbiol 60:25–29CrossRefPubMedGoogle Scholar
  17. 17.
    Spinler J, Taweechotipar M, Rognerud C, Ou C, Tumwasorn S, Versalovic J (2008) Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe 14:166–171CrossRefPubMedGoogle Scholar
  18. 18.
    Stecksen-Blicks C, Sjöström I, Twetman S (2009) Effect of long-term consumption of milk supplemented with probiotic lactobacilli and fluoride on dental caries and general health in preschool children: a cluster-randomized study. Caries Res 43:374–381CrossRefPubMedGoogle Scholar
  19. 19.
    Tanzer JM, Livingston J, Thompson AM (2001) The microbiology of primary dental caries in humans. J Dent Educ 65:1028–1037PubMedGoogle Scholar
  20. 20.
    Twetman L, Larsen U, Fiehn N-E, Stecksen-Blicks C, Twetman S (2009) Coaggregation between probiotic bacteria and caries-associated strains: an in vitro study. Acta Odontol Scand 67:284–288CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Eva M. Söderling
    • 1
    Email author
  • Aino M. Marttinen
    • 1
  • Anna L. Haukioja
    • 1
  1. 1.Institute of DentistryUniversity of TurkuTurkuFinland

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