Current Microbiology

, Volume 62, Issue 2, pp 606–611 | Cite as

Joostella atrarenae sp. nov., a novel member of the Flavobacteriaceae originating from the black sea sand of Jeju Island

  • Byung-Chun Kim
  • Kang Hyun Lee
  • Mi Na Kim
  • Min Young Jung
  • Young-Hyo Chang
  • Jongtae Lee
  • Kee-Sun Shin


Strain M1-2T was isolated from the black sand from the seashore of Jeju Island, Republic of Korea and was classified using a polyphasic taxonomic approach. Strain M1-2T appeared as Gram-negative, motile rods that could grow in the presence of 1–10% (w/v) NaCl and at temperatures ranging from 4 to 37°C. This isolate has catalase and oxidase activity and hydrolyses aesculin, DNA and l-tyrosine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain M1-2T belongs to the genus Joostella and is clearly distinct from the other described species of this genus, Joostella marina (type strain En5T). The 16S rRNA gene sequence similarity level between M1-2T and J. marina En5T is 97.2%, and the DNA–DNA relatedness value between the two strains is 23.9%. Strain M1-2T contains MK-6 as the major menaquinone and iso-C15:0, summed feature 3 (C16:1 ω7c and/or iso-C15:0 2OH) and iso-C17:0 3OH as major cellular fatty acids. The DNA G + C content is 32.3 mol%. These data suggest that strain M1-2T should be classified as a novel species, for which the name Joostella atrarenae sp. nov. is proposed. The type strain for the novel species is M1-2T (= KCTC 23194T = NCAIM B.002413T).


Isoprenoid Quinone Major Cellular Fatty Acid Major Respiratory Quinone Carbon Source Utilisation Profile Heterotrophic Bacterial Diversity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Dr. Hans Georg Trüper for his advice on the Latin naming of the organism. This work was supported by the Mid-career Researcher Program through an NRF Grant funded by the MEST (No. 2009-0083557), Grant NMC0301039 and a Grant from the KRIBB Research Initiative Program.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Byung-Chun Kim
    • 1
  • Kang Hyun Lee
    • 1
  • Mi Na Kim
    • 1
  • Min Young Jung
    • 1
  • Young-Hyo Chang
    • 1
  • Jongtae Lee
    • 2
  • Kee-Sun Shin
    • 1
  1. 1.Biological Resource CenterKorea Research Institute of Bioscience & BiotechnologyDaejeonKorea
  2. 2.Product Technology Part, Central Research InstituteKorea Ginseng Corp.DaejeonKorea

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