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Current Microbiology

, Volume 62, Issue 2, pp 539–543 | Cite as

Photorhabdus luminescens subsp. kleinii subsp. nov. (Enterobacteriales: Enterobacteriaceae)

  • Ruisheng An
  • Parwinder S. Grewal
Article

Abstract

Association between bacteria Photorhabdus and their nematode hosts Heterorhabditis represents one of the emerging models in symbiosis studies. In this study, we isolated the bacterial symbionts of the nematode Heterorhabditis georgiana. Using gyrB sequences for phylogenetic analysis, these strains were shown to be part of the species of Photorhbdus luminescens but with clear separation from currently recognized subspecies. Physiological properties and DNA–DNA hybridization profiles also supported the phylogenetic relationship of these strains. Therefore, a new subspecies, Photorhabdus luminescens subsp. kleinii subsp. nov., is proposed with the type strain KMD37T (=DSM 23513 =ATCC =NRRL B-59419).

Keywords

Symbiotic Bacterium Infective Juvenile Bromothymol Blue gyrB Gene Sequence Enterobacteriales 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by a grant from the Ohio Agricultural Research and Development Center of The Ohio State University, Wooster, Ohio. The authors thank Drs. Byron J. Adams and Michael D. Klein for providing nematode strains.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Entomology, OARDCThe Ohio State UniversityWoosterUSA

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