Current Microbiology

, Volume 62, Issue 2, pp 391–395 | Cite as

Field Evidence for the Potential of Rhodobacter capsulatus as Biofertilizer for Flooded Rice

  • Hosny Gamal-Eldin
  • Khaled ElbannaEmail author


In a previous study, we evaluated the effects of inoculating rice plants with the phototrophic purple nonsulfur bacterium Rhodobacter capsulatus (Rc) on growth and yield of rice in pots and lysimeter experiments and the results obtained have been highly encouraging. In this study, we carried out two field experiments: one in the experimental farm of the Faculty of Agriculture, Fayoum University, and the second in a farmer’s field in Kafr El-sheikh, to assess the effects of Rc on growth and yield of rice in comparison and in combination with chemical nitrogen fertilizer (CNF) and farmyard manure. The results indicated that both biological and grain yields in all the Rc inoculated treatments were significantly higher than those in the uninoculated corresponding treatments in both fields. With regard to grain yield, the major factor for determining the effectiveness of any agricultural treatment, inoculation with Rc in combination with 50% of the recommended CNF rate gave a grain yield that was statistically equivalent to that obtained with 100% of the recommended CNF rate. These results provide a clear evidence for the potential of Rc as biofertilizer for flooded rice under field conditions.


Rice Plant Rice Straw Rice Cultivation Biological Nitrogen Fixation Rhodobacter Capsulatus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors would like to thank Dr. Martin Krehenbrink (Oxford University, UK) for helpful revision.


  1. 1.
    Choudhury AT, Kennedy IR (2004) Prospects and potentials for systems of biological nitrogen fixation in sustainable rice production. Biol Fertil Soils 39:219–227CrossRefGoogle Scholar
  2. 2.
    Elbadry M, Elbanna K (1999) Response of four rice varieties to Rhodobacter capsulatus at seedling stage. World J Microbiol Biotechnol 15:363–367CrossRefGoogle Scholar
  3. 3.
    Elbadry M, Gamal-Eldin H, Elbanna K (1999) Effects of Rhodobacter capsulatus inoculation in combination with graded levels of nitrogen fertilizer on growth and yield of rice in pots and lysimeter experiments. World J Microbiol Biotechnol 15:393–395CrossRefGoogle Scholar
  4. 4.
    Freitez JR, Germida JJ (1992) Growth promotion of winter wheat by fluorescent pseudomonas under growth chamber conditions. Soil Biol Biochem 24:1127–1135CrossRefGoogle Scholar
  5. 5.
    Habte M, Alexander M (1980) Nitrogen fixation by photosynthetic bacteria in lowland rice culture. Appl Environ Microbiol 38:342–347Google Scholar
  6. 6.
    Harada N, Nishiyama M, Otsuka S, Mastumoto S (2005) Effects of inoculation of phototrophic purple bacteria on grain yield of rice and nitrogenase activity of paddy soil in a pot experiment. Soil Sci Plant Nutr 51:361–367CrossRefGoogle Scholar
  7. 7.
    IRRI (International Rice Research Institute) (2003) World rice statistics.
  8. 8.
    Kobayashi M, Haque, MZ (1971) Contribution to nitrogen fixation and soil fertility by photosynthetic bacteria. Plant Soil (Special Volume):443–456Google Scholar
  9. 9.
    Koh RH, Song HG (2007) Effects of application of Rhodopseudomonas sp. on seed germination and growth of tomato under axenic conditions. J Microbiol Biotechnol 17:1805–1810PubMedGoogle Scholar
  10. 10.
    Kondo K, Nakata N, Nishihara E (2004) Effect of purple nonsulfur bacteria (Rhodobacter sphaeroides) on the growth and quality of Komatsuna under different light qualities. Environ Control Biol 42(3):247–253Google Scholar
  11. 11.
    Kondo K, Nakata N, Nishihara E (2006). Effect of the purple nonsulfur bacterium Rhodobacter sphaeroides on fruit quality of tomato. In: xxxII International Horticultural Congress—IHC 2006Google Scholar
  12. 12.
    Ladha JK, Tirol-Pande A, Punzalan GC, Watanabe I (1987) Nitrogen-fixing (C2H2-reducing) activity and plant growth characters of 16 wetland rice varieties. Soil Sci Plant Nutr 33:187–200Google Scholar
  13. 13.
    Ladha JK, Tirol-Padre A, Reddy K, Ventura W (1993) Prospects and problems of biological nitrogen fixation in rice production. In: Palacios R et al (eds) New horizons in nitrogen fixation. Kluwer Academic Publishers, Dordrecht, pp 677–682Google Scholar
  14. 14.
    Lee KH, Koh RH, Song HG (2008) Enhancement of growth and yield of tomato by Rhodopseudomonas sp. under greenhouse conditions. J Microbiol 46:641–646CrossRefPubMedGoogle Scholar
  15. 15.
    Maudinas B, Chemardin M, Yovanovitch E, Gadal P (1981) Gnotobiotic culture of rice plants up to ear stage in the absence of combined nitrogen source but in the presence of free living nitrogen fixing bacteria Azotobacter vinelandii and Rhodopseudomonas capsulatus. Plant Soil 60:85–97CrossRefGoogle Scholar
  16. 16.
    Montano GL, Chan JS, Jarabelo RE, Pastor AB, Delacruz TE (2009) Isolation and characterization of purple nonsulfur bacteria (PNSB) from a rice paddy soil in Bulacan, Philippines. Philipp J Syst 3:57–67Google Scholar
  17. 17.
    Ranjith NK, Sasikala Ch, Ramana Ch (2007) Rhodethrin: a novel indol terpenoid ether has cytotoxic and phytohormonal activity by Rhodobacter sphaeroides OU5. Biotechnol Lett 26:1399–1402CrossRefGoogle Scholar
  18. 18.
    Roger PA, Ladha JK (1992) Biological nitrogen fixation in wetland rice fields: estimation and contribution to nitrogen balance. Plant Soil 141:41–45CrossRefGoogle Scholar
  19. 19.
    Serdyuk OP, Smolygiana LD, Kobzar EF, Gogotov IN (1993) Occurrence of plant hormones in cells of the phototrophic purple bacterium Rhodospirillum rubrum 1R. FEMS Microbiol Lett 109:113–116CrossRefGoogle Scholar
  20. 20.
    Tsavkelova EA, Klimova SY, Cherdyntseva TA, Netrusov AI (2006) Microbial producers of plant growth stimulators and their practical use: a review. Appl Biochem Microbiol 42:117–126CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Agricultural MicrobiologyFayoum UniversityFayoumEgypt

Personalised recommendations