Overexpression of Rusticyanin in Acidithiobacillus ferrooxidans ATCC19859 Increased Fe(II) Oxidation Activity
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A wide-host-range plasmid pTRUS containing a homologous rus gene under the control of Ptac promoter was constructed and transferred into Acidithiobacillus ferrooxidans ATCC19859 using conjugation gene transfer method to generate the engineered strain of A. ferrooxidans(pTRUS). The plasmid-based recombinant rus gene was successfully expressed. According to the results of real-time quantitative PCR assay, not only the transcription level of recombinant rus gene but also the transcription levels of the other genes of rus operon (cyc1, orf, coxB, coxA) were increased in A. ferrooxidans(pTRUS). The ferrous ion (Fe2+) oxidation activity was increased in A. ferrooxidans(pTRUS).
KeywordsOxidation Activity Plasmid Stability Acidithiobacillus Ferrooxidans Blue Copper Filter Mating
This study was supported by the Chinese National Basic Research Program (2004CB619202, 2010CB630902), the National High Technology Research and Development Program (2007AA06A407), and the Knowledge Innovation Program of CAS (2AKSCX2-YW-JS401). The authors thank Dr. Roberta Greenwood for her help in editing this manuscript.
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