Advertisement

Current Microbiology

, Volume 62, Issue 1, pp 229–234 | Cite as

Oxidative Damage Involves in the Inhibitory Effect of Nitric Oxide on Spore Germination of Penicillium expansum

  • Tongfei Lai
  • Boqiang Li
  • Guozheng Qin
  • Shiping TianEmail author
Article

Abstract

The effects of nitric oxide (NO) on spore germination of Penicillium expansum were investigated and a possible mechanism was evaluated. The results indicated that NO released by sodium nitroprusside (SNP) significantly suppressed fungal growth. With the use of an oxidant sensitive probe and Western blot analysis, an increased level of intracellular reactive oxygen species (ROS) and enhanced carbonylation damage were detected in spores of P. expansum under NO stress. Exogenous superoxide dismutase (SOD) and ascorbic acid (Vc) could increase the resistance of the spore to the inhibitory effect of NO. The activities of SOD and catalase (CAT), as well as ATP content in spores under NO stress were also lower than those in the control. We suggest that NO in high concentration induces the generation of ROS which subsequently causes severe oxidative damage to proteins crucial to the process of spore germination of P. expansum.

Keywords

Reactive Oxygen Species Nitric Oxide Carbonylated Protein Spore Germination Intracellular Reactive Oxygen Species Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by National Basic Research Program of China (973 Program) Grant (2006CB1019007), the National Natural Science Foundation of China (30972069), and the Ministry of Science and Technology of China (2006BAD22B02).

References

  1. 1.
    Beltrán B, Mathur A, Duchen MR et al (2000) The effect of nitric oxide on cell respiration: a key to understanding its role in cell survival or death. Proc Natl Acad Sci USA 97:14602–14617CrossRefPubMedGoogle Scholar
  2. 2.
    Brown GC (1995) Reversible binding and inhibition of catalase by nitric oxide. Eur J Biochem 232:188–191CrossRefPubMedGoogle Scholar
  3. 3.
    Carreras MC, Franco MC, Peralta JG et al (2004) Nitric oxide, complex I, and the modulation of mitochondrial reactive species in biology and disease. Mol Aspects Med 25:125–139CrossRefPubMedGoogle Scholar
  4. 4.
    Carreras MC, Poderoso JJ (2008) Mitochondrial nitric oxide in the signaling of cell integrated responses. Am J Physiol Cell Physiol 292:1569–1580CrossRefGoogle Scholar
  5. 5.
    Chen CB, Dickman MB (2005) Proline suppresses apoptosis in the fungal pathogen Colletotrichum trifolii. Proc Natl Acad Sci USA 102:3459–3462CrossRefPubMedGoogle Scholar
  6. 6.
    Fang FC (1997) Mechanism of nitric oxide-related antimicrobial activity. J Clin Invest 99:2818–2825CrossRefPubMedGoogle Scholar
  7. 7.
    Giulivi C, Kato K, Cooper CE (2006) Nitric oxide regulation of mitochondrial oxygen consumption I: cellular physiology. Am J Physiol Cell Physiol 291:1225–1231CrossRefGoogle Scholar
  8. 8.
    Lazar EE, Wills RBH, Ho BT et al (2008) Antifungal effect of gaseous nitric oxide on mycelium growth, sporulation and spore germination of the postharvest horticulture pathogens, Aspergillus niger, Monilinia fructicola and Penicillium italicum. Lett Appl Microbiol 46:688–692CrossRefPubMedGoogle Scholar
  9. 9.
    Marek P, Annamalai T, Venkitanarayanan K (2003) Detection of Penicillium expansum by polymerase chain reaction. Int J Food Microbiol 89:139–144CrossRefPubMedGoogle Scholar
  10. 10.
    Mur LAJ, Carver TLW, Prats E (2006) NO way to live; the various role of nitric oxide in plant-pathogen interactions. J Exp Bot 57:489–502CrossRefPubMedGoogle Scholar
  11. 11.
    Nisoli E, Carruba MO (2006) Nirtic oxide and mitochondrial biogenesis. J Cell Sci 119:2855–2862CrossRefPubMedGoogle Scholar
  12. 12.
    Qin GZ, Tian SP (2005) Enhancement of biocontrol activity of Cryptococcus laurentii by silicon and possible mechanisms involved. Phytopathology 1:69–75CrossRefGoogle Scholar
  13. 13.
    Qin GZ, Tian SP, Chan ZL et al (2007) Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum. Mol Cell Proteomics 14:425–438Google Scholar
  14. 14.
    Stewart VC, Sharpe MA, Clark JB et al (2000) Astrocyte-derived nitric oxide cause both reversible and irreversible damage to the neuronal mitochondrial respiratory chain. J Neurochem 75:694–699CrossRefPubMedGoogle Scholar
  15. 15.
    Thomas DD, Ridnour LA, Isenberg JS et al (2008) The chemical biology of nitric oxide: implications in cellular signaling. Free Radical Bio Med 45:18–31CrossRefGoogle Scholar
  16. 16.
    Tian SP, Yao HJ, Deng X et al (2007) Characterization and expression of β-1,3-glucanase genes in jujube fruit induced by the microbial biocontrol agent Cryptococcus laurentii. Phytopathology 3:261–268Google Scholar
  17. 17.
    Wang J, Higgins VJ (2005) Nitric oxide has a regulatory effect on the germination of conidia of Colletotrichum coccodes. Fungal Genet Biol 42:284–292CrossRefPubMedGoogle Scholar
  18. 18.
    Wang Y, Tian SP, Xu Y (2005) Effects of high oxygen concentration on pro- and anti-oxidant enzymes in peach fruits during postharvest periods. Food Chem 91:99–104CrossRefGoogle Scholar
  19. 19.
    Wills RBH, Ku VVV, Leshem YY (2000) Fumigation with nitric oxide to extend the postharvest life of strawberries. Postharvest Biol Technol 19:75–79CrossRefGoogle Scholar
  20. 20.
    Wink DA, Hanbauer I, Grisham MB et al (1996) Chemical biology of nitric oxide: regulation and protective and toxic mechanisms. Curr Top Cell Regul 34:159–187CrossRefPubMedGoogle Scholar
  21. 21.
    Wink DA, Mitchell JB (1998) Chemical biology of nitric oxide: insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med 25:434–456CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Tongfei Lai
    • 1
    • 2
  • Boqiang Li
    • 1
  • Guozheng Qin
    • 1
  • Shiping Tian
    • 1
    Email author
  1. 1.Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of SciencesBeijingChina
  2. 2.Graduate School of Chinese Academy of SciencesBeijingChina

Personalised recommendations