Quorum Sensing in Aeromonas Species Isolated from Patients in Malaysia
Bacterial quorum sensing signal molecules called N-acylhomoserine lactone (AHL) controls the expression of virulence determinants in many Gram-negative bacteria. We determined AHL production in 22 Aeromonas strains isolated from various infected sites from patients (bile, blood, peritoneal fluid, pus, stool and urine). All isolates produced the two principal AHLs, N-butanoylhomoserine lactone (C4-HSL) and N-hexanoyl homoserine lactone (C6-HSL). Ten isolates also produced additional AHLs. This report is the first documentation of Aeromonas sobria producing C6-HSL and two additional AHLs with N-acyl side chain longer than C6. Our data provides a better understanding of the mechanism(s) of this environmental bacterium emerging as a human pathogen.
KeywordsQuorum Sense Aeromonas Thin Layer Chromatography Plate Violacein Aeromonas Species
This work was supported by the Malaysia Ministry of Science, Technology and Innovation under the Science Fund (02-01-03-SF0494) to KGC; and two grants from the University of Malaya namely PJP (Vot F) Research Grant (FS260/2008C) to CSW, and Research University Grant (RG003/09BIO) to KGC, which are all gratefully acknowledged.
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