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Current Microbiology

, Volume 62, Issue 1, pp 167–172 | Cite as

Quorum Sensing in Aeromonas Species Isolated from Patients in Malaysia

  • Kok-Gan Chan
  • Savithri D. Puthucheary
  • Xin-Yue Chan
  • Wai-Fong Yin
  • Cheng-Siang Wong
  • Wah-Seng See Too
  • Kek-Heng Chua
Article

Abstract

Bacterial quorum sensing signal molecules called N-acylhomoserine lactone (AHL) controls the expression of virulence determinants in many Gram-negative bacteria. We determined AHL production in 22 Aeromonas strains isolated from various infected sites from patients (bile, blood, peritoneal fluid, pus, stool and urine). All isolates produced the two principal AHLs, N-butanoylhomoserine lactone (C4-HSL) and N-hexanoyl homoserine lactone (C6-HSL). Ten isolates also produced additional AHLs. This report is the first documentation of Aeromonas sobria producing C6-HSL and two additional AHLs with N-acyl side chain longer than C6. Our data provides a better understanding of the mechanism(s) of this environmental bacterium emerging as a human pathogen.

Keywords

Quorum Sense Aeromonas Thin Layer Chromatography Plate Violacein Aeromonas Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was supported by the Malaysia Ministry of Science, Technology and Innovation under the Science Fund (02-01-03-SF0494) to KGC; and two grants from the University of Malaya namely PJP (Vot F) Research Grant (FS260/2008C) to CSW, and Research University Grant (RG003/09BIO) to KGC, which are all gratefully acknowledged.

References

  1. 1.
    Anguita J, Rodriguez-Aparicio LB, Naharro G (1993) Purification, gene cloning, amino acid sequence analysis, and expression of an extracellular lipase from an Aeromonas hydrophila human isolate. Appl Environ Microbiol 59:2411–2417PubMedGoogle Scholar
  2. 2.
    Chan KG (2009) Exochelin production in Mycobacterium neoaurum. Int J Mol Sci 10:345–353CrossRefPubMedGoogle Scholar
  3. 3.
    Eberl L (2006) Quorum sensing in the genus Burkholderia. Int J Med Microbiol 296:103–110CrossRefPubMedGoogle Scholar
  4. 4.
    Kamlesh J, Kong R, Patole MS et al (2007) LuxRI homologs are universally present in the genus Aeromonas. BMC Microbiol 7:93CrossRefGoogle Scholar
  5. 5.
    Lee WS, Puthucheary SD (2001) Retrospective study of Aeromonas infection in a Malaysian Urban Area: a 10-year experience. Singap Med J 42:57–60Google Scholar
  6. 6.
    Leung KY, Stevenson RM (1988) Tn5-induced protease-deficient strains of Aeromonas hydrophila with reduced virulence for fish. Infect Immun 56:2639–2644PubMedGoogle Scholar
  7. 7.
    Lynch MJ, Swift S, Kirke DF et al (2002) The regulation of biofilm development by quorum sensing in Aeromonas hydrophila. Environ Microbiol 4:18–28CrossRefPubMedGoogle Scholar
  8. 8.
    McClean KH, Winson MK, Fish L et al (1997) Quorum sensing and Chromobacterium violaceum: exploitation of violacein production for the detection of N-acylhomoserine lactones. Microbiology 143:3703–3711CrossRefPubMedGoogle Scholar
  9. 9.
    Medina-Martíneza MS, Uyttendaele M, Demolder V et al (2006) Influence of food system conditions on N-acyl homoserine lactones production by Aeromonas spp. Int J Food Microbiol 112:244–252CrossRefGoogle Scholar
  10. 10.
    Merino S, Rubires X, Knøchel S et al (1995) Emerging pathogens: Aeromonas spp. Int J Food Microbiol 28:157–168CrossRefPubMedGoogle Scholar
  11. 11.
    Namdari H (1991) Aeromonas species: pathogens of aquatic inhabitants with a human host range. Clin Microbiol Newslett 15:113–116CrossRefGoogle Scholar
  12. 12.
    Noonan B, Trust TJ (1997) The synthesis, secretion and role in virulence of the paracrystalline surface protein layers of Aeromonas salmonicida and A. hydrophila. FEMS Microbiol Lett 154:1–7CrossRefPubMedGoogle Scholar
  13. 13.
    Pemberton JM, Kidd SP, Schmidt R (1997) Secreted enzymes of Aeromonas. FEMS Microbiol Lett 152:1–10CrossRefPubMedGoogle Scholar
  14. 14.
    Smith RS, Iglewski BH (2003) P. aeruginosa quorum-sensing systems and virulence. Curr Opin Microbiol 6:56–60CrossRefPubMedGoogle Scholar
  15. 15.
    Swift S, Karlyshev AV, Fish L et al (1997) Quorum sensing in Aeromonas hydrophila and Aeromonas salmonicida: identification of the luxRI homologues AhyRI and AsaRI and their cognate N-acylhomoserine lactone signal molecules. J Bacteriol 179:5271–5281PubMedGoogle Scholar
  16. 16.
    Swift S, Lynch MJ, Fish L et al (1999) Quorum sensing-dependent regulation and blockade of exoprotease production in Aeromonas hydrophila. Infect Immun 67:5192–5199PubMedGoogle Scholar
  17. 17.
    Thornley JP, Shaw JG, Gryllos IA et al (1997) Virulence properties of clinically significant Aeromonas species: evidence for pathogenicity. Rev Med Microbiol 8:61–72Google Scholar
  18. 18.
    Vadivelu J, Puthucheary SD, Parasakthi N (1991) Exotoxin profiles of clinical isolates of Aeromonas hydrophila. J Med Microbiol 35:363–367CrossRefGoogle Scholar
  19. 19.
    Vadivelu J, Puthucheary SD, Phipps M et al (1995) Possible virulence factors involved in bacteraemia caused by Aeromonas hydrophila. J Med Microbiol 42:171–174CrossRefPubMedGoogle Scholar
  20. 20.
    Venturi V, Friscina A, Bertani I et al (2004) Quorum sensing in Burkholderia cepacia complex. Res Microbiol 155:238–244CrossRefPubMedGoogle Scholar
  21. 21.
    Whitehead NA, Barnard AM, Slater H et al (2001) Quorum-sensing in Gram-negative bacteria. FEMS Microbiol Rev 25:365–404CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Kok-Gan Chan
    • 1
  • Savithri D. Puthucheary
    • 2
  • Xin-Yue Chan
    • 1
  • Wai-Fong Yin
    • 1
  • Cheng-Siang Wong
    • 1
  • Wah-Seng See Too
    • 1
  • Kek-Heng Chua
    • 3
  1. 1.Institute of Biological Sciences (Division of Genetics and Molecular Biology), Faculty of ScienceUniversity of MalayaKuala LumpurMalaysia
  2. 2.Department of Medical MicrobiologyUniversity of MalayaKuala LumpurMalaysia
  3. 3.Department of Molecular Medicine, Faculty of MedicineUniversity of MalayaKuala LumpurMalaysia

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