Composition of Bacterial Communities Associated with a Plant–Parasitic Nematode Bursaphelenchus mucronatus
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Bursaphelenchus mucronatus is a plant–parasitic nematode widely existing in Eurasian pine forests. To analyze the diversity and role of bacteria associated with the nematode, culture-dependent and culture-independent methods were used to identify and characterize the composition of bacterial community. A total of 13 bacterial isolates were obtained from B. mucronatus by the culture-dependent method. Sixty-four species of bacteria were identified from two 16S rDNA clone libraries constructed from the nematodes of a Chinese and a Japanese population. These bacteria were clustered into four groups: Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Bacteroidetes. Comparison of the two libraries showed that the Chinese library had a higher diversity than that of the Japanese library, and the dominant group and species in each library were also different. In the Japanese library, Alphaproteobacteria group was obviously dominant (60.3%), and Rhizobium sp. was the most dominant species. Whereas in the Chinese library the proportion of each group was similar (from 19.4 to 23.6%), and Pedobacter sp. was a slightly dominant species. Moreover, 18 operational taxonomic units (OTUs) were obtained from each of the two libraries according to a 97% sequence similarity. Metabolic analysis showed that 61.5 and 38.5% of the bacterial isolates could have protease and lipase activities, respectively. But only one had cellulase activity. Testing of reproductive parameter showed that the wild-type nematodes (bacteria carried) could produce more progeny than the bacterium-free nematodes did. So, we speculated that bacteria could promote the propagation and development of the nematode B. mucronatus.
KeywordsBacterial Community Bacteroidetes Gammaproteobacteria Parasitic Nematode Alphaproteobacteria
This study was supported by the National Basic Research and Development Plan (2009CB119200). We are very grateful to John R. Shorter and Lauren E. Brierley for English-language editing. We also thank to Xia Yan for samples collection and Dr. Heng Jian for donating bacterium-free nematodes. This study was supported by the National Basic Research and Development Plan (2009CB119200).
- 2.Braasch H (1996) Pathogenitätstests mit Bursaphelenchus mucronatus an Kiefernund Fichtensämlingen in Deutschland Eruopean. J For Pathol 26:205–216Google Scholar
- 8.Iwahori H, Kanzaki N, Futai K (2002) Bursaphelenchus xylophilus and B. mucronatus in Japan: where are they from? In: The fourth international congress of nematology, SpainGoogle Scholar
- 10.Kawazu K, Kaneko N, Hiraoka K et al (1999) Reisolation of the pathogens from wilted red pine seedlings inoculated with the bacterium carrying nematode, and the cause of difference in pathogenicity among pine wood nematode isolates. Sci Rep Fac Agric Okayama Univ 88:1–5Google Scholar
- 11.Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–175Google Scholar
- 12.Lin F, Zhao BG (2006) Effects of bacterium on the propagation of pine wood nematodes. J Beijing For Univ 28:135–138Google Scholar
- 13.Lin F, Zhao BG (2005) Effects of pine wood nematode on propagation of its carrying bacteria. Chin J Appl Ecol 16:24–76Google Scholar
- 20.Süleyman A, Beflir Y, Metin S et al (2007) Pathogenicity of Bursaphelenchus mucronatus in Pine seedlings under greenhouse conditions. Turk J Agric For 31:169–173Google Scholar
- 25.Webster JM, Anderson V (1990) DNA probes for differentiating isolates of the pinewood nematode species complex. Rev Nematol 13:255–263Google Scholar
- 28.Zhao BG, Liu YT, Lin F (2005) Mutual influences between Bursaphelenchus xylophilus and bacteria carries. J Nanjing For Univ 29:1–4Google Scholar