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Current Microbiology

, Volume 62, Issue 1, pp 101–110 | Cite as

LasR Receptor for Detection of Long-Chain Quorum-Sensing Signals: Identification of N-Acyl-homoserine Lactones Encoded by the avsI Locus of Agrobacterium vitis

  • Michael A. SavkaEmail author
  • Phuong T. Le
  • Thomas J. Burr
Article

Abstract

Bacterial biosensor strains have greatly facilitated the rapid discovery, isolation, and study of quorum-sensing systems. In this study, we determined the relative sensitivity of a LasR-based E. coli bacterial bioluminescence biosensor JM109 (pSB1075) for 13 diverse long-chain N-acyl-homoserine lactones (AHLs) including oxygen-substituted and -unsubstituted AHLs containing 14, 16, and 18 carbons and with and without double bonds. Furthermore, we show by bioassay, HPLC, and GC/MS that four long-chain AHLs of the C16-HSL family are encoded by the avsI gene of Agrobacterium vitis strain F2/5, a non-tumorigenic strain that inhibits pathogenic strains of A. vitis from causing crown gall on grape. The four C16-HSLs include: C16-HSL, N-hexadecanoyl homoserine lactone; 3-oxo-C16-HSL, N-(3-oxohexadecanoyl)homoserine lactone; C16:1-HSL, N-(cis-9-octadecenoyl)homoserine lactone; and 3-oxo-C16:1-HSL, N-(3-oxo-cis-11-hexadecenoyl)homoserine lactone. Thus, the LasR-based bioluminescent biosensor tested in this study should serve as a useful tool for the detection of various long-chain AHLs with and without double bonds as well as those oxylated at the third carbon from uninvestigated species.

Keywords

High Pressure Liquid Chromatography Quorum Sense Homoserine Homoserine Lactone Acyl Side Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

M. A. Savka thanks Rochester Institute of Technology (RIT) for a sabbatical leave of absence. This study was supported by the NRI Competitive Grants Program/USDA award numbers 2002-35319-12577 to M. A. Savka and 2006-35319-16558 to T. J .Burr.

Supplementary material

284_2010_9679_MOESM1_ESM.pdf (40 kb)
Supplementary material 1 (PDF 40 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Michael A. Savka
    • 1
    Email author
  • Phuong T. Le
    • 1
  • Thomas J. Burr
    • 2
  1. 1.School of Biological and Medical SciencesRochester Institute of TechnologyRochesterUSA
  2. 2.Department of Microbial Biology and Plant PathologyCornell UniversityGenevaUSA

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