Current Microbiology

, Volume 62, Issue 1, pp 44–54 | Cite as

Morphological and Molecular Differences in Two Strains of Ustilago esculenta

  • Wenyu You
  • Qian Liu
  • Keqin Zou
  • Xiaoping Yu
  • Haifeng Cui
  • Zihong Ye
Article

Abstract

Ustilago esculenta is a fungal endophyte of Zizania latifolia that plays an important agricultural role in this vegetable crop. The purpose of this study was to characterize sporidial (T) and mycelial (M-T) strains of U. esculenta isolated from sporulating and non-sporulating galls on plants growing in Zhejiang province, China. Morphological comparisons of the T strain and M-T strain were made by optical and scanning electron microscope observation. Genetic differences were examined by sequencing the ITS region of the fungus and examining differential protein expression by two-dimensional gel electrophoresis and MALDI-TOF-MS/MS. The sporidial (T) and mycelial (M-T) strains differed in morphological characteristics of their in vitro single colony formations and in cell shape. Alignment of ITS sequences of the T strain and M-T strain revealed a single mutation between the T strain and M-T strain, but the sequences were the same within strains. A total of 146 proteins were only expressed in the M-T strain, and 242 proteins were only expressed in the T strain isolated from infected plants. A total of 222 proteins were up-regulated or down-regulated in the T strain when compared with the M-T strain. Of these, 18 proteins were identified and eight were associated with processes involving energy metabolism and the cytoskeleton. Two morphology-related proteins, MAP kinase kinase and actin, were differentially expressed. The differences noted in the T strain and M-T strain may lead to a better understanding of the life cycle and morphogenesis in U. esculenta.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Wenyu You
    • 1
  • Qian Liu
    • 1
  • Keqin Zou
    • 1
  • Xiaoping Yu
    • 1
  • Haifeng Cui
    • 1
  • Zihong Ye
    • 1
    • 2
  1. 1.Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life SciencesChina Jiliang UniversityHangzhouChina
  2. 2.Department of Biotechnology, College of Life SciencesChina Jiliang UniversityHangzhouChina

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