Current Microbiology

, Volume 60, Issue 6, pp 446–453

Characteristics of Biofilms from Urinary Tract Catheters and Presence of Biofilm-Related Components in Escherichia coli

  • Xiaoda Wang
  • Heinrich Lünsdorf
  • Ingrid Ehrén
  • Annelie Brauner
  • Ute Römling
Article

DOI: 10.1007/s00284-009-9563-z

Cite this article as:
Wang, X., Lünsdorf, H., Ehrén, I. et al. Curr Microbiol (2010) 60: 446. doi:10.1007/s00284-009-9563-z

Abstract

Long term catheterization of the urinary tract leads to bacterial colonization of the urine, whereby adherence to the catheter surface is a major determinative factor for colonization. Collection of bacterial isolates from urine and urinary catheters of 45 patients showed multi-species catheter-colonization, while Escherichia coli isolates were frequently found in the urine in high numbers. Biofilm formation of catheter and urine-derived E. coli isolates was associated with the presence of the fluA gene, loss of O-antigen, and expression of type 1 fimbriae. The second messenger cyclic di-GMP (cdiGMP), a major regulator of biofilm formation, regulated adherence to the catheter surface in a selected clinical isolate suggesting that the cdiGMP second messenger pathway may be a target for anti-biofilm therapeutic approaches.

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Xiaoda Wang
    • 1
  • Heinrich Lünsdorf
    • 2
  • Ingrid Ehrén
    • 3
  • Annelie Brauner
    • 1
    • 4
  • Ute Römling
    • 1
  1. 1.Department of Microbiology, Tumor and Cell BiologyKarolinska InstitutetStockholmSweden
  2. 2.Department of MicrobiologyHelmholtz Zentrum fur InfektionsforschungBraunschweigGermany
  3. 3.Urology DepartmentKarolinska University HospitalStockholmSweden
  4. 4.Clinical MicrobiologyKarolinska University HospitalStockholmSweden

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