Diversity of Yeasts from Puddles in the Vicinity of Midre Lovénbreen Glacier, Arctic and Bioprospecting for Enzymes and Fatty Acids
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A total of 132 yeast strains were characterised from 4 sediment samples collected from small puddles in the vicinity of Midre Lovénbreen glacier, Arctic. Based on the D1/D2 domain sequence similarity, the isolates could be categorised into 6 groups. The nearest phylogenetic neighbour of groups I to VI were identified as Cryptococcus gastricus, Cryptococcus terricolus, Rhodotorula muscorum, Mrakia psychrophila, Mrakia gelida and Rhodotorula glacialis, respectively. Strains representative of the six groups were psychrophilic and salt tolerant but varied in their ability to produce cold-active extracellular enzymes such as lipase, protease, pectinase, cellulase and amylase. C18:1 (w9C) and C18:2 (w9,12C) were the only two fatty acids common to all the yeasts and branched and (or) unsaturated fatty acids increased in yeasts growing at 8°C compared to 22°C, probably as an adaptation to low temperature. The present study establishes that psychrophilic yeasts are predominant in Arctic and could be used as work horses to produce cold-active enzymes and poly unsaturated fatty acids which have been implicated in low temperature adaptation and also for their use in biotechnology.
KeywordsCellulase Unsaturated Fatty Acid Rhodotorula Yeast Nitrogen Base Tributyrin
We would like to thank the Council of Scientific and Industrial Research, Government of India for financial support to SS. SS would also like to thank NCAOR and Ministry of Earth Sciences, Government of India, for providing the financial and logistic support for the trip to Arctic.
- 1.Abyzov SS (1993) Microorganisms in the Antarctic ice. In: Friedmann EI (ed) Antarctic microbiology. Wiley-Liss, New YorkGoogle Scholar
- 3.Bjornsson H, Gjessing Y, Hamran SE, Hagen JO, Liesto O, Palsson F, Erlingsson B (1996) The thermal regime of sub-polar glaciers mapped by multi-frequency radio-echo sounding. J Glaciol 42:23–32Google Scholar
- 4.Burden DW, Eveleight DE (1990) Yeasts—diverse substrates and products. In: Spencer JFT, Spencer DM (eds) Yeast technology. Springer-Verlag, Berlin, pp 199–227Google Scholar
- 11.Dmitriev VV, Gilichinsky DA, Faizutdinova RN, Shershunov IN, Golubev WI, Duda VI (1997) Occurrence of viable yeasts in 3-million-year-old permafrost in Siberia. Mikrobiologiya 66:655–660Google Scholar
- 14.Jones GEB (1976) Recent advances in aquatic mycology. The Gresham Press, Old Woking, SurreyGoogle Scholar
- 25.Reddy PVV, Rao SSSN, Pratibha MS, Sailaja B, Kavya B, Manorama RR, Singh SM, Srinivas TNR, Shivaji S (2009) Bacterial diversity and bioprospecting for cold-active enzymes from culturable bacteria associated with sediment from a melt water stream of Midtre Lovénbreen glacier, an Arcrtic glacier. Res Microbiol 160:538–546CrossRefGoogle Scholar
- 28.Shivaji S, Prasad GS (2008) Antarctic yeast: biodiversity and potential applications. In: Satyanarayana T, Kunze Gotthard (eds) Yeast biotechnology: diversity & application. Springer, NetherlandsGoogle Scholar
- 29.Shivaji S, Kiran MD, Chintalapati S (2007) In: Gerday C, Glansdorff N (eds) Physiology and biochemistry of extremophiles. ASM Press, Washington, pp 194–207Google Scholar
- 30.Starmer WT, Fell YW, Catranis CM, Aberdeen V, Ma LJ, Zhou S, Rogers SO (2005) Yeasts in the genus Rhodotorula recovered from the Greenland ice sheet. In: Castello JD, Rogers SO (eds) Life in ancient ice. Princeton University Press, Princeton, OxfordGoogle Scholar
- 35.Vishniac HS, Klinger J (1986) Extreme environments. Yeasts in the Antarctic deserts. In: Megusǎr F, Gantar M (eds) Perspectives in microbial ecology. Slovene Society for Microbiology, Ljubljana, SloveniaGoogle Scholar
- 36.Yarrow D (1998) Methods for the isolation, maintenance and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–100Google Scholar