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Current Microbiology

, Volume 60, Issue 4, pp 287–293 | Cite as

Yeast Microflora Isolated From Brazilian Cassava Roots: Taxonomical Classification Based on Molecular Identification

  • Nelson Ferreira
  • Carmela Belloch
  • Amparo Querol
  • Paloma Manzanares
  • Salvador Vallez
  • Alberdan Santos
Article

Abstract

A rapid molecular identification technique was applied on microbial microflora isolated from Brazilian cassava roots given a yeast profile presented in the samples analyzed. A total of 24 strain isolated from cassava were initially grouped and identified in five groups using restriction-fragment length polymorphism (RFLPs) of 5.8S-ITS rDNA region. Sequencing analysis of the domains D1 and D2 of the 26S rRNA gene or 5.8S rRNA-ITS region were used to identify different groups of yeasts. Representative colonies of yeasts of each group were isolated and identified as Debaromyces hansenii, Kodamaea ohmeri, Candida glabrata, C. haemulonii, and Pichia gullhermondii. It is hoped that these results will contribute toward selecting yeast from this microflora capable to degrade cassava starch in the near future.

Keywords

Starch Bark Internal Transcribe Spacer Cassava rDNA Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This work was supported by CSIC/IATA and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Nelson Ferreira
    • 1
  • Carmela Belloch
    • 2
  • Amparo Querol
    • 2
  • Paloma Manzanares
    • 2
  • Salvador Vallez
    • 2
  • Alberdan Santos
    • 1
  1. 1.Universidade Federal do Pará, Laboratório de Investigação Sistemática em Biotecnologia e Química FinaBelémBrazil
  2. 2.Instituto de Agroquimica y Tecnologia de Alimentos, BiotecnologíaValenciaSpain

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