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Current Microbiology

, Volume 60, Issue 4, pp 263–267 | Cite as

Role of the Y-Family DNA Polymerases YqjH and YqjW in Protecting Sporulating Bacillus subtilis Cells from DNA Damage

  • Andrea M. Rivas-Castillo
  • Ronald E. Yasbin
  • E. Robleto
  • Wayne L. Nicholson
  • Mario Pedraza-ReyesEmail author
Article

Abstract

The role played by the Y-family DNA polymerases YqjH and YqjW in protecting sporulating cells of Bacillus subtilis from DNA damage was determined. The absence of either yqjH and/or yqjW not only reduced sporulation efficiency but also sensitized the sporulating cells to hydrogen peroxide, tert-butylhydroperoxide (t-BHP), mitomycin-C (M-C), and UV-C radiation. Moreover, these DNA-damaging agents increased the mutation frequency of wild-type sporulating cells to 4-azaleucine, but the production of mutants was YqjH- and YqjW-dependent. In conclusion, the results presented here indicate that YqjH/YqjW-dependent-translesion synthesis (TLS) operates in sporulating B. subtilis cells and contributes in processing spontaneous and artificially induced genetic damage, which is apparently required for an efficient sporulation process.

Keywords

Subtilis Cell Subtilis Spore Sporulating Cell Sporulation Efficiency Spore Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors wish to thank Patricia Fajardo-Cavazos, Jose Luis Ortiz-Lugo, and Silvia Mellado for invaluable technical assistance. This work was supported by Consejo Nacional de Ciencia y Tecnología from México (CONACyT) grants 43644 and 84482 to M. Pedraza-Reyes and by grants from NASA (NNA06CB58G) and USDA (FLA-MCS-04602) to W.L.N. A.M. Rivas-Castillo was supported by a doctoral scholarship from CONACyT.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Andrea M. Rivas-Castillo
    • 1
  • Ronald E. Yasbin
    • 2
  • E. Robleto
    • 2
  • Wayne L. Nicholson
    • 3
  • Mario Pedraza-Reyes
    • 1
    Email author
  1. 1.Division of Natural and Exact Sciences, Department of BiologyUniversity of GuanajuatoGuanajuatoMéxico
  2. 2.School of Life SciencesUniversity of Nevada-Las VegasLas VegasUSA
  3. 3.Department of Microbiology and Cell ScienceUniversity of Florida, Space Life Sciences LaboratoryKennedy Space CenterUSA

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