Role of the Y-Family DNA Polymerases YqjH and YqjW in Protecting Sporulating Bacillus subtilis Cells from DNA Damage
- 313 Downloads
The role played by the Y-family DNA polymerases YqjH and YqjW in protecting sporulating cells of Bacillus subtilis from DNA damage was determined. The absence of either yqjH and/or yqjW not only reduced sporulation efficiency but also sensitized the sporulating cells to hydrogen peroxide, tert-butylhydroperoxide (t-BHP), mitomycin-C (M-C), and UV-C radiation. Moreover, these DNA-damaging agents increased the mutation frequency of wild-type sporulating cells to 4-azaleucine, but the production of mutants was YqjH- and YqjW-dependent. In conclusion, the results presented here indicate that YqjH/YqjW-dependent-translesion synthesis (TLS) operates in sporulating B. subtilis cells and contributes in processing spontaneous and artificially induced genetic damage, which is apparently required for an efficient sporulation process.
KeywordsSubtilis Cell Subtilis Spore Sporulating Cell Sporulation Efficiency Spore Resistance
The authors wish to thank Patricia Fajardo-Cavazos, Jose Luis Ortiz-Lugo, and Silvia Mellado for invaluable technical assistance. This work was supported by Consejo Nacional de Ciencia y Tecnología from México (CONACyT) grants 43644 and 84482 to M. Pedraza-Reyes and by grants from NASA (NNA06CB58G) and USDA (FLA-MCS-04602) to W.L.N. A.M. Rivas-Castillo was supported by a doctoral scholarship from CONACyT.
- 5.Miller J (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USAGoogle Scholar