Tocopherol, Carotene, Phenolic Contents and Antibacterial Properties of Rose Essential Oil, Hydrosol and Absolute
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The antioxidant and antibacterial activities, and total phenolic contents of Rosa damascena Mill. flower extracts (absolute, essential oil and hydrosol) were investigated. The chemical compositions of these extracts were analysed by GC-MS. Phenylethyl alcohol (78.38%) was found to be the main constituent of rose absolute, while citrenellol and geraniol were the major compounds (>55%) of rose essential oil and hydrosol. Tocopherol and carotene levels were determined by high performance liquid chromatography (HPLC) analysis. The levels of beta carotene (422.3±35.6 ppm), alpha tocopherol (2397.1±72.5 ppm) and gamma tocopherol (343.1±28.4 ppm) of rose absolute were found to be higher than that of essential oil and hydrosol. Their total phenolic contents were also evaluated. The total phenolic content of the tested extracts varied from 5.2 to 2134.3 GAE/mg L−1. Rose absolute and essential oil contained high levels of phenolics and demonstrated strong antibacterial activity against Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 6538), Chromobacterium violaceum (ATCC 12472) and Erwinia carotovora (ATCC 39048) strains.
KeywordsTocopherol High Performance Liquid Chromatography Total Phenolic Content Carotene Geraniol
We thank to the Sebat Ltd., Isparta for kindly providing rose essential oil, hydrosol and rose absolute.
- 1.Kaul VK, Singh V, Singh B (2000) Damask rose and marigold: prospective industrial crops. J Med Aromat Plant Sci 22:313–318Google Scholar
- 7.Kumar N, Bhandari P, Singh B, Bari SS (2008) Antioxidant activity and ultra-performance LC-electrospray ionization quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, R. bourboniana and R. brunonii. Food Chem Toxicol 47:361–367PubMedGoogle Scholar
- 13.Gochev V, Wlcek K, Buchbauer G, Stoyanova A, Dobreva A, Schmidt E, Jirovetz L (2008) Comparative evaluation of antimicrobial activity and composition of rose oils from various geographic origins, in particular Bulgarian rose oil. Nat Prod Commun 3:1063–1068Google Scholar
- 16.Singleton VL, Rossi JR (1965) Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid. Am J Enology and Vitic 16:144–158Google Scholar
- 17.Jirovetz L, Buchbauer G, Shahabi M (2002) Comparative investigations of essential oils and their SPME headspace volatiles of Rosa damascena from Bulgaria and Rosa centifolia from Morocco using GC-FID, GC–MS and olfactometry. J Essent Oil-Bear Plants 5:111–121Google Scholar
- 19.Loghmani-Khouzani H, Sabzi-Fini O, Safari J (2007) Essential oil composition of Rosa damascena Mill cultivated in central Iran. Sci Iran 14:316–319Google Scholar
- 22.Jassbi AR, Zamanizadehnajarib S, Tahara S (2003) Polyphenolic antioxidant constituents of Rosa persica. J Chem Soc Pak 25:323–327Google Scholar
- 26.Fabre CE, Blanc PJ, Goma G (1998) 2-Phenylethyl alcohol: an aroma profile. Perfume Flavor 23:43–45Google Scholar