Current Microbiology

, Volume 59, Issue 5, pp 489–496 | Cite as

Genetic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Rhizospheric Soil of Wheat Under Saline Condition

  • Sudhir K. Upadhyay
  • Devendra P. Singh
  • Ratul Saikia
Article

Abstract

In this study, a total of 130 rhizobacteria was isolated from a saline infested zone of wheat rhizosphere, and screened for plant growth promoting (PGP) traits at higher salt (NaCl) concentrations (2, 4, 6, and 8%). The results revealed that 24 rhizobacterial isolates were tolerant at 8% NaCl. Although all the 24 salt tolerable isolates produced indole-3-acetic acid (IAA), while 10 isolates solubilized phosphorus, eight produced siderophore, and six produced gibberellin. However, only three isolates showed the production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase. Diversity was analyzed through 16S rDNA-RFLP, and of these isolates with three tetra cutter restriction enzymes (HaeIII, AluI, and MspI), the representative cluster groups were identified by 16S rDNA sequencing. Bacillus and Bacillus-derived genera were dominant which showed PGP attributes at 8% NaCl concentration. Out of 24 isolates, nitrogen fixing ability (nif H gene) was detected in the two isolates, SU18 (Arthrobacter sp.) and SU48.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Sudhir K. Upadhyay
    • 1
  • Devendra P. Singh
    • 1
  • Ratul Saikia
    • 2
  1. 1.Department of Environmental SciencesB.B. Ambedkar UniversityLucknowIndia
  2. 2.Biotechnology DivisionNorth-East Institute of Science and Technology (CSIR)JorhatIndia

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