Current Microbiology

, Volume 59, Issue 4, pp 469–474 | Cite as

Interference of Cranberry Constituents in Cell–Cell Signaling System of Vibrio harveyi

  • Mark Feldman
  • Ervin I. Weiss
  • Itzhak Ofek
  • Doron Steinberg
Article

Abstract

Cranberry juice has long been recognized in folk medicine as a therapeutic agent, mainly in urinary track infections. It acts as an antibiofilm agent against various pathogens. Quorum sensing is process where bacteria communicate with each other via signal molecules known as autoinducers. This process is strongly involved in various bacterial pathological and physiological pathways. Various strains of Vibrio harveyi bacteria were incubated with different concentrations of nondialyzable material of cranberry (NDM) with or without addition of exogenous autoinducer. Bioluminescence regulated by the autoinducers was measured in GENios reader. Effect of NDM alone or NDM supplemented with autoinducer on quorum sensing was determined as change in bioluminescence in each treated sample compared to appropriate control in every strain. Using model of V. harveyi, we found an inhibitory effect of cranberry constituents on bacterial signaling system. This effect was reversible, since exogenous autoinducer was able to recover bioluminescence which was decreased by NDM. We hypothesized that cranberry NDM interacts with V. harveyi quorum sensing by competition with autoinducer for binding to autoinducer sensor.

References

  1. 1.
    Adonizio A, Kong KF, Mathee K (2008) Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrob Agents Chemother 52:198–203PubMedCrossRefGoogle Scholar
  2. 2.
    Aharoni R, Bronstheyn M, Jabbour A, Zaks B, Srebnik M, Steinberg D (2008) Oxazaborolidine derivatives inducing autoinducer-2 signal transduction in Vibrio harveyi. Bioorg Med Chem 16:1596–1604PubMedCrossRefGoogle Scholar
  3. 3.
    Bassler BL, Wright M, Showalter RE, Silverman MR (1993) Intercellular signalling in Vibrio harveyi: sequence and function of genes regulating expression of luminescence. Mol Microbiol 9:773–786PubMedCrossRefGoogle Scholar
  4. 4.
    Bassler BL, Wright M, Silverman MR (1994) Sequence and function of LuxO, a negative regulator of luminescence in Vibrio harveyi. Mol Microbiol 12:403–412PubMedCrossRefGoogle Scholar
  5. 5.
    Bassler BL, Greenberg EP, Stevens AM (1997) Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. J Bacteriol 179:4043–4045PubMedGoogle Scholar
  6. 6.
    Bodet C, Piché M, Chandad F, Grenier D (2006) Inhibition of periodontopathogenderived proteolytic enzymes by a high-molecular-weight fraction isolated from cranberry. J Antimicrob Chemother 57:685–690PubMedCrossRefGoogle Scholar
  7. 7.
    Bosgelmez-Tinaz G, Ulusoy S, Ugur A, Ceylan O (2007) Inhibition of quorumsensing-regulated behaviors by Scorzonera sandrasica. Curr Microbiol 55:114–118PubMedCrossRefGoogle Scholar
  8. 8.
    Burger O, Ofek I, Tabak M, Weiss EI, Sharon N, Neeman I (2000) A high molecular mass constituent of cranberry juice inhibits helicobacter pylori adhesion to human gastric mucus. FEMS Immunol Med Microbiol 29:295–301PubMedCrossRefGoogle Scholar
  9. 9.
    Choo JH, Rukayadi Y, Hwang JK (2006) Inhibition of bacterial quorum sensing by vanilla extract. Lett Appl Microbiol 42:637–641PubMedGoogle Scholar
  10. 10.
    Duarte S, Gregoire S, Singh AP, Vorsa N, Schaich K, Bowen WH, Koo H (2006) Inhibitory effects of cranberry polyphenols on formation and acidogenicity of Streptococcus mutans biofilms. FEMS Microbiol Lett 257:50–56PubMedCrossRefGoogle Scholar
  11. 11.
    Feldman M, Weiss EI, Shemesh M, Ofek I, Bachrach G, Rozen R, Steinberg D (2009) Cranberry constituents affect fructosyltransferase expression in Streptococcus mutans. Alternat Ther Health Med 15:32–38Google Scholar
  12. 12.
    Fux CA, Costerton JW, Stewart PS, Stoodley P (2005) Survival strategies of infectious biofilms. Trends Microbiol 13:34–40PubMedCrossRefGoogle Scholar
  13. 13.
    Higgins DA, Pomianek ME, Kraml CM, Taylor RK, Semmelhack MF, Bassler BL (2007) The major Vibrio cholerae autoinducer and its role in virulence factor production. Nature 450:883–886PubMedCrossRefGoogle Scholar
  14. 14.
    Hochman N, Houri-Haddad Y, Koblinski J, Wahl L, Roniger M, Bar-Sinai A, Weiss EI, Hochman J (2008) Cranberry juice constituents impair lymphoma growth and augment the generation of antilymphoma antibodies in syngeneic mice. Nutr Cancer 60:511–517PubMedCrossRefGoogle Scholar
  15. 15.
    Koo H, Nino de Guzman P, Schobel BD, Vacca Smith AV, Bowen WH (2006) Influence of cranberry juice on glucan-mediated processes involved in Streptococcus mutans biofilm development. Caries Res 40:20–27PubMedCrossRefGoogle Scholar
  16. 16.
    Labrecque J, Bodet C, Chandad F, Grenier D (2006) Effects of a high-molecular weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis. J Antimicrob Chemother 58:439–443PubMedCrossRefGoogle Scholar
  17. 17.
    Leitão DP, Polizello AC, Ito IY, Spadaro AC (2005) Antibacterial screening of anthocyanic and proanthocyanic fractions from cranberry juice. J Med Food 8:36–40PubMedCrossRefGoogle Scholar
  18. 18.
    Liu Y, Black MA, Caron L, Camesano TA (2006) Role of cranberry juice on molecular-scale surface characteristics and adhesion behavior of Escherichia coli. Biotechnol Bioeng 93:297–305PubMedCrossRefGoogle Scholar
  19. 19.
    Steinberg D, Feldman M, Ofek I, Weiss EI (2004) Effect of a high-molecularweight component of cranberry on constituents of dental biofilm. J Antimicrob Chemother 54:86–89PubMedCrossRefGoogle Scholar
  20. 20.
    Rasmussen TB, Bjarnsholt T, Skindersoe ME, Hentzer M, Kristoffersen P, Köte M, Nielsen J, Eberl L, Givskov M (2005) Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. J Bacteriol 187:1799–1814PubMedCrossRefGoogle Scholar
  21. 21.
    Steinberg D, Feldman M, Ofek I, Weiss EI (2005) Cranberry high molecular weight constituents promote Streptococcus sobrinus desorption from artificial biofilm. Int J Antimicrob Agent 25:247–251CrossRefGoogle Scholar
  22. 22.
    Weiss EI, Kozlovsky A, Steinberg D, Lev-Dor R, Bar Ness Greenstein R, Feldman M, Sharon N, Ofek I (2004) A high molecular mass cranberry constituent reduces mutans streptococci level in saliva and inhibits in vitro adhesion to hydroxyapatite. FEMS Microbiol Lett 232:89–92PubMedCrossRefGoogle Scholar
  23. 23.
    Weiss EI, Houri-Haddad Y, Greenbaum E, Hochman N, Ofek I, Zakay-Rones Z (2005) Cranberry juice constituents affect influenza virus adhesion and infectivity. Antiviral Res 66:9–12PubMedCrossRefGoogle Scholar
  24. 24.
    Yamanaka A, Kouchi T, Kasai K, Kato T, Ishihara K, Okuda K (2007) Inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of Porphyromonas gingivalis. J Periodontal Res 42:589–592PubMedCrossRefGoogle Scholar
  25. 25.
    Zhang L, Ma J, Pan K, Go VL, Chen J, You WC (2005) Efficacy of cranberry juice on Helicobacter pylori infection: a double-blind, randomized placebo-controlled trial. Helicobacter 10:139–145PubMedCrossRefGoogle Scholar
  26. 26.
    Zhu H, Sun SJ (2008) Inhibition of bacterial quorum sensing-regulated behaviors by Tremella fuciformis extract. Curr Microbiol 57:418–422PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Mark Feldman
    • 1
    • 2
  • Ervin I. Weiss
    • 2
  • Itzhak Ofek
    • 3
  • Doron Steinberg
    • 1
  1. 1.Institute of Dental Sciences, Faculty of Dental MedicineHebrew University-HadassahJerusalemIsrael
  2. 2.Department of Prosthodontics, Faculty of Dental MedicineHebrew University-HadassahJerusalemIsrael
  3. 3.Department of Human Microbiology, Sackler Faculty of MedicineTel Aviv UniversityTel AvivIsrael

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