Current Microbiology

, Volume 59, Issue 4, pp 432–438 | Cite as

Mountain Aspect Influences the Genetic Clustering of Psychrotolerant Phosphate Solubilizing Pseudomonads in the Uttarakhand Himalayas

  • Govindan SelvakumarEmail author
  • Piyush Joshi
  • Pankaj K. Mishra
  • Jaideep K. Bisht
  • Hari S. Gupta


Fourteen cold tolerant phosphate solubilizing bacteria isolated from high altitude representative locations of the two major mountain aspects of the Uttarakhand Himalayas (cooler north and warmer south facing slopes) were selected for this study. The tricalcium phosphate (TCP) solubilizing abilities of the isolates were estimated at three different incubation temperatures viz., 4, 15, and 30°C under in vitro conditions. Irrespective of their geographical origin, all the isolates recorded maximum P release values at 30°C. At 4°C, the isolates from the north facing slope were found to release significantly higher levels of P, as compared to the isolates from the south facing slopes. Alternatively at 15°C, the isolates from the south facing slope were found to release significantly higher levels of P. Initial confirmation of their genus level identity as Pseudomonads was arrived by amplification of a 990 bp fragment of the 16S rRNA gene using genus specific primers. Further putative species level identification was arrived by sequencing of the 16S rRNA gene. The diversity among the isolates was determined by rep-PCR using the primers BOX, ERIC, and ERIC2. A composite dendrogram constructed using the rep-PCR profiles revealed that the isolates from the north and south mountain aspects formed separate major clusters. The extent of diversity was greater among the isolates from the south mountain aspect. This study reveals the potential of rep-PCR in determining the genetic diversity among Pseudomonads selected for a single functional trait, but varying in their geographical origin.


Phosphate Solubilization Minor Cluster Plant Growth Promotional Trait Enterobacterial Repetitive Intergenic Consensus Repetitive Extragenic Palindromic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Part of this research work was supported by ICAR, under the project “Application of Microorganisms in Agriculture and Allied Sectors”. Mr. Sanjay Kumar is acknowledged for technical assistance.


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Govindan Selvakumar
    • 1
    Email author
  • Piyush Joshi
    • 1
  • Pankaj K. Mishra
    • 1
  • Jaideep K. Bisht
    • 1
  • Hari S. Gupta
    • 1
  1. 1.Vivekananda Institute of Hill AgricultureIndian Council of Agricultural ResearchAlmoraIndia

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