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Current Microbiology

, Volume 59, Issue 3, pp 256–260 | Cite as

Degradation of Trichloroethylene by Bacillus sp.: Isolation Strategy, Strain Characteristics, and Cell Immobilization

  • Kaushik Dey
  • Pranab RoyEmail author
Article

Abstract

A novel isolate of a bacterium, capable of degrading trichloroethylene (TCE) and growing on this as the sole carbon source is reported. The test strain was isolated by an enrichment technique with trichloroethylene as the substrate. The isolated strain belongs to the genus Bacillus. The practical utility of cleaning up oil spillage by bioremediation could be extended to this bacterium to degrade the environmental pollutant, which is used in metal degreasing in industries. Cells of the novel bacterium immobilized on calcium alginate were found to have better trichloroethylene degrading activity than the ones which were immobilized on agar-agar or free cells.

Keywords

Sole Carbon Source Trichloroethylene Calcium Alginate Methanotrophs Microbial Type Culture Collection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors gratefully acknowledge the help from Dr. S. S. Mishra, Mr. Utpal Bhowmik, Principle Scientists, Central Inland Fisheries Research Institute, Barrackpore, West Bengal and Mr. R. N. Mazumdar, Chairman, Rahul Foundation during the entire course of investigation.

References

  1. 1.
    DeBaeres S, Mayer S, Dirinck I, Lambert W, Piette M, VanPeteghem C, De Leenheer A (1997) Tissue distribution of trichloroethylene and its metabolites in a forensic science. J Anal Toxicol 21:223–227Google Scholar
  2. 2.
    Ensley BD (1991) Biochemical diversity of trichloroethylene metabolism. Annu Rev Microbiol 45:283–299PubMedCrossRefGoogle Scholar
  3. 3.
    Glick BR, Pasternak JJ (2007) Bioremediation and biomass utilization. 3rd Indian Reprint, ASM Press, Washington DC, 387 ppGoogle Scholar
  4. 4.
    He J, Ritalahti MK, Aiello RM, Löffler EF (2003) Complete detoxification of vinyl chloride by an anaerobic enrichment culture and identification of the reductively dechlorinating population as a Dehalococcoides Species. Appl Environ Microbiol 69:996–1003PubMedCrossRefGoogle Scholar
  5. 5.
    Jahng D, Wood KT (1994) Trichloroethylene and chloroform degradation by a recombinant Pseudomonad expressing soluble methane monooxygenase from Methylosinus trichosporium OB3b. Appl Environ Microbiol 60:2473–2484PubMedGoogle Scholar
  6. 6.
    Moss MS, Rylance HJ (1966) The Fujiwara reaction: some observation on the mechanism. Nature 210:945–946CrossRefGoogle Scholar
  7. 7.
    Mu YD, Scow MK (1994) Effect of trichloroethylene (TCE) and toluene concentrations on TCE and toluene biodegradation and the population density of TCE and toluene degrader in soil. Appl Environ Microbiol 60:2661–2665PubMedGoogle Scholar
  8. 8.
    Oldenhuis R, Oedzes YJ, Warrde JJ, Janssen BD (1991) Kinetics of chlorinated hydrocarbon degradation by Methylosinus trichosporium OB3b and toxicity of trichloroethylane. Appl Envinon Microbiol 57:7–14Google Scholar
  9. 9.
    Prescott LM, Harley JP, Klein DA (2005) Clinical Microbiology, 6th edn. McGraw Hill Higher education, New York, p 812Google Scholar
  10. 10.
    Wackett PL, Gibson TD (1988) Degradation of trichloroethylene by toluene dioxygenase in whole-cell studies with Pseudomonas putida Fl. Appl Environ Microbiol 54:1703–1708PubMedGoogle Scholar
  11. 11.
    Yee CD, Maynard AJ, Wood KT (1998) Rhizoremediation of trichloroethylene by a recombinant, root-colonizing Pseudomonas fluorescens strain expressing toluene ortho-monooxygenase constitutively. Appl Environ Microbiol 64:112–118PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Durgapur College of Commerce & ScienceBurdwanIndia
  2. 2.Department of BiotechnologyUniversity of BurdwanBurdwanIndia

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