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Current Microbiology

, 58:404 | Cite as

Identification of Cellulase Genes from the Metagenomes of Compost Soils and Functional Characterization of One Novel Endoglucanase

  • Hao Pang
  • Peng Zhang
  • Cheng-Jie Duan
  • Xin-Chun Mo
  • Ji-Liang Tang
  • Jia-Xun FengEmail author
Article

Abstract

Metagenomics, a new research field developed over the past decade, aims to identify potential enzymes from nonculturable microbes. In this study, genes encoding three glycoside hydrolase family (GHF) 9 endoglucanases and one GHF 5 endoglucanase were cloned and identified from the metagenome of the compost soils. The shared identities between the predicted amino acid sequences of these genes and their closest homologues in the database were less than 70%. One GHF 9 endoglucanase, Umcel9B, was further characterized. The recombinant protein, Umcel9B, showed activity against carboxymethyl cellulose, indicating that Umcel9B is an endoactive enzyme. Enzymatic activity occurs optimally at a pH of 7.0 and a temperature of 25°C.

Keywords

Cellulase Bacterial Artificial Chromosome Glycoside Hydrolase Family Metagenomic Library Cellulase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This work was supported by Grants for New Century Excellent Talents in Universities of China (NCET-05-0752), the National Natural Science Foundation of China (30560003) and Hi-tech Research and Development Program of China (863 Program 2007AA021307).

Supplementary material

284_2008_9346_MOESM1_ESM.rtf (311 kb)
Supplementary material 1 (RTF 311 kb)

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hao Pang
    • 1
    • 2
  • Peng Zhang
    • 1
    • 2
  • Cheng-Jie Duan
    • 1
    • 2
  • Xin-Chun Mo
    • 1
    • 2
  • Ji-Liang Tang
    • 1
    • 2
  • Jia-Xun Feng
    • 1
    • 2
    Email author
  1. 1.Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, The Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering, College of Life Science and TechnologyGuangxi UniversityNanning, GuangxiPeople’s Republic of China
  2. 2.Guangxi Key Laboratory of BioIndustry TechnologyNanning, Guangxi People’s Republic of China

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