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Current Microbiology

, Volume 58, Issue 4, pp 371–377 | Cite as

Plant Growth-Promoting and Rhizosphere-Competent Acinetobacter rhizosphaerae Strain BIHB 723 from the Cold Deserts of the Himalayas

  • Arvind GulatiEmail author
  • Pratibha Vyas
  • Praveen Rahi
  • Ramesh Chand Kasana
Article

Abstract

A phosphate-solubilizing bacterial strain BIHB 723 isolated from the rhizosphere of Hippophae rhamnoides was identified as Acinetobacter rhizosphaerae on the basis of phenotypic characteristics, carbon source utilization pattern, fatty acid methyl esters analysis, and 16S rRNA gene sequence. The strain exhibited the plant growth-promoting attributes of inorganic and organic phosphate solubilization, auxin production, 1-aminocyclopropane-1-carboxylate deaminase activity, ammonia generation, and siderophore production. A significant increase in the growth of pea, chickpea, maize, and barley was recorded for inoculations under controlled conditions. Field testing with the pea also showed a significant increment in plant growth and yield. The rifampicin mutant of the bacterial strain effectively colonized the pea rhizosphere without adversely affecting the resident microbial populations.

Keywords

Acinetobacter Total Viable Count Trypticase Soya Agar Uninoculated Plant Acinetobacter Calcoaceticus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors acknowledge the Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh, India for FAME analysis. They also acknowledge the Director of the Institute of Himalayan Bioresource Technology for providing the necessary facilities. The Council of Scientific and Industrial Research, Government of India, also is acknowledged for financial support under the CSIR Network Project “Exploitation of India’s Rich Microbial Wealth” (NWP 006).

References

  1. 1.
    Ahmad F, Ahmad I, Khan MS (2008) Screening of free-living rhizospheric bacteria for their multiple plant growth-promoting activities. Microbiol Res 163:173–181PubMedCrossRefGoogle Scholar
  2. 2.
    Albert F, Anderson AJ (1987) The effect of Pseudomonas putida colonization on root surface peroxidases. Plant Physiol 85:535–541CrossRefGoogle Scholar
  3. 3.
    Bakker AW, Schippers B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth reduction. Soil Biol Biochem 19:452–458Google Scholar
  4. 4.
    Cappuccino JC, Sherman N (1992) Microbiology: a laboratory manual. Benjamin/Cummings Publishing Co., New York, pp 125–179Google Scholar
  5. 5.
    Cattelan AJ, Hartel PG, Fuhrmann JJ (1999) Screening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Sci Soc Am J 63:1670–1680Google Scholar
  6. 6.
    Dey R, Pal KK, Bhatt DM, Chauhan SM (2004) Growth promotion and yield enhancement of peanut (Arachis hypogaea L.) by application of plant growth-promoting rhizobacteria. Microbiol Res 159:371–394PubMedCrossRefGoogle Scholar
  7. 7.
    Duponois R, Kisa M, Plenchette C (2006) Phosphate-solubilizing potential of the nematophagous fungus Arthrobotrys oligospora. J Plant Nutr Soil Sci 169:280–282CrossRefGoogle Scholar
  8. 8.
    Glandorf DCM, Sluis IV, Anderson AJ et al (1994) Agglutinization, adherence, and root colonization by fluorescent pseudomonads. Appl Environ Microbiol 60:1726–1733PubMedGoogle Scholar
  9. 9.
    Glick BR (1995) The enhancement of plant growth by free-living bacteria. Can J Microbiol 41:109–117CrossRefGoogle Scholar
  10. 10.
    Gulati A, Rahi P, Vyas P (2008) Characterization of phosphate-solubilizing fluorescent pseudomonads from rhizosphere of seabuckthorn growing in cold deserts of Himalayas. Curr Microbiol 56:73–79PubMedCrossRefGoogle Scholar
  11. 11.
    Hoagland DR, Arnon DI (1938) The water-culture methods for growing plants without soil. Circ Calif Agric Expt Stn Ext Serv, Berkeley, California, p 347Google Scholar
  12. 12.
    Indiragandhi P, Anandham R, Madhaiyan M, Sa TM (2008) Characterization of plant growth-promoting traits of bacteria isolated from larval guts of Diamondback moth Plutella xylostella (Lepidoptera: Plutellidae). Curr Microbiol 56:327–333PubMedCrossRefGoogle Scholar
  13. 13.
    Jacobson BC, Pasternak JJ, Glick BR (1994) Partial purification and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the plant growth-promoting rhizobacterium Pseudomonas putida GR 12–2. Can J Microbiol 40:1019–1025CrossRefGoogle Scholar
  14. 14.
    Johri JK, Surange S, Nautiyal CS (1999) Occurrence of salt-, pH-, and temperature-tolerant phosphate-solubilizing bacteria in alkaline soils. Curr Microbiol 39:89–93PubMedCrossRefGoogle Scholar
  15. 15.
    Kravchenko LV, Azarova TS, Makarova NM, Tikhonovich IA (2004) The effect of tryptophan present in plant root exudates on the phytostimulating activity of rhizobacteria. Microbiology 73:156–158CrossRefGoogle Scholar
  16. 16.
    Krieg NR, Holt JG (1984) Bergey’s manual of systematic bacteriology, vol. 1. Williams and Willkins, Baltimore, p 964Google Scholar
  17. 17.
    Kuklinsky-Sobrat J, Araujo WL, Mendes R et al (2004) Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion. Environ Microbiol 6:1244–1251CrossRefGoogle Scholar
  18. 18.
    Loper JE, Schroth MN (1986) Influence of bacterial sources on indole-3-acetic acid on root elongation of sugarbeet. Phytopathology 76:386–389CrossRefGoogle Scholar
  19. 19.
    Lottmann J, Heuer H, de Vries J et al (2000) Establishment of introduced antagonistic bacteria in the rhizosphere of transgenic potatoes and their effect on the bacterial community. FEMS Microbiol Ecol 33:41–49PubMedCrossRefGoogle Scholar
  20. 20.
    Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate-solubilizing microorganisms. FEMS Microbiol Lett 170:265–270PubMedCrossRefGoogle Scholar
  21. 21.
    Patten CL, Glick BR (1996) Bacterial biosynthesis of indole-3-acetic acid. Can J Microbiol 42:207–220PubMedGoogle Scholar
  22. 22.
    Payne SM (1994) Detection, isolation, and characterization of siderophores. Methods Enzymol 235:329–344PubMedCrossRefGoogle Scholar
  23. 23.
    Rodriguez R, Fraga R, Gonzalez T, Bashan Y (2006) Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria. Plant Soil 287:15–21CrossRefGoogle Scholar
  24. 24.
    Rodriguez H, Vessely S, Shah S, Glick BR (2008) Effect of a nickel-tolerant ACC deaminase-producing Pseudomonas strain on growth of nontransformed and transgenic canola plants. Curr Microbiol 57:170–174PubMedCrossRefGoogle Scholar
  25. 25.
    Richardson AE, Hadobas PA (1997) Soil isolates of Pseudomonas spp. that utilize inositol phosphates. Can J Microbiol 43:509–516PubMedGoogle Scholar
  26. 26.
    Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56PubMedCrossRefGoogle Scholar
  27. 27.
    Singh RP, Gupta MK (1990) Soil and vegetation study of Lahaul and Spiti cold desert of Western Himalayas. Indian Forester 116:785–790Google Scholar
  28. 28.
    Son HJ, Park GT, Cha MS, Heo MS (2006) Solubilization of insoluble inorganic phosphates by a novel salt and pH-tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Bioresour Technol 97:204–210PubMedCrossRefGoogle Scholar
  29. 29.
    Tsavkelova EA, Cherdyntseva TA, Botina SG, Netrusov AI (2007) Bacteria associated with orchid roots and microbial production of auxin. Microbiol Res 162:69–76PubMedCrossRefGoogle Scholar
  30. 30.
    Vargas FRD, O’Hara GW (2006) Isolation and characterization of rhizosphere bacteria with potential for biological control of weeds in vineyards. J Appl Microbiol 100:946–954CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Arvind Gulati
    • 1
    Email author
  • Pratibha Vyas
    • 1
  • Praveen Rahi
    • 1
  • Ramesh Chand Kasana
    • 1
  1. 1.Plant Pathology and Microbiology Laboratory, Hill Area Tea SciencesInstitute of Himalayan Bioresource TechnologyPalampurIndia

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