Current Microbiology

, Volume 58, Issue 4, pp 371–377 | Cite as

Plant Growth-Promoting and Rhizosphere-Competent Acinetobacter rhizosphaerae Strain BIHB 723 from the Cold Deserts of the Himalayas

  • Arvind GulatiEmail author
  • Pratibha Vyas
  • Praveen Rahi
  • Ramesh Chand Kasana


A phosphate-solubilizing bacterial strain BIHB 723 isolated from the rhizosphere of Hippophae rhamnoides was identified as Acinetobacter rhizosphaerae on the basis of phenotypic characteristics, carbon source utilization pattern, fatty acid methyl esters analysis, and 16S rRNA gene sequence. The strain exhibited the plant growth-promoting attributes of inorganic and organic phosphate solubilization, auxin production, 1-aminocyclopropane-1-carboxylate deaminase activity, ammonia generation, and siderophore production. A significant increase in the growth of pea, chickpea, maize, and barley was recorded for inoculations under controlled conditions. Field testing with the pea also showed a significant increment in plant growth and yield. The rifampicin mutant of the bacterial strain effectively colonized the pea rhizosphere without adversely affecting the resident microbial populations.


Acinetobacter Total Viable Count Trypticase Soya Agar Uninoculated Plant Acinetobacter Calcoaceticus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors acknowledge the Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh, India for FAME analysis. They also acknowledge the Director of the Institute of Himalayan Bioresource Technology for providing the necessary facilities. The Council of Scientific and Industrial Research, Government of India, also is acknowledged for financial support under the CSIR Network Project “Exploitation of India’s Rich Microbial Wealth” (NWP 006).


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Arvind Gulati
    • 1
    Email author
  • Pratibha Vyas
    • 1
  • Praveen Rahi
    • 1
  • Ramesh Chand Kasana
    • 1
  1. 1.Plant Pathology and Microbiology Laboratory, Hill Area Tea SciencesInstitute of Himalayan Bioresource TechnologyPalampurIndia

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