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Current Microbiology

, Volume 58, Issue 2, pp 177–181 | Cite as

Identification of Iron Transporters Expressed in the Magnetotactic Bacterium Magnetospirillum magnetotacticum

  • Azuma Taoka
  • Chihiro Umeyama
  • Yoshihiro Fukumori
Article

Abstract

The magnetotactic bacterium Magnetospirillum magnetotacticum MS-1 mineralizes the magnetite (Fe3O4) crystal and organizes a highly ordered intracellular structure, called the magnetosome. However, the iron transport system, which supports the biogenesis of magnetite, is not fully understood. In this study, we first identified the expressions of both the ferric and the ferrous iron transporter proteins in M. magnetotacticum. The cellular protein compositions of ferric and ferrous iron-rich cultures were examined using two-dimensional electrophoresis. According to the gel patterns, two outer-membrane ferric-siderophore receptor homologues were identified as proteins strongly induced in the ferrous iron-rich condition. Also, we identified for the first time that the ferrous iron transport protein, FeoB, is expressed in the M. magnetotacticum cytoplasmic membrane using immunoblotting.

Keywords

Ferrous Iron Sodium Ascorbate Magnetotactic Bacterium Magnetospirillum Iron Uptake System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by a Human Frontier Science Program Research Grant (RGP0035/2004-C104) to Y.F. and a Grant-in-Aid for Scientific Research on Priority Areas (16087205) to Y.F. from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

References

  1. 1.
    Altschul SF, Gish W, Miller W et al (1990) Basic local alignment search tool. J Mol Biol 215:403–410PubMedGoogle Scholar
  2. 2.
    Andrews SC, Robinson AK, Rodríguez-Quiñones F (2003) Bacterial iron homeostasis. FEMS Microbiol Rev 27:215–237PubMedCrossRefGoogle Scholar
  3. 3.
    Bazylinski DA, Frankel RB (2004) Magnetosome formation in prokaryotes. Nat Rev Microbiol 2:217–230PubMedCrossRefGoogle Scholar
  4. 4.
    Blakemore RP, Maratea D, Wolfe RS (1979) Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium. J Bacteriol 140:720–729PubMedGoogle Scholar
  5. 5.
    Calugay RJ, Miyashita H, Okamura Y et al (2003) Siderophore production by the magnetic bacterium Magnetospirillum magneticum AMB-1. FEMS Microbiol Lett 218:371–375PubMedCrossRefGoogle Scholar
  6. 6.
    Calugay RJ, Takeyama H, Mukoyama D et al (2006) Catechol siderophore excretion by magnetotactic bacterium Magnetospirillum magneticum AMB-1. J Biosci Bioeng 101:445–447PubMedCrossRefGoogle Scholar
  7. 7.
    Cartron ML, Maddocks S, Gillingham P et al (2006) Feo-transport of ferrous iron into bacteria. Biometals 19:143–157PubMedCrossRefGoogle Scholar
  8. 8.
    Crosa JH, Mey AR, Payne SM (2004) Iron transport in bacteria. ASM Press, Washington, DCGoogle Scholar
  9. 9.
    Labarga A, Valentin F, Anderson M et al (2007) Web services at the European Bioinformatics Institute. Nucleic Acids Research Web Services Issue 2007Google Scholar
  10. 10.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685PubMedCrossRefGoogle Scholar
  11. 11.
    Marlovits TC, Haase W, Herrmann C et al (2002) The membrane protein FeoB contains an intramolecular G protein essential for Fe(II) uptake in bacteria. Proc Natl Acad Sci USA 99:16243–16248PubMedCrossRefGoogle Scholar
  12. 12.
    Mizushima S, Yamada H (1975) Isolation and characterization of two outer membrane preparations from Escherichia coli. Biochim Biophys Acta 375:44–53PubMedCrossRefGoogle Scholar
  13. 13.
    Nakai K, Kanehisa M (1991) Expert system for predicting protein localization sites in gram-negative bacteria. Proteins 11:95–110PubMedCrossRefGoogle Scholar
  14. 14.
    O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021PubMedGoogle Scholar
  15. 15.
    Paoletti LC, Blakemore RP (1989) Hydroxamate production by Aquaspirillum magnetotacticum. J Bacteriol 167:73–76Google Scholar
  16. 16.
    Rong C, Huang Y, Zhang W et al (2008) Ferrous iron transport protein B gene (feoB1) plays an accessory role in magnetosome formation in Magnetospirillum gryphiswaldense strain MSR–1. Res Microbiol 159(7/8):530–536PubMedCrossRefGoogle Scholar
  17. 17.
    Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
  18. 18.
    Schüler D, Baeuerlein E (1996) Iron-limited growth and kinetics of iron uptake in Magnetospirillum gryphiswaldense. Arch Microbiol 166:301–307PubMedCrossRefGoogle Scholar
  19. 19.
    Suzuki T, Okamura Y, Arakaki A et al (2007) Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1. FEBS Lett 581:3443–3448PubMedCrossRefGoogle Scholar
  20. 20.
    Suzuki T, Okamura Y, Calugay RJ et al (2006) Global gene expression analysis of iron-inducible genes in Magnetospirillum magneticum AMB-1. J Bacteriol 188:2275–2279PubMedCrossRefGoogle Scholar
  21. 21.
    Taoka A, Asada R, Sasaki H et al (2006) Spatial localizations of Mam22 and Mam12 in the magnetosomes of Magnetospirillum magnetotacticum. J Bacteriol 188:3805–3812PubMedCrossRefGoogle Scholar
  22. 22.
    Touati D (2000) Iron and oxidative stress in bacteria. Arch Biochem Biophys 373:1–6PubMedCrossRefGoogle Scholar
  23. 23.
    Yang C-D, Takeyama H, Tanaka T et al (2001) Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1. Enzyme Microb Technol 29:13–19PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Azuma Taoka
    • 1
  • Chihiro Umeyama
    • 1
  • Yoshihiro Fukumori
    • 1
  1. 1.Department of Life Science, Graduate School of Natural Science and TechnologyKanazawa UniversityKanazawaJapan

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