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Current Microbiology

, 59:227 | Cite as

An Endophytic Taxol-Producing Fungus from Taxus media, Cladosporium cladosporioides MD2

  • Peng Zhang
  • Peng-Peng Zhou
  • Long-Jiang YuEmail author
Article

Abstract

Fermentation processes using taxol-producing fungi other than Taxus spp. may be an alternative way to produce taxol, which is an important antitumor agent used widely in the clinic setting. In this study, a taxol-producing endophytic fungus strain MD2 was isolated from the inner bark of Taxus media. Strain MD2 produced taxol when grown in potato dextrose liquid medium. The fungal taxol—which was analyzed by ultraviolet, high-performance liquid chromatography and mass spectrometry—was shown to be identical to authentic taxol and 10-deacetylbaccatin III. Further analysis with nuclear magnetic resonance (NMR) spectroscopy to show the chemical structure of the fungal taxol indicated that the fungal taxol produced an NMR spectrum identical to that of authentic taxol. Strain MD2 was identified as Cladosporium cladosporioides according to morphology of the fungal culture, characteristics of the spores, and analysis of 18S rDNA sequence. In addition, 10-deacetylbaccatin III-10-O-acetyl transferase gene of C. cladosporioides MD2 was cloned for the first time and was shown to share 99% identity with that of T. x media and 97% identity with that of T. wallichiana var. mairei.

Keywords

Taxol Endophytic Fungus Cladosporium Cladosporium Cladosporioides Taxol Biosynthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant 20776058) and the New Century Talents Support Program by the Ministry of Education of China in 2006. We thank Gu Xiao-man and Cheng Hong for mass spectral analysis, Liang Ping for NMR analysis, and Gao Xian-Hui for acquiring the electron micrograph of C. cladosporioides MD2 spores. All three are members of the Analytic and Testing Center at Huazhong University of Science and Technology.

References

  1. 1.
    Daunter B, Greenshields RN (1973) Toxicity of Cladosporium cladosporioides. J Gen Microbiol 75:15Google Scholar
  2. 2.
    Edward H, Kerns KJ, Volk SE, Hill E (1994) Profiling taxanes in Taxus extracts using LC/MS and LC/MS/MS techniques. J Nat Prod 57:1391–1403CrossRefGoogle Scholar
  3. 3.
    Jennewein S, Wildung MR, Chau M, Walker K, Croteau R (2004) Random sequencing of an induced Taxus cell cDNA library for identification of clones involved in Taxol biosynthesis. Proc Natl Acad Sci USA 101:9149–9154PubMedCrossRefGoogle Scholar
  4. 4.
    Ji Y, Bi JN, Yan B, Zhu XD (2006) Taxol-producing fungi: A new approach to industrial production of taxol. Chin J Biotechnol 22:1–6CrossRefGoogle Scholar
  5. 5.
    Kim WK, Mauthe W (1990) Isolation of high molecular weight DNA and double-stranded RNAs from fungi [J]. Can J Bot 68:1898–1902Google Scholar
  6. 6.
    Li J, Strobel G, Sidhu R, Hess WM, Ford E (1996) Endophytic taxol-producing fungi from bald cypress, Taxodium distichum. Microbiology 142:2223–2226PubMedCrossRefGoogle Scholar
  7. 7.
    Stierle A, Stroble G, Stierle D (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science 260:214–216PubMedCrossRefGoogle Scholar
  8. 8.
    Strobel GA, Yang X, Sears J, Kramer R, Sidhu R, Hess WM (1996) Taxol from Pestalotiopsis microspora, an endophytic fungus from Taxus wallacbiana. Microbiology 142:435–440PubMedCrossRefGoogle Scholar
  9. 9.
    Tasic S, Tasic NM (2007) Cladosporium spp.—Cause of opportunistic mycoses. Acta Fac Med Naiss 24:15–19Google Scholar
  10. 10.
    Wakler K, Croteau R (2000) Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli. Proc Natl Acad Sci USA 97:583–587CrossRefGoogle Scholar
  11. 11.
    Wang J, Li G, Lu H, Zheng Z, Huang Y, Su W (2000) Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei. FEMS Microbiol Lett 193:249–253PubMedCrossRefGoogle Scholar
  12. 12.
    Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT (1971) Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc 93:2325–2327PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanChina
  2. 2.Key Laboratory of Molecular Biophysics, Ministry of Education, College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanChina

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