Current Microbiology

, Volume 58, Issue 1, pp 35–38 | Cite as

Biodegradation of Chlorpyrifos in Soil by Enriched Cultures



Three aerobic bacterial consortia, AC, BC, and DC, developed from pesticide-contaminated soils of Punjab were able to degrade chlorpyrifos after 21 days of incubation in basal medium by 54, 46, and 61% and chlorpyrifos (50 mg/L) in soil after 30 days by 50, 56, and 64%. Pseudomonas aeruginosa, Bacillus cereus, Klebsiella sp., and Serratia marscecens obtained from these consortia showed 84, 84, 81, and 80% degradation of chlorpyrifos (50 mg/L) in liquid medium after 20 days and 92, 60, 56, and 37% degradation of chlorpyrifos (50 mg/L) in soil after 30 days. Populations of Bacillus cereus, Klebsiella sp., and Serratia marscecens remained steady in soil experiments except for P. aeruginosa, where the population showed a substantial increase. Formation of 3,5,6-trichloro-2-pyridinol, the major metabolite of chlorpyrifos degradation, was observed during the degradation of chlorpyrifos by P. aeruginosa, which disappeared to negligible amounts.



This research was supported by a grant from the Department of Biotechnology (DBT), New Delhi, India.


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© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of Biotechnology and Environmental SciencesThapar Institute of Engineering and TechnologyPatialaIndia
  2. 2.Department of Biotechnology and BioinformaticsNIIT Institute of Information Technology (TNI)New DelhiIndia

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