Current Microbiology

, Volume 57, Issue 6, pp 631–637 | Cite as

The Lysis System of the Streptomyces aureofaciens Phage μ1/6

Article

Abstract

Previously, two genes, designated as lyt and hol, were identified in the lysis module of phage μ1/6. They were cloned and expressed in Escherichia coli. An additional candidate holin gene, hol2, was found downstream from the hol gene based on one predicted transmembrane domain and a highly charged C-terminal sequence of the encoded protein. Expression of hol or hol2 in E. coli was shown to cause cell death. The concomitant expression of λ endolysin (R) and μ1/6 holin resulted in cell lysis. Similarly, the coexpression of the endolysin and holin of phage μ1/6 led to lysis, apparently due to the ability of μ1/6 endolysin to hydrolyze the peptidoglycan layer of this bacterium. In contrast, the simultaneous expression of μ1/6 hol2 and the endolysin gene (λR or μ1/6 lyt) did not cause detectable lysis of the host cells. Demonstration of the holin function in streptomycetes was achieved by providing for the release of μ1/6 endolysin to the periplasm and subsequent cleavage of the peptidoglycan, which strongly suggested that the holin produces lesions in the streptomycete membrane.

Keywords

Apramycin Endolysin Thiostrepton Lysis Gene Concomitant Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This work was supported by VEGA grant no. 2/0121/25 of the Slovak Academy of Sciences.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Institute of Molecular BiologySlovak Academy of Sciences Bratislava Slovakia

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