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Current Microbiology

, Volume 57, Issue 5, pp 412–417 | Cite as

Physiological Alteration of the Marine Bacterium Vibrio angustum S14 Exposed to Simulated Sunlight During Growth

  • Maher Abboudi
  • Sabine Matallana Surget
  • Jean-François Rontani
  • Richard Sempéré
  • Fabien JouxEmail author
Article

Abstract

Growth experiments on the marine bacterium Vibrio angustum S14 were conducted under four light conditions using a solar simulator: visible light (V), V + ultraviolet A (UV-A), V + UV-A + UV-B radiation, and dark. Growth was inhibited mainly by UV-B and slightly by UV-A. UV-B radiation induced filaments containing multiple genome copies with low cyclobutane pyrimidine dimers. These cells did not show modifications in cellular fatty acid composition in comparison with dark control cultures and decreased in size by division after subsequent incubation in the dark. A large portion of the bacterial population grown under visible light showed an alteration in cellular DNA fluorescence as measured by flow cytometry after SYBR-Green I staining. This alteration was not aggravated by UV-A and was certainly due to a change in DNA topology rather than DNA deterioration because all the cells remained viable and their growth was not impaired. Ecological consequences of these observations are discussed.

Keywords

Vibrio Marine Bacterium Cellular Fatty Acid Cyclobutane Solar Incident Radiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank T. Douki for determination CPDs by HPLC-MS/MS for standard samples. We are grateful to N. West for improvement of the language. This study was funded by the CNRS-INSU through the UVECO and ATIPE projects and by a fellowship from the Syrian Atomic Energy Commission to M.A.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Maher Abboudi
    • 1
  • Sabine Matallana Surget
    • 2
  • Jean-François Rontani
    • 1
  • Richard Sempéré
    • 1
  • Fabien Joux
    • 2
    Email author
  1. 1.Laboratoire de Microbiologie Géochimie et Ecologie MarinesCNRS/INSU, UMR 6117, Centre d’Océanologie de Marseille, Université de la MéditerranéeMarseille Cedex 9France
  2. 2.UPMC Univ Paris 06, CNRS, UMR 7621, Laboratoire d’Océanographie Biologique de BanyulsBanyuls/merFrance

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