Physiological Alteration of the Marine Bacterium Vibrio angustum S14 Exposed to Simulated Sunlight During Growth
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Growth experiments on the marine bacterium Vibrio angustum S14 were conducted under four light conditions using a solar simulator: visible light (V), V + ultraviolet A (UV-A), V + UV-A + UV-B radiation, and dark. Growth was inhibited mainly by UV-B and slightly by UV-A. UV-B radiation induced filaments containing multiple genome copies with low cyclobutane pyrimidine dimers. These cells did not show modifications in cellular fatty acid composition in comparison with dark control cultures and decreased in size by division after subsequent incubation in the dark. A large portion of the bacterial population grown under visible light showed an alteration in cellular DNA fluorescence as measured by flow cytometry after SYBR-Green I staining. This alteration was not aggravated by UV-A and was certainly due to a change in DNA topology rather than DNA deterioration because all the cells remained viable and their growth was not impaired. Ecological consequences of these observations are discussed.
KeywordsVibrio Marine Bacterium Cellular Fatty Acid Cyclobutane Solar Incident Radiation
We thank T. Douki for determination CPDs by HPLC-MS/MS for standard samples. We are grateful to N. West for improvement of the language. This study was funded by the CNRS-INSU through the UVECO and ATIPE projects and by a fellowship from the Syrian Atomic Energy Commission to M.A.
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