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Current Microbiology

, Volume 57, Issue 4, pp 312–317 | Cite as

Isolation and Characterization of ACC Deaminase Gene from Two Plant Growth-Promoting Rhizobacteria

  • Venkadasamy Govindasamy
  • Murugesan Senthilkumar
  • Kishore Gaikwad
  • Kannepalli AnnapurnaEmail author
Article

Abstract

Lowering of plant ethylene by deamination of its immediate precursor 1-aminocyclopropane-1-carboxylate (ACC) is a key trait found in many rhizobacteria. We isolated and screened bacteria from the rhizosphere of wheat for their ACC-degrading ability. The ACC deaminase gene (acdS) isolated from two bacterial isolates through PCR amplification was cloned and sequenced. Nucleotide sequence alignment of these genes with previously reported genes of Pseudomonas sp. strain ACP and Enterobacter cloacae strain UW4 showed variation in their sequences. In the phylogenetic analysis, distinctness of these two genes was observed as a separate cluster. 16S rDNA sequencing of two isolates identified them to be Achromobacter sp. and Pseudomonas stutzeri.

Keywords

Polymerase Chain Reaction Amplification Pseudomonas Fluorescens Pseudomonas Stutzeri Nonspecific Band acdS Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors are thankful to Department of Science and Technology (DST), India, for the financial grant. The first author thanks Indian Agricultural Research Institute, New Delhi, India, for his PhD fellowship.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Venkadasamy Govindasamy
    • 1
  • Murugesan Senthilkumar
    • 1
  • Kishore Gaikwad
    • 2
  • Kannepalli Annapurna
    • 1
    Email author
  1. 1.Division of MicrobiologyIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.National Research Centre on Plant BiotechnologyIARINew DelhiIndia

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