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Current Microbiology

, Volume 57, Issue 3, pp 175–180 | Cite as

Use of a Cry1Ac-Resistant Line of Helicoverpa armigera (Lepidoptera: Noctuidae) to Detect Novel Insecticidal Toxin Genes in Bacillus thuringiensis

  • Cheryl E. Beard
  • Leon Court
  • Roslyn G. Mourant
  • Bill James
  • Jeroen Van Rie
  • Luke Masson
  • Raymond J. Akhurst
Article

Abstract

This paper describes a screening strategy incorporating resistant insect lines for discovery of new Bacillus thuringiensis toxins against a background of known genes that would normally mask the activity of additional genes and the application of that strategy. A line of Helicoverpa armigera with resistance to Cry1Ac (line ISOC) was used to screen Cry1Ac-expressing strains of B. thuringiensis for additional toxins with activity against H. armigera. Using this approach, a number of Cry1Ac-producing strains with significant toxicity toward Cry1Ac-resistant H. armigera were identified. When the insecticidal protein complement of one of these strains, C81, was examined in detail, a novel cry2 gene (cry2Af1) was detected.

Keywords

Bacillus Thuringiensis Cry1Ab Protein cry2Ab Gene cry1Ac Gene Cry1Ac Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Cheryl E. Beard
    • 1
  • Leon Court
    • 1
  • Roslyn G. Mourant
    • 1
  • Bill James
    • 1
  • Jeroen Van Rie
    • 2
  • Luke Masson
    • 3
  • Raymond J. Akhurst
    • 1
  1. 1.CSIRO EntomologyCanberraAustralia
  2. 2.Bayer BioScience N.VGentBelgium
  3. 3.National Research Council of Canada, Biotechnology Research InstituteQuébecCanada

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