Current Microbiology

, Volume 56, Issue 1, pp 55–60 | Cite as

Cadmium–Induced Siderophore Production by a High Cd-Resistant Bacterial Strain Relieved Cd Toxicity in Plants Through Root Colonization



This study focuses on the isolation and characterization of a high cadmium (Cd)-resistant bacterial strain, and possible exploitation of its Cd-accumulation and Cd-induced siderophore production property to improve plant growth in cadmium-contaminated soil through root colonization. The bacterial strain could tolerate up to 8 mM of Cd and could accumulate Cd intracellularly. The strain showed Cd-induced siderophore production maximally at 1.75 mM of Cd concentration under culture condition. It stimulated the growth of mustard and pumpkin plants in Cd-added soil through its establishment in rhizosphere. Through biochemical characterization and 16S rDNA sequence analysis, the strain KUCd1, as the name given to it, was identified as a strain of Pseudomonasaeruginosa.


  1. 1.
    Adriano DC (1986) Trace elements in the terrestrial environment. New York: Springer-Verlag, pp 106–155Google Scholar
  2. 2.
    Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410PubMedGoogle Scholar
  3. 3.
    Arnon DI (1949) Copper enzymes in isolated chloroplast: polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15PubMedGoogle Scholar
  4. 4.
    Bar-Ness E, Chen Y, Hadar H, Marschner H, Romheld V (1991) Siderophores of Pseudomonas putida as a iron source for dicot and monocot plants. Plant Soil 130:231–241CrossRefGoogle Scholar
  5. 5.
    Burd GI, Dixon GD, Glick BR (2000) Plant growth promoting bacteria that decrease heavy metal toxicity in plants. Can J Microbiol 46:237–245PubMedCrossRefGoogle Scholar
  6. 6.
    Camargo FAO, Bento FM, Okeke BC, Frankenberger WT (2003) Chromate reduction by chromium–resistant bacteria isolated from soils contaminated with dichromate. J Environ Qual 32:1228–1233PubMedCrossRefGoogle Scholar
  7. 7.
    Chen YH, Shen ZG, Li XD (2004) The use of vetiver grass (Vetiveria zizanioides) in the phytoremediation of soils contaminated with heavy metals. Appl Geochem 19:1553–1565CrossRefGoogle Scholar
  8. 8.
    Crowley DE, Reid CPP, Szaniszlo PJ (1988) Utilization of microbial siderophores in iron acquisition by oat. Plant Physiol 87:680–685PubMedCrossRefGoogle Scholar
  9. 9.
    Dao KHT, Hamer KE, Clark CL, Harshman LG (2001) Pyoverdine production by Pseudomonas aeruginosa exposed to metals or an oxidative stress. Ecol Appl 9:441–448CrossRefGoogle Scholar
  10. 10.
    Giovannoni SJ (1991) The polymerase chain reaction. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Chichester: Wiley, pp 177–203Google Scholar
  11. 11.
    Höfte M, Dong Q, Kourambas S, Krishnapillai V, Sherratt D, Mergeay M (1994) The sss gene product, which affects pyoverdin production in Pseudomonas aeruginosa 7NSK2, is a site-specific recombinase. Mol Microbiol 14:1011–1020PubMedCrossRefGoogle Scholar
  12. 12.
    Katiyar V, Goel R (2004) Siderophore mediated plant growth promotion at low temperature by mutant of fluorescent pseudomonad. Plant Growth Regul 42:239–244CrossRefGoogle Scholar
  13. 13.
    Meyer JM, Abdallah MA (1978) The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physiochemical properties. J Gen Microbiol 107:319–328Google Scholar
  14. 14.
    Mishra D, Kar M (1974) Nickel in plant growth and metabolism. Bot Rev 40:395–452CrossRefGoogle Scholar
  15. 15.
    Padmaja K, Prasad DDK, Prasad ARK (1990) Inhibition of chlorophyll synthesis in Phaseolus vulgaris seedlings by cadmium acetate. Photosynthetica 24:399–405Google Scholar
  16. 16.
    Palleroni NJ (1984) Family I. Pseudomonadaceae. In: Krieg NR, Holt JG (eds) Bergey’s manual of systematic bacteriology. Baltimore: Williams and Wilkins, pp 143–213Google Scholar
  17. 17.
    Pinto E, Sigaud-Kutner TCS, Leitão MAS, Okamoto OK, Morse D, Colepicolo P (2003) Heavy metal-induced oxidative stress in algae. J Phycol 39:1008–1018CrossRefGoogle Scholar
  18. 18.
    Rangarajan A, Kelly JF (1998) Iron bioavailability from Amaranthus species: 1. in vitro dialysable iron for estimation of genetic variation. J Sci Food Agric 78:267–273CrossRefGoogle Scholar
  19. 19.
    Sanitá di Toppi L, Gabbrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41:105–130CrossRefGoogle Scholar
  20. 20.
    Schwyn B, Neiland JB (1987) Universal chemical assay for the detection of siderophores. Anal Biochem 160:47–56PubMedCrossRefGoogle Scholar
  21. 21.
    Sheng XF, Xia JJ (2006) Improvement of rape (Brassica napus) plant growth and cadmium uptake by cadmium-resistant bacteria. Chemosphere 64:1036–1042PubMedCrossRefGoogle Scholar
  22. 22.
    Silver S, Phung LT (1996) Bacterial heavy metal resistance: new surprises. Annu Rev Microbiol 50:753–789PubMedCrossRefGoogle Scholar
  23. 23.
    Simons M, van der Bij AJ, Brand I, de Weger LA, Wijffelman CA, Lugtenberg BJJ (1996) Gnotobiotic system for studying rhizosphere colonization by plant growth- promoting Pseudomonas bacteria. Mol Plant-Microbe Interact 9:600–607PubMedGoogle Scholar
  24. 24.
    Singh PK, Tewari RK (2003) Cadmium toxicity induced changes in plant water relations and oxidative metabolism of Brassica juncea L plants. J Environ Biol 24:107–112PubMedGoogle Scholar
  25. 25.
    Tripathi M, Munot HP, Shouche Y, Meyer JM, Goel R (2005) Isolation and functional characterization of siderophore–producing lead- and cadmium-resistant Pseudomonas putida KNP9. Curr Microbiol 50:233–237PubMedCrossRefGoogle Scholar
  26. 26.
    Wang CL, Michels PC, Dawson SC, Kitisakkul S, Baross JA, Keasling JD, Clark DS (1997) Cadmium removal by a new strain of Pseudomonas aeruginosa in aerobic culture. Appl Environ Microbiol 63:4075–4078PubMedGoogle Scholar
  27. 27.
    Wang Y, Brown HW, Crowley DE, Szaniszlo PJ (1993) Evidence for direct utilization of a siderophore ferroxamine B, in axenially grown cucumber. Plant Cell Environ 16:579–585CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Department of MicrobiologyUniversity of KalyaniWest BengalIndia

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