Multilocus Sequence Typing of Uropathogenic ESBL-Producing Escherichia coli Isolated in a Brazilian Community
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In the present study, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) and polymerase chain reaction detection of three resistance genes were combined to characterize seven uropathogenic E. coli isolated from outpatients. Selected portions of seven housekeeping and three antibiotic-resistance genes of the isolates were sequenced. The seven isolates were classified into four different sequence types (STs) by MLST and five PGFE types. Three isolates had a novel allelic profile representing a new ST designated as ST528 and showed the same PFGE and resistance genes. Two isolates, both characterized as ST359, were differentiated by PFGE and shared only one of the antibiotic-resistance genes studied. Comparison of MLST results with those of PFGE and resistance genes demonstrated that Escherichia coli had acquired different antibiotic-resistance genes and DNA rearrangements, causing alterations in PFGE patterns but maintaining the same ST. Furthermore, this article also reports the first detection of a CTX-M-2 ESBL E. coli and SHV-5 in a Brazilian community.
KeywordsMultilocus Sequence Typing Fumarate Hydratase MLST Database MLST Scheme eBURST Analysis
We are grateful to Lemos Laboratórios de Análises Clínicas for supplying all isolates, to Hemocentro for sequencing, to Dr. David Livermore from the Central Public Health Laboratory, London, UK, and Dr. Jorge Sampaio from the Fleury-Centro de Medicina Diagnóstica, Brazil for gently providing the ESBL-producing E. coli controls. This study was financially supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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