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Cellular Responses and Proteomic Analysis of Escherichia coli Exposed to Green Tea Polyphenols

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Abstract

The purpose of this study was to characterize the cellular response and proteomic analysis of Escherichia coli exposed to tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis L). TPP showed a dose-dependent bactericidal effect on E. coli. Analysis of cell-membrane fatty acids of E. coli cultures treated with TPP identified unique changes in saturated and unsaturated fatty acids, whereas scanning electron microscopic analysis demonstrated the presence of perforations and irregular rod forms with wrinkled surfaces in cells treated with TPP. Two-dimensional polyacrylamide gel electrophoresis of soluble protein fractions from E. coli cultures exposed to TPP showed 17 protein spots increased or decreased by TPP. Nine upregulated proteins were identified (including GroEL and proteins involved in cellular defense, such as GyrA, RpoS, SodC, and EmrK), whereas the expression of eight proteins was downregulated by exposure to TPP (including proteins involved in carbon and energy metabolism, such as Eno, SdhA, and UgpQ, as well as those involved in amino-acid biosynthesis, such as GltK and TyrB). These results provide clues for understanding the mechanism of TPP-induced stress and cytotoxicity on E. coli.

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Acknowledgment

This research was accomplished while one of the authors (K. H. O.) was on sabbatical leave (2006 through 2007) from Soonchunhyang University.

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Authors and Affiliations

  1. Department of Biotechnology, Soonchunhyang University, P. O. Box 97, Asan, Chung-Nam, 336-600, Republic of Korea

    Y. S. Cho & K. H. Oh

  2. Division of Biomedical Sciences, University of California, Riverside, CA, 92521, USA

    N. L. Schiller & K. H. Oh

  3. Department of Environmental Education, Sunchon National University, Sunchon, Jeon-Nam, 540-742, Republic of Korea

    H. Y. Kahng

Authors
  1. Y. S. Cho
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  2. N. L. Schiller
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  3. H. Y. Kahng
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  4. K. H. Oh
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Correspondence to K. H. Oh.

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Cho, Y.S., Schiller, N.L., Kahng, H.Y. et al. Cellular Responses and Proteomic Analysis of Escherichia coli Exposed to Green Tea Polyphenols. Curr Microbiol 55, 501–506 (2007). https://doi.org/10.1007/s00284-007-9021-8

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  • DOI: https://doi.org/10.1007/s00284-007-9021-8

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