Current Microbiology

, Volume 55, Issue 4, pp 356–361 | Cite as

Porphyrobacter meromictius sp. nov., an Appendaged Bacterium, That Produces Bacteriochlorophyll a

  • Christopher Rathgeber
  • Natalia Yurkova
  • Erko Stackebrandt
  • Peter Schumann
  • Elaine Humphrey
  • J. Thomas Beatty
  • Vladimir Yurkov


Four Gram-negative strains (ML4T, ML19, ML31, ML32) of nonmotile, appendaged, budding bacteria were isolated from the meromictic Mahoney Lake in British Columbia, Canada. The strains were red to brown-red in color and produced bacteriochlorophyll a incorporated into photosynthetic pigment-protein complexes. Phylogenetic analysis has placed these strains within the class Alphaproteobacteria, with the closest relatives being members of the genera Erythrobacter, Porphyrobacter, and Erythromicrobium. Morphological features warrant their inclusion within the genus Porphyrobacter and these strains can be readily distinguished from other species of this genus on the basis of a mesophilic temperature range, a broad pH range, and tolerance to extremely high NaCl and Na2SO4 concentrations, in keeping with the environment from which they were isolated, a Na2SO4-dominated meromictic lake. These isolates utilize a variety of organic substrates for aerobic chemoheterotrophic growth and do not grow under anaerobic conditions, in either the presence or the absence of light. All strains require vitamin B12, and strains ML4T and ML19 require biotin. The DNA G + C contents ranged from 62.2 to 64.9 mol%. Phenotypic and phyletic data support the classification of strains ML4T, ML19, ML31, and ML32 as a novel Porphyrobacter species for which the name Porphyrobacter meromictius sp. nov. is proposed.



This research was funded by grants from the NSERC (Canada) to V.Y. and J.T.B. We thank K. J. Hall and T. G. Northcote for collection of samples from Mahoney Lake and H. G. Trüper for assistance with the nomenclature.

Literature Cited

  1. 1.
    Chung WK, King GM (2001) Isolation, characterization, and polyaromatic hydrocarbon degradation potential of aerobic bacteria from marine macrofaunal burrow sediments and description of Lutibacterium anuloederans gen. nov., sp. nov., and Cycloclasticus spirillensus sp. nov. Appl Env Microbiol 67:5585–5592CrossRefGoogle Scholar
  2. 2.
    Denner EBM, Vybiral D, Koblizek M, Kampfer P, Busse H-J, Velimirov B (2002) Erythrobacter citreus sp. nov., a yellow-pigmented bacterium that lacks bacteriochlorophyll a, isolated from the western Mediterranean Sea. Int J Syst Evol Microbiol 52:1655–1661PubMedCrossRefGoogle Scholar
  3. 3.
    De Soete G (1983) A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626CrossRefGoogle Scholar
  4. 4.
    Felsenstein J (1993) PHYLIP (phylogeny inference package), version 3.5.1. Department of Genetics, University of Washington, SeattleGoogle Scholar
  5. 5.
    Fuerst JA, Hawkins JA, Holmes A, Sly LI, Moore CJ, Stackebrandt E (1993) Porphyrobacter neustonensis gen. nov., sp. nov., an aerobic bacteriochlorophyll-synthesizing budding bacterium from fresh water. Int J Syst Bacteriol 43:125–134PubMedGoogle Scholar
  6. 6.
    Hall KJ, Northcote TG (1986) Conductivity-temperature standardization and dissolved solids estimation in a meromictic saline lake. Can J Fish Aquat Sci 43:2450–2454Google Scholar
  7. 7.
    Hanada S, Kawase Y, Hiraishi A, Takaichi S, Matsuura K, Shimada K, Nagashima KVP (1997) Porphyrobacter tepidarius sp. nov., a moderately thermophilic aerobic photosynthetic bacterium isolated from a hot spring. Int J Syst Bacteriol 47:408–413PubMedGoogle Scholar
  8. 8.
    Hiraishi A, Yonemitsu Y, Matsushita M, Shin YK, Kuraishi H, Kawahara K (2002) Characterization of Porphyrobacter sanguineus sp. nov., an aerobic bacteriochlorophyll-containing bacterium capable of degrading biphenyl and dibenzofuran. Arch Microbiol 178:45–52PubMedCrossRefGoogle Scholar
  9. 9.
    Imhoff JF (1988) Anoxygenic phototrophic bacteria. In: Austin B (ed) Methods in aquatic bacteriology. John Wiley and Sons, New York, pp 207–240Google Scholar
  10. 10.
    Maidak BL, Olsen GJ, Larsen N, Overbeek R, McCaughey MJ, Woese CR (1996) The Ribosomal Database Project (RDP). Nucleic Acids Res 24:82–85PubMedCrossRefGoogle Scholar
  11. 11.
    Mesbah M, Whitman WB (1989) Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr 479:297–306PubMedCrossRefGoogle Scholar
  12. 12.
    Northcote TG, Halsey TG (1969) Seasonal changes in the limnology of some meromictic lakes in southern British Columbia. J Fish Res Board Can 26:1763–1787Google Scholar
  13. 13.
    Rainey FA, Ward-Rainey N, Kroppenstedt RM, Stackebrandt E (1996) The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092PubMedCrossRefGoogle Scholar
  14. 14.
    Rainey FA, Silva J, Nobre MF, Silva MT, da Costa MS (2003) Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species. Int J Syst Evol Microbiol 53:35–41PubMedCrossRefGoogle Scholar
  15. 15.
    Rathgeber C, Beatty JT, Yurkov V (2004) Aerobic phototrophic bacteria: new evidence for the diversity, ecological importance and applied potential of this previously overlooked group. Photosynth Res 81:113–128CrossRefGoogle Scholar
  16. 16.
    Rathgeber C, Yurkova N, Stackebrandt E, Schumann P, Beatty JT, Yurkov V (2005) Roseicyclus mahoneyensis gen. nov., sp. nov., an aerobic phototrophic bacterium isolated from a meromictic lake. Int J Syst Evol Microbiol 55:1597–1603PubMedCrossRefGoogle Scholar
  17. 17.
    Shiba T, Simidu U (1982) Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. Int J Syst Bacteriol 32:211–217Google Scholar
  18. 18.
    Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128CrossRefGoogle Scholar
  19. 19.
    Yoon J-H, Kang KH, Oh T-K, Park Y-H (2004a) Erythrobacter aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54:1981–1985PubMedCrossRefGoogle Scholar
  20. 20.
    Yoon J-H, Lee M-H, Oh T-K (2004b) Porphyrobacter donghaensis sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 54:2231–2235PubMedCrossRefGoogle Scholar
  21. 21.
    Yurkov V, Beatty JT (1998) Aerobic anoxygenic phototrophic bacteria. Microbiol Mol Biol Rev 62:695–724PubMedGoogle Scholar
  22. 22.
    Yurkov VV, Csotonyi JT (2003) Aerobic anoxygenic phototrophs and heavy metalloid reducers from extreme environments. In: Pandalai SG (ed) Recent research developments in bacteriology. Transworld Research Network, Trivandrum, India, pp 247–300Google Scholar
  23. 23.
    Yurkov V, van Gemerden H (1993) Abundance and salt tolerance of obligately aerobic, phototrophic bacteria in a microbial mat. Neth J Sea Res 31:57–62CrossRefGoogle Scholar
  24. 24.
    Yurkov V, Stackebrandt E, Holmes A, Fuerst JA, Hugenholtz P, Golecki J, Gad’on N, Gorlenko VM, Kompantseva EI, Drews G (1994) Phylogenetic positions of novel aerobic, bacteriochlorophyll a containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44:427–434PubMedGoogle Scholar
  25. 25.
    Yurkova N, Rathgeber C, Swiderski J, Stackebrandt E, Beatty JT, Hall KJ, Yurkov V (2002) Diversity, distribution and physiology of the aerobic phototrophic bacteria in the mixolimnion of a meromictic lake. FEMS Microbiol Ecol 40:191–204CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Christopher Rathgeber
    • 1
  • Natalia Yurkova
    • 1
  • Erko Stackebrandt
    • 2
  • Peter Schumann
    • 2
  • Elaine Humphrey
    • 3
  • J. Thomas Beatty
    • 4
  • Vladimir Yurkov
    • 1
  1. 1.Department of MicrobiologyThe University of ManitobaWinnipegCanada
  2. 2.DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbHBraunschweigGermany
  3. 3.Bio-Imaging FacilityThe University of British ColumbiaVancouverCanada
  4. 4.Department of Microbiology and ImmunologyThe University of British ColumbiaVancouverCanada

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