Porphyrobacter meromictius sp. nov., an Appendaged Bacterium, That Produces Bacteriochlorophyll a
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Four Gram-negative strains (ML4T, ML19, ML31, ML32) of nonmotile, appendaged, budding bacteria were isolated from the meromictic Mahoney Lake in British Columbia, Canada. The strains were red to brown-red in color and produced bacteriochlorophyll a incorporated into photosynthetic pigment-protein complexes. Phylogenetic analysis has placed these strains within the class Alphaproteobacteria, with the closest relatives being members of the genera Erythrobacter, Porphyrobacter, and Erythromicrobium. Morphological features warrant their inclusion within the genus Porphyrobacter and these strains can be readily distinguished from other species of this genus on the basis of a mesophilic temperature range, a broad pH range, and tolerance to extremely high NaCl and Na2SO4 concentrations, in keeping with the environment from which they were isolated, a Na2SO4-dominated meromictic lake. These isolates utilize a variety of organic substrates for aerobic chemoheterotrophic growth and do not grow under anaerobic conditions, in either the presence or the absence of light. All strains require vitamin B12, and strains ML4T and ML19 require biotin. The DNA G + C contents ranged from 62.2 to 64.9 mol%. Phenotypic and phyletic data support the classification of strains ML4T, ML19, ML31, and ML32 as a novel Porphyrobacter species for which the name Porphyrobacter meromictius sp. nov. is proposed.
This research was funded by grants from the NSERC (Canada) to V.Y. and J.T.B. We thank K. J. Hall and T. G. Northcote for collection of samples from Mahoney Lake and H. G. Trüper for assistance with the nomenclature.
- 1.Chung WK, King GM (2001) Isolation, characterization, and polyaromatic hydrocarbon degradation potential of aerobic bacteria from marine macrofaunal burrow sediments and description of Lutibacterium anuloederans gen. nov., sp. nov., and Cycloclasticus spirillensus sp. nov. Appl Env Microbiol 67:5585–5592CrossRefGoogle Scholar
- 4.Felsenstein J (1993) PHYLIP (phylogeny inference package), version 3.5.1. Department of Genetics, University of Washington, SeattleGoogle Scholar
- 6.Hall KJ, Northcote TG (1986) Conductivity-temperature standardization and dissolved solids estimation in a meromictic saline lake. Can J Fish Aquat Sci 43:2450–2454Google Scholar
- 9.Imhoff JF (1988) Anoxygenic phototrophic bacteria. In: Austin B (ed) Methods in aquatic bacteriology. John Wiley and Sons, New York, pp 207–240Google Scholar
- 12.Northcote TG, Halsey TG (1969) Seasonal changes in the limnology of some meromictic lakes in southern British Columbia. J Fish Res Board Can 26:1763–1787Google Scholar
- 17.Shiba T, Simidu U (1982) Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a. Int J Syst Bacteriol 32:211–217Google Scholar
- 22.Yurkov VV, Csotonyi JT (2003) Aerobic anoxygenic phototrophs and heavy metalloid reducers from extreme environments. In: Pandalai SG (ed) Recent research developments in bacteriology. Transworld Research Network, Trivandrum, India, pp 247–300Google Scholar
- 24.Yurkov V, Stackebrandt E, Holmes A, Fuerst JA, Hugenholtz P, Golecki J, Gad’on N, Gorlenko VM, Kompantseva EI, Drews G (1994) Phylogenetic positions of novel aerobic, bacteriochlorophyll a containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44:427–434PubMedGoogle Scholar