Current Microbiology

, Volume 53, Issue 2, pp 102–107 | Cite as

Characterization of a Phosphate Solubilizing and Antagonistic Strain of Pseudomonas putida (B0) Isolated from a Sub-Alpine Location in the Indian Central Himalaya

  • Anita Pandey
  • Pankaj Trivedi
  • Bhavesh Kumar
  • Lok Man S. Palni


The morphological, biochemical, and physiological characteristics of a phosphate solubilizing and antagonistic bacterial strain, designated as B0, isolated from a sub-alpine Himalayan forest site have been described. The isolate is gram negative, rod shaped, 0.8 × 1.6 μm in size, and psychrotrophic in nature that could grow from 0 to 35°C (optimum temp. 25°C). It exhibited tolerance to a wide pH range (3–12; optimum 8.0) and salt concentration up to 4% (w/v). Although it was sensitive to kanamycin, gentamicin, and streptomycin (<10 μg mL−1), it showed resistance to higher concentrations of ampicillin, penicillin, and carbenicillin (>1000 μg mL−1). The isolate showed maximum similarity with Pseudomonas putida based on 16S rRNA analysis. It solubilized tricalcium phosphate under in vitro conditions. The phosphate solubilization was estimated along a temperature range (4–28°C), and maximum activity (247 μg mL−1) was recorded at 21°C after 15 days of incubation. The phosphate solubilizing activity coincided with a concomitant decrease in pH of the medium. The isolate also exhibited antifungal activity against phytopathogenic fungi in Petri dish assays and produced chitinase, ß-l,3-glucanase, salicylic acid, siderophore, and hydrogen cyanide. The plant growth promotion and antifungal properties were demonstrated through a maize-based bioassay under greenhouse conditions. Although the bacterial inoculation was found to result in significant increment in plant biomass, it stimulated bacterial and suppressed fungal counts in the rhizosphere. The present study is important with respect to enumerating microbial diversity of the colder regions as well as understanding the potential biotechnological applications of native microbes.


Chitinase Plant Growth Promotion Phosphate Solubilization Tricalcium Phosphate Alternaria Alternata 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The Department of Biotechnology, Council of Scientific and Industrial Research, and Union Ministry of Environment and Forests, Government of India, New Delhi, are thanked for financial support.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Anita Pandey
    • 1
  • Pankaj Trivedi
    • 1
  • Bhavesh Kumar
    • 1
  • Lok Man S. Palni
    • 1
    • 2
  1. 1.Environmental Physiology and BiotechnologyGB Pant Institute of Himalayan Environment and DevelopmentUttaranchalIndia
  2. 2.State Biotechnology ProgrammeGovt of Uttaranchal, Biotech BhavanUttaranchalIndia

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