Current Microbiology

, Volume 54, Issue 5, pp 361–365 | Cite as

Production and Regulation of Lipase Activity from Penicillium restrictum in Submerged and Solid-State Fermentations

  • Luciana A. I. de Azeredo
  • Patrícia M. Gomes
  • Geraldo L. Sant’AnnaJr.
  • Leda R. Castilho
  • Denise M. G. FreireEmail author


Different carbon (C) sources, mainly carbohydrates and lipids, have been screened for their capacity to support growth and lipase production by Penicillium restrictum in submerged fermentation (SmF) and in solid-state fermentation (SSF). Completely different physiological behaviors were observed after the addition of easily (oleic acid and glucose) and complex (olive oil and starch) assimilable C sources to the liquid and solid media. Maximal lipolytic activities (12.1 U/mL and 17.4 U/g) by P. restrictum were obtained with olive oil in SmF and in SSF, respectively. Biomass levels in SmF (12.2–14.1 mg/mL) and SSF (7.0–8.0 mg/g) did not varied greatly with the distinct C sources used. High lipase production (12.3 U/g) using glucose was only attained in SSF, perhaps due to the ability of this fermentation process to minimize catabolite repression.


Lipase Oleic Acid Lipase Activity Lipolytic Activity Lipase Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors acknowledge the financial support from FAPERJ, CNPq/PROFIX, CNPq/Universal.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Luciana A. I. de Azeredo
    • 1
  • Patrícia M. Gomes
    • 2
  • Geraldo L. Sant’AnnaJr.
    • 2
  • Leda R. Castilho
    • 2
  • Denise M. G. Freire
    • 1
    Email author
  1. 1.Biochemistry Department, Institute of ChemistryFederal University of Rio de Janeiro, Centro de TecnologiaRio de JaneiroBrazil
  2. 2.Chemical Engineering Program/COPPEFederal University of Rio de Janeiro, Centro de TecnologiaRio de JaneiroBrazil

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