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Current Microbiology

, Volume 55, Issue 2, pp 89–93 | Cite as

Expression Vectors for the Rapid Purification of Recombinant Proteins in Bacillus subtilis

  • Hoang Duc Nguyen
  • Trang Thi Phuong Phan
  • Wolfgang SchumannEmail author
Article

Abstract

We describe the construction of six novel plasmid-based IPTG-inducible expression vectors for Bacillus subtilis and related species. While one vector allows intracellular production of recombinant proteins, the second provides a strong secretion signal. The third vector allows addition of the c-Myc epitope tag, and the remaining three vectors provide the purification tags His and Strep. The versatility of all six vectors was demonstrated by the insertion of several reporter genes and by their regulated overexpression. Recombinant proteins with a His- or Strep-tag could be purified to near homogeneity in a single step.

Keywords

Recombinant Protein Listeria Monocytogenes Direct Repeat Structural Instability Small Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the DLR (VNB02/B03), the MOST (Life Science-643204), and the Bayerische Forschungsstiftung grants to T.T.P.P. All vectors can be ordered from Mobitec (http://www.mobitec.com).

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hoang Duc Nguyen
    • 1
  • Trang Thi Phuong Phan
    • 1
  • Wolfgang Schumann
    • 1
    Email author
  1. 1.Institute of GeneticsUniversity of BayreuthBayreuthGermany

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