Expression Vectors for the Rapid Purification of Recombinant Proteins in Bacillus subtilis
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We describe the construction of six novel plasmid-based IPTG-inducible expression vectors for Bacillus subtilis and related species. While one vector allows intracellular production of recombinant proteins, the second provides a strong secretion signal. The third vector allows addition of the c-Myc epitope tag, and the remaining three vectors provide the purification tags His and Strep. The versatility of all six vectors was demonstrated by the insertion of several reporter genes and by their regulated overexpression. Recombinant proteins with a His- or Strep-tag could be purified to near homogeneity in a single step.
KeywordsRecombinant Protein Listeria Monocytogenes Direct Repeat Structural Instability Small Plasmid
This work was supported by the DLR (VNB02/B03), the MOST (Life Science-643204), and the Bayerische Forschungsstiftung grants to T.T.P.P. All vectors can be ordered from Mobitec (http://www.mobitec.com).
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