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Current Microbiology

, Volume 54, Issue 2, pp 149–154 | Cite as

Mercury Resistance in Bacterial Strains Isolated from Tailing Ponds in a Gold Mining Area Near El Callao (Bolívar State, Venezuela)

  • María Mercedes Ball
  • Pablo Carrero
  • David Castro
  • Luis Andrés YarzábalEmail author
Article

Abstract

Bacterial resistance to mercury (Hg) was investigated in strains isolated from Hg-contaminated tailing ponds located in the gold mining area of El Callao (Bolívar State, Venezuela). High frequencies of resistance were detected to both inorganic-Hg and organomercurials among these strains. A broad range of resistance levels was observed when determining minimal inhibitory concentrations of Hg2+. Some strains were able to grow in liquid medium containing 25 μM Hg2+, whereas others grew at 300 μM Hg2+. Of 190 Hg-resistant strains tested, 58.2% were additionally shown to be resistant to ampicillin (40 mg/L), 33.3% to chloramphenicol (30 mg/L), 24.9% to streptomycin (30 mg/L), 23.3% to tetracycline (30 mg/L), and 1.6% to kanamycin (30 mg/L). Furthermore, we found that 20% of the Hg-resistant strains were simultaneously resistant to as many as four of these antibiotics, at the concentrations tested. The presence of large plasmids in 62.9% of 53 Hg-resistant strains screened prompted us to investigate the horizontal transfer of resistance determinants. Mating experiments were performed using Escherichia coli and Pseudomonas aeruginosa as recipient strains. The results obtained confirmed that indigenous Hg-resistant bacteria colonizing the tailing ponds can effectively transfer the phenotype to potentially pathogenic species.

Keywords

HgCl2 Venezuela Resistance Determinant Mating Experiment Recipient Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Ing. Elis Yegres and Lic. David Rondón (CVG-Minerven), as well as Ing. Máximo Benavides and María T. García (UNEXPO, Pto. Ordaz), for helping us during the collection of the samples. We are grateful to Luis Gutierrez (Lab. Espectroscopía Molecular, ULA) for the determination of soluble Hg by AAS and to Dr. Leonardo Serrano (Stanford University) for critical reading of the manuscript. This work was supported by Consejo de Desarrollo Científico, Humanístico y Tecnológico, CDCHT-ULA.

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • María Mercedes Ball
    • 1
  • Pablo Carrero
    • 2
  • David Castro
    • 1
  • Luis Andrés Yarzábal
    • 1
    Email author
  1. 1.Laboratorio de Microbiología Molecular y Biotecnología, Departamento de Biología, Facultad de CienciasNúcleo de La Hechicera, Universidad de Los AndesMéridaVenezuela
  2. 2.Laboratorio de Espectroscopía Molecular, Departamento de Química, Facultad de CienciasUniversidad de Los Andes, Núcleo de La HechiceraMéridaVenezuela

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