Current Microbiology

, Volume 53, Issue 5, pp 406–411

Acidianus manzaensis sp. nov., a Novel Thermoacidophilic Archaeon Growing Autotrophically by the Oxidation of H2 with the Reduction of Fe3+

  • Naoki Yoshida
  • Masanori Nakasato
  • Naoya Ohmura
  • Akikazu Ando
  • Hiroshi Saiki
  • Masaharu Ishii
  • Yasuo Igarashi
Article

Abstract

A novel thermoacidophilic iron-reducing Archaeon, strain NA−1, was isolated from a hot fumarole in Manza, Japan. Strain NA-1 could grow autotrophically using H2 or S0 as an electron donor and Fe3+ as an electron acceptor, and also could grow heterotrophically using some organic compounds. Fe3+ and O2 served as electron acceptors for growth. However, S0, NO3, NO2, SO42−, Mn4+, fumarate, and Fe2O3 did not serve as electron acceptors. The ranges of growth temperature and pH were 60–90°C (optimum: 80°C) and pH 1.0–5.0 (optimum: pH 1.2–1.5), respectively. Cells were nearly regular cocci with an envelope comprised of the cytoplasmic membrane and a single outer S-layer. The crenarchaeal-specific quinone (cardariellaquinone) was detected, and the genomic DNA G + C content was 29.9 mol%. From 16S rDNA analysis, it was determined that strain NA-1 is closely related to Acidianus ambivalens (93.1%) and Acidianus infernus (93.0%). However, differences revealed by phylogenetic and phenotypic analyses clearly show that strain NA-1 represents a new species, Acidianus manzaensis, sp. nov., making it the first identified thermoacidophilic iron-reducing microorganism (strain NA-1T = NBRC 100595 = ATCC BAA 1057).

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Naoki Yoshida
    • 1
    • 5
  • Masanori Nakasato
    • 2
  • Naoya Ohmura
    • 3
  • Akikazu Ando
    • 2
  • Hiroshi Saiki
    • 4
  • Masaharu Ishii
    • 1
  • Yasuo Igarashi
    • 1
  1. 1.Department of BiotechnologyGraduate School of Agricultural and Life Sciences, The University of TokyoTokyoJapan
  2. 2.Department of Bioresources ScienceGraduate School of Science and Technology, Chiba UniversityMatsudo CityJapan
  3. 3.Central Research Institute of Electric Power IndustryAbiko CityJapan
  4. 4.School of Bionics, Tokyo University of TechnologyTokyo
  5. 5.Central research institute of Electric Power IndustryAbiko cityJapan

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