A novel thermoacidophilic iron-reducing Archaeon, strain NA−1, was isolated from a hot fumarole in Manza, Japan. Strain NA-1 could grow autotrophically using H2 or S0 as an electron donor and Fe3+ as an electron acceptor, and also could grow heterotrophically using some organic compounds. Fe3+ and O2 served as electron acceptors for growth. However, S0, NO3−, NO2−, SO42−, Mn4+, fumarate, and Fe2O3 did not serve as electron acceptors. The ranges of growth temperature and pH were 60–90°C (optimum: 80°C) and pH 1.0–5.0 (optimum: pH 1.2–1.5), respectively. Cells were nearly regular cocci with an envelope comprised of the cytoplasmic membrane and a single outer S-layer. The crenarchaeal-specific quinone (cardariellaquinone) was detected, and the genomic DNA G + C content was 29.9 mol%. From 16S rDNA analysis, it was determined that strain NA-1 is closely related to Acidianus ambivalens (93.1%) and Acidianus infernus (93.0%). However, differences revealed by phylogenetic and phenotypic analyses clearly show that strain NA-1 represents a new species, Acidianus manzaensis, sp. nov., making it the first identified thermoacidophilic iron-reducing microorganism (strain NA-1T = NBRC 100595 = ATCC BAA 1057).
Iron Reduction Lactose Monohydrate Shewanella Putrefaciens Geobacter Sulfurreducens Anaerobic Bottle
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.
Brierley CL, Brierley JA (1973) A chemoautotrophic and thermophilic microorganism isolated from an acid hot spring. Can J Microbiol 19:183–188PubMedGoogle Scholar
Caccavo F Jr, Lonergan DJ, Lovley DR, Davis M, Stolz JF, McInerney MJ (1994) Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism. Appl Environ Microbiol 60(10):3752–3759PubMedGoogle Scholar
Dempsey GP, Bullivant S (1976) A copper block method for freezing non-cryoprotected tissue to produce ice-crystal-free regions for electron microscopy. J Microsc 106:251–260PubMedGoogle Scholar
Edward FD (1992) Archaea in coastal marine environments. Proc Natl Acad Sci USA 89:5685–5689CrossRefGoogle Scholar
Fuchs T, Huber H, Burggraf S, Stetter KO (1996) 16S rDNA-based phylogeny of the archeal order Sulfolobus and reclassification of Desulfurolobus ambivalens as Acidianus ambivalens comb. nov. Syst Appl Microbiol 19:56–60Google Scholar
He ZG, Zhong H, Li Y (2004) Acidianus tengchongensis sp. nov., a new species of acidothermophilic archaeon isolated from an acidothermal spring. Curr Microbiol 48(2):159–163PubMedCrossRefGoogle Scholar
Kashefi K, Holmes DE, Reysenbach AL, Lovley DR (2002) Use of Fe(III) as an electron acceptor to recover previously uncultured hyperthermophiles: isolation and characterization of Geothermobacterium ferrireducens gen. nov., sp. nov. Appl Environ Microbiol 68(4):1735–1742PubMedCrossRefGoogle Scholar
Kashefi K, Lovley DR (2000) Reduction of Fe(III), Mn(IV), and toxic metals at 100 degrees C by Pyrobaculum islandicum. Appl Environ Microbiol 66(3):1050–1056PubMedCrossRefGoogle Scholar
Kashefi K, Tor JM, Holmes DE, Gaw Van Praagh CV, Reysenbach AL, Lovley DR (2002) Geoglobus ahangari gen. nov., sp. nov., a novel hyperthermophilic archaeon capable of oxidizing organic acids and growing autotrophically on hydrogen with Fe(III) serving as the sole electron acceptor. Int J Syst Evol Microbiol 52(3):719–728PubMedCrossRefGoogle Scholar
Katayama-Fujimura Y, Komatsu Y, Kuraishi H, Kaneko T. (1984) Estimation of DNA base composition by high performance chromatography of its nuclease P1 hydrolysis. Agric Biol Chem 48:3169–3172Google Scholar
Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245PubMedCrossRefGoogle Scholar
Martin W, Russell MJ (2003) On the origins of cells: a hypothesis for the evolutionary transitions from abiotic geochemistry to chemoautotrophic prokaryotes, and from prokaryotes to nucleated cells. Phil Trans R Soc Lond B Biol Sci. 358:59–83CrossRefGoogle Scholar
Myers CR, Myers JM (1994) Ferric iron reduction-linked growth yields of Shewanella putrefaciens MR-1. J Appl Bacteriol 76(3):253–258PubMedGoogle Scholar
Ohmura N, Sasaki K, Matsumoto N, Saiki H (2002) Anaerobic respiration using Fe3+, S0, and H2 in the chemolithoautotrophic bacterium Acidithiobacillus ferrooxidans. J Bacteriol 184(8):2081–2087PubMedCrossRefGoogle Scholar
Segerer A, Neuner A, Kristjansson JK, Stetter KO (1986) Acidianus infernus gen. nov., sp. nov., and Acidianus brierleyi comb. nov.: facultatively aerobic, extremely acidophilic thermophilic sulfur-metabolizing archaebacteria. Int J Syst Bacteriol 36:559–564CrossRefGoogle Scholar
Thompson JC, Gibson TJ, Plewniak F, Leanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tool. Nucleic Acids Res 25:4876–4882PubMedCrossRefGoogle Scholar