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Current Microbiology

, Volume 53, Issue 5, pp 388–395 | Cite as

Toward a Wolbachia Multilocus Sequence Typing System: Discrimination of Wolbachia Strains Present in Drosophila Species

  • Charalampos Paraskevopoulos
  • Seth R. Bordenstein
  • Jennifer J. Wernegreen
  • John H. Werren
  • Kostas BourtzisEmail author
Article

Abstract

Among the diverse maternally inherited symbionts in arthropods, Wolbachia are the most common and infect over 20% of all species. In a departure from traditional genotyping or phylogenetic methods relying on single Wolbachia genes, the present study represents an initial Multilocus Sequence Typing (MLST) analysis to discriminate closely related Wolbachia pipientis strains, and additional data on sequence diversity in Wolbachia. We report a new phylogenetic characterization of four genes (aspC, atpD, sucB, and pdhB), and provide an expanded analysis of markers described in previous studies (16S rDNA, ftsZ, groEL, dnaA, and gltA). MLST analysis of the bacterial strains present in 16 different DrosophilaWolbachia associations detected four distinct clonal complexes that also corresponded to maximum-likelihood identified phylogenetic clades. Among the 16 associations analyzed, six could not be assigned to MLST clonal complexes and were also shown to be in conflict with relationships predicted by maximum-likelihood phylogenetic inferences. The results demonstrate the discriminatory power of MLST for identifying strains and clonal lineages of Wolbachia and provide a robust foundation for studying the ecology and evolution of this widespread endosymbiont.

Keywords

Clonal Complex Wolbachia Strain Allelic Profile MLST Scheme Wolbachia Surface Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors would like to thank Laura Baldo for her constructive comments and suggestions. The D. simulans (wMa) and D. innubila (wDin) were kindly provided by Prof. Bill Ballard and Prof. John Jaenike, respectively. This work was partially supported by intramural funds of the University of Ioannina to K. Bourtzis, by grants to J.J. Wernegreen from the National Institutes of Health (R01 GM62626-01) and the NASA Astrobiology Institute (NNA04CC04A), and to J.H. Werren and J.J. Wernegreen from the National Science Foundation (EF-0328363). This work was performed in part while S.R. Bordenstein held a National Research Council Research Associateship Award at the Marine Biological Laboratory.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Charalampos Paraskevopoulos
    • 1
  • Seth R. Bordenstein
    • 2
  • Jennifer J. Wernegreen
    • 2
  • John H. Werren
    • 3
  • Kostas Bourtzis
    • 1
    Email author
  1. 1.Department of Environmental and Natural Resources ManagementUniversity of IoanninaAgrinioGreece
  2. 2.Josephine Bay Paul Center for Comparative Molecular Biology and EvolutionThe Marine Biological LaboratoryWoods HoleUSA
  3. 3.Department of BiologyUniversity of RochesterRochesterUSA

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